Comparison of virulence variations on MDCK monolayers by escherichia coli isolated from acute lobar nephroma and acute pyelonephritis

Chi Hui Cheng, Lin Hui Su, Yong Kwei Tsau, Tzou Yien Lin

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Escherichia coli is the most common pathogen associated with acute lobar nephroma (ALN), a clinically more severe parenchymal inflammatory disease that requires a longer duration of antibiotic treatment than acute pyelonephritis (APN). This study was conducted to unravel the virulence differences between clinical isolates of E. coli from pediatric ALN and APN patients. A total of 88 urinary isolates of E. coli were investigated. They were identified from ra-diologically diagnosed ALN and APN patients and had previously been molecularly characterized for important urovirulence genes. Madin-Darby canine kidney (MDCK) epithelial cells were used as an in vitro model. Multivariate logistic regression analyses indicated that ALN isolates were more likely to show adhesion (p<0.05; odds ratio [OR], 3.81; 95% confidence interval [CI], 1.23-11.80) and cytotoxicity (p<0.001; OR, 10.42; 95% CI, 3.03-35.89). However, no difference in the penetration ability was noted. Henceforth, the ability to adhere to and produce cytotoxicity against uroepithelial cells appears a prerequisite factor for E. coli to cause more severe bacterial kidney infection, such as ALN.

Original languageEnglish
Pages (from-to)65-72
Number of pages8
JournalNew Microbiologica
Volume34
Issue number1
Publication statusPublished - Jan 2011
Externally publishedYes

Fingerprint

Pyelonephritis
Virulence
Canidae
Escherichia coli
Kidney
Odds Ratio
Confidence Intervals
Madin Darby Canine Kidney Cells
Bacterial Infections
Logistic Models
Epithelial Cells
Regression Analysis
Pediatrics
Anti-Bacterial Agents
Genes
Therapeutics

Keywords

  • Acute focal bacterial nephritis
  • Bacterial adhesion
  • Cytotoxicity
  • MDCK
  • Virulence

ASJC Scopus subject areas

  • Microbiology (medical)

Cite this

Comparison of virulence variations on MDCK monolayers by escherichia coli isolated from acute lobar nephroma and acute pyelonephritis. / Cheng, Chi Hui; Su, Lin Hui; Tsau, Yong Kwei; Lin, Tzou Yien.

In: New Microbiologica, Vol. 34, No. 1, 01.2011, p. 65-72.

Research output: Contribution to journalArticle

@article{ae398adb94684a14bb85caf87881eca3,
title = "Comparison of virulence variations on MDCK monolayers by escherichia coli isolated from acute lobar nephroma and acute pyelonephritis",
abstract = "Escherichia coli is the most common pathogen associated with acute lobar nephroma (ALN), a clinically more severe parenchymal inflammatory disease that requires a longer duration of antibiotic treatment than acute pyelonephritis (APN). This study was conducted to unravel the virulence differences between clinical isolates of E. coli from pediatric ALN and APN patients. A total of 88 urinary isolates of E. coli were investigated. They were identified from ra-diologically diagnosed ALN and APN patients and had previously been molecularly characterized for important urovirulence genes. Madin-Darby canine kidney (MDCK) epithelial cells were used as an in vitro model. Multivariate logistic regression analyses indicated that ALN isolates were more likely to show adhesion (p<0.05; odds ratio [OR], 3.81; 95{\%} confidence interval [CI], 1.23-11.80) and cytotoxicity (p<0.001; OR, 10.42; 95{\%} CI, 3.03-35.89). However, no difference in the penetration ability was noted. Henceforth, the ability to adhere to and produce cytotoxicity against uroepithelial cells appears a prerequisite factor for E. coli to cause more severe bacterial kidney infection, such as ALN.",
keywords = "Acute focal bacterial nephritis, Bacterial adhesion, Cytotoxicity, MDCK, Virulence",
author = "Cheng, {Chi Hui} and Su, {Lin Hui} and Tsau, {Yong Kwei} and Lin, {Tzou Yien}",
year = "2011",
month = "1",
language = "English",
volume = "34",
pages = "65--72",
journal = "New Microbiologica",
issn = "1121-7138",
publisher = "Luigi Ponzio e figlio Editori",
number = "1",

}

TY - JOUR

T1 - Comparison of virulence variations on MDCK monolayers by escherichia coli isolated from acute lobar nephroma and acute pyelonephritis

AU - Cheng, Chi Hui

AU - Su, Lin Hui

AU - Tsau, Yong Kwei

AU - Lin, Tzou Yien

PY - 2011/1

Y1 - 2011/1

N2 - Escherichia coli is the most common pathogen associated with acute lobar nephroma (ALN), a clinically more severe parenchymal inflammatory disease that requires a longer duration of antibiotic treatment than acute pyelonephritis (APN). This study was conducted to unravel the virulence differences between clinical isolates of E. coli from pediatric ALN and APN patients. A total of 88 urinary isolates of E. coli were investigated. They were identified from ra-diologically diagnosed ALN and APN patients and had previously been molecularly characterized for important urovirulence genes. Madin-Darby canine kidney (MDCK) epithelial cells were used as an in vitro model. Multivariate logistic regression analyses indicated that ALN isolates were more likely to show adhesion (p<0.05; odds ratio [OR], 3.81; 95% confidence interval [CI], 1.23-11.80) and cytotoxicity (p<0.001; OR, 10.42; 95% CI, 3.03-35.89). However, no difference in the penetration ability was noted. Henceforth, the ability to adhere to and produce cytotoxicity against uroepithelial cells appears a prerequisite factor for E. coli to cause more severe bacterial kidney infection, such as ALN.

AB - Escherichia coli is the most common pathogen associated with acute lobar nephroma (ALN), a clinically more severe parenchymal inflammatory disease that requires a longer duration of antibiotic treatment than acute pyelonephritis (APN). This study was conducted to unravel the virulence differences between clinical isolates of E. coli from pediatric ALN and APN patients. A total of 88 urinary isolates of E. coli were investigated. They were identified from ra-diologically diagnosed ALN and APN patients and had previously been molecularly characterized for important urovirulence genes. Madin-Darby canine kidney (MDCK) epithelial cells were used as an in vitro model. Multivariate logistic regression analyses indicated that ALN isolates were more likely to show adhesion (p<0.05; odds ratio [OR], 3.81; 95% confidence interval [CI], 1.23-11.80) and cytotoxicity (p<0.001; OR, 10.42; 95% CI, 3.03-35.89). However, no difference in the penetration ability was noted. Henceforth, the ability to adhere to and produce cytotoxicity against uroepithelial cells appears a prerequisite factor for E. coli to cause more severe bacterial kidney infection, such as ALN.

KW - Acute focal bacterial nephritis

KW - Bacterial adhesion

KW - Cytotoxicity

KW - MDCK

KW - Virulence

UR - http://www.scopus.com/inward/record.url?scp=79952470937&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79952470937&partnerID=8YFLogxK

M3 - Article

VL - 34

SP - 65

EP - 72

JO - New Microbiologica

JF - New Microbiologica

SN - 1121-7138

IS - 1

ER -