Composite polyelectrolyte multilayer and mesoporous bioactive glass nanoparticle coating on 316L stainless steel for controlled antibiotic release and biocompatibility

Xinrui Liu, Hsiang Ho Chen, Yu Chien Lin, Sasza Chyntara Nabilla, Wai Ching Liu, Wen Chi Wang, Shao Ju Shih, Yunqian Li, Ching Po Lin, Gang Zhao, Ren Jei Chung

Research output: Contribution to journalArticle

Abstract

Bacterial infection in wounds or implants can cause osteomyelitis, eventually leading to orthopedic implant failure. In this study, polyelectrolyte multilayer (PEM) coating comprising collagen as the cationic layer, chitosan as the barrier layer and γ-poly-glutamic acid as the anionic layer were fabricated onto a 316L stainless steel substrate by spin coating technique. Tetracycline-loaded 57S mesoporous bioactive glass nanoparticles (57S MBG, SiO 2 :CaO:P 2 O 5 = 57:33:10 by wt%) were introduced into the γ-poly-glutamic acid layers. Herein, 57S MBG nanoparticles were successfully incorporated into the PEMs with a total thickness of ∼53 μm on 316L stainless steel (SS-PEMs-57S), which exhibited good hydrophilicity with a contact angle of 18.71°. The hardness of SS-PEMs-57S was 0.66 GPa while the Young's modulus was 11.5 GPa; these values are similar to those for the cortical bone. The surface roughness of MBG nanoparticle-incorporated PEMs increased from 231 to 384 nm. Controlled release of tetracycline loaded in MBG nanoparticles resulted in sustained antibacterial effect for up to 7 days, with higher release efficacy at low pH, which may be induced by inflammation or infection. Tetracycline loaded in SS-PEMs-57S showed good bacterial inhibition and maintained good cell viability in rat bone marrow mesenchymal stem cells (BMSCs) in the MTT assay. Moreover, SS-PEMs-57S also promoted mineralization of BMSCs. Therefore, this surface modification technology has great potential for endowing orthopedic implants with antibacterial and osteoconductive properties.

Original languageEnglish
Pages (from-to)725-735
Number of pages11
JournalJournal of Biomedical Nanotechnology
Volume14
Issue number4
DOIs
Publication statusPublished - Apr 1 2018

Fingerprint

Bioactive glass
Stainless Steel
Antibiotics
Polyelectrolytes
Biocompatibility
Nanoparticles
Glass
Multilayers
Stainless steel
Tetracycline
Anti-Bacterial Agents
Coatings
Bone
Composite materials
Orthopedics
Stem cells
Mesenchymal Stromal Cells
Glutamic Acid
Bone Marrow
Coating techniques

Keywords

  • 316L stainless steel
  • Bioactive glass
  • Bone marrow mesenchymal stem cells
  • Polyelectrolyte multilayers
  • Tetracycline

ASJC Scopus subject areas

  • Bioengineering
  • Medicine (miscellaneous)
  • Biomedical Engineering
  • Materials Science(all)
  • Pharmaceutical Science

Cite this

Composite polyelectrolyte multilayer and mesoporous bioactive glass nanoparticle coating on 316L stainless steel for controlled antibiotic release and biocompatibility. / Liu, Xinrui; Chen, Hsiang Ho; Lin, Yu Chien; Nabilla, Sasza Chyntara; Liu, Wai Ching; Wang, Wen Chi; Shih, Shao Ju; Li, Yunqian; Lin, Ching Po; Zhao, Gang; Chung, Ren Jei.

In: Journal of Biomedical Nanotechnology, Vol. 14, No. 4, 01.04.2018, p. 725-735.

Research output: Contribution to journalArticle

Liu, Xinrui ; Chen, Hsiang Ho ; Lin, Yu Chien ; Nabilla, Sasza Chyntara ; Liu, Wai Ching ; Wang, Wen Chi ; Shih, Shao Ju ; Li, Yunqian ; Lin, Ching Po ; Zhao, Gang ; Chung, Ren Jei. / Composite polyelectrolyte multilayer and mesoporous bioactive glass nanoparticle coating on 316L stainless steel for controlled antibiotic release and biocompatibility. In: Journal of Biomedical Nanotechnology. 2018 ; Vol. 14, No. 4. pp. 725-735.
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abstract = "Bacterial infection in wounds or implants can cause osteomyelitis, eventually leading to orthopedic implant failure. In this study, polyelectrolyte multilayer (PEM) coating comprising collagen as the cationic layer, chitosan as the barrier layer and γ-poly-glutamic acid as the anionic layer were fabricated onto a 316L stainless steel substrate by spin coating technique. Tetracycline-loaded 57S mesoporous bioactive glass nanoparticles (57S MBG, SiO 2 :CaO:P 2 O 5 = 57:33:10 by wt{\%}) were introduced into the γ-poly-glutamic acid layers. Herein, 57S MBG nanoparticles were successfully incorporated into the PEMs with a total thickness of ∼53 μm on 316L stainless steel (SS-PEMs-57S), which exhibited good hydrophilicity with a contact angle of 18.71°. The hardness of SS-PEMs-57S was 0.66 GPa while the Young's modulus was 11.5 GPa; these values are similar to those for the cortical bone. The surface roughness of MBG nanoparticle-incorporated PEMs increased from 231 to 384 nm. Controlled release of tetracycline loaded in MBG nanoparticles resulted in sustained antibacterial effect for up to 7 days, with higher release efficacy at low pH, which may be induced by inflammation or infection. Tetracycline loaded in SS-PEMs-57S showed good bacterial inhibition and maintained good cell viability in rat bone marrow mesenchymal stem cells (BMSCs) in the MTT assay. Moreover, SS-PEMs-57S also promoted mineralization of BMSCs. Therefore, this surface modification technology has great potential for endowing orthopedic implants with antibacterial and osteoconductive properties.",
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AU - Nabilla, Sasza Chyntara

AU - Liu, Wai Ching

AU - Wang, Wen Chi

AU - Shih, Shao Ju

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AU - Lin, Ching Po

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