Compartment-specific transcriptomics in a reef-building coral exposed to elevated temperatures

Anderson B. Mayfield, Yu Bin Wang, Chii Shiarng Chen, Chung Yen Lin, Shu Hwa Chen

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Although rising ocean temperatures threaten scleractinian corals and the reefs they construct, certain reef corals can acclimate to elevated temperatures to which they are rarely exposed in situ. Specimens of the model Indo-Pacific reef coral Pocillopora damicornis collected from upwelling reefs of Southern Taiwan were previously found to have survived a 36-week exposure to 30C, a temperature they encounter infrequently and one that can elicit the breakdown of the coral-dinoflagellate (genus Symbiodinium) endosymbiosis in many corals of the Pacific Ocean. To gain insight into the subcellular pathways utilized by both the coral hosts and their mutualistic Symbiodinium populations to acclimate to this temperature, mRNAs from both control (27C) and high (30C)-temperature samples were sequenced on an Illumina platform and assembled into a 236 435-contig transcriptome. These P. damicornis specimens were found to be ~60% anthozoan and 40% microbe (Symbiodinium, other eukaryotic microbes, and bacteria), from an mRNA-perspective. Furthermore, a significantly higher proportion of genes from the Symbiodinium compartment were differentially expressed after two weeks of exposure. Specifically, at elevated temperatures, Symbiodinium populations residing within the coral gastrodermal tissues were more likely to up-regulate the expression of genes encoding proteins involved in metabolism than their coral hosts. Collectively, these transcriptome-scale data suggest that the two members of this endosymbiosis have distinct strategies for acclimating to elevated temperatures that are expected to characterize many of Earth's coral reefs in the coming decades.

Original languageEnglish
Pages (from-to)5816-5830
Number of pages15
JournalMolecular Ecology
Volume23
Issue number23
DOIs
Publication statusPublished - Dec 1 2014
Externally publishedYes

Keywords

  • acclimation
  • coral reef
  • dinoflagellate
  • endosymbiosis
  • gene expression
  • Symbiodinium
  • transcriptome

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Genetics

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