Comparisons of in vitro antioxidant activities of storage proteins in tuber of two Dioscorea species

Yuh Hwa Liu, Hong Jen Liang, Huey Chuan Cheng, Yen Wenn Liu, Wen Chi Hou

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Dioscorin was purified by DE-52 ion exchange chromatography from two yam species, Dioscorea alata L. cv. Tainong 1 (TN1) and Dioscorea batatas Decne (imported from Japan, JP). By different in vitro antioxidant tests, including DPPH radical and hydroxyl radical scavenging activity assay, a reducing power test, an anti-lipid peroxidation test, DNA damage protection, and inhibition of dihydrorhodamine 123 oxidation by peroxynitrite, it was shown that dioscorins from the two species exhibited different scavenging activities against DPPH and hydroxyl radicals, even after heating 100°C for 5 min. Dioscorins from TN1 were hydrolyzed by pepsin for different durations and the peptic hydrolysates exhibited DPPH radical scavenging activities. Peptic hydrolysates separated by Sephadex G-50 (F) gel filtration were tested for anti-DPPH radical activity. Results showed that fractions of smaller molecular weight still have antioxidant activities.

Original languageEnglish
Pages (from-to)231-237
Number of pages7
JournalBotanical Studies
Volume47
Issue number3
Publication statusPublished - Jul 2006

Fingerprint

Dioscorea
storage proteins
tubers
antioxidant activity
hydroxyl radicals
hydrolysates
digestion
Dioscorea polystachya
Dioscorea alata
yams
testing
ion exchange chromatography
pepsin
DNA damage
lipid peroxidation
gels
oxidation
Japan
molecular weight
heat

Keywords

  • Antioxidant
  • Dioscorin
  • Peptic hydrolysates
  • Storage protein
  • Yam

ASJC Scopus subject areas

  • Plant Science

Cite this

Comparisons of in vitro antioxidant activities of storage proteins in tuber of two Dioscorea species. / Liu, Yuh Hwa; Liang, Hong Jen; Cheng, Huey Chuan; Liu, Yen Wenn; Hou, Wen Chi.

In: Botanical Studies, Vol. 47, No. 3, 07.2006, p. 231-237.

Research output: Contribution to journalArticle

Liu, Yuh Hwa ; Liang, Hong Jen ; Cheng, Huey Chuan ; Liu, Yen Wenn ; Hou, Wen Chi. / Comparisons of in vitro antioxidant activities of storage proteins in tuber of two Dioscorea species. In: Botanical Studies. 2006 ; Vol. 47, No. 3. pp. 231-237.
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