Comparison of urovirulence factors and genotypes for bacteria causing acute lobar nephronia and acute pyelonephritis

Chi Hui Cheng, Yong Kwei Tsau, Lin Hui Su, Chia Ling Lin, Tzou Yien Lin

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

BACKGROUND: Acute lobar nephronia (ALN) is a severe renal parenchymal inflammatory disease that has been diagnosed with increasing frequency as a result of newer noninvasive diagnostic modalities. Escherichia coli is the most common bacterial pathogen isolated from the urine samples of ALN patients. This prospective study was conducted to elucidate and distinguish the bacterial virulence factors associated with ALN and acute pyelonephritis (APN) in pediatric patients. METHODS: Patients included in the present study were those suspected of an upper urinary tract infection who underwent ultrasonographic, computed tomographic and technetium 99m-dimercaptosuccinic acid scintigraphic evaluation to distinguish between ALN and APN. The E. coli isolates from the urine samples of patients were screened with polymerase chain reaction analysis for various urovirulence genes. Pulsed-field gel electrophoresis was used to analyze the genetic association of the isolates. RESULTS: A total of 88 patients were enrolled. Forty-six patients were diagnosed with ALN and 42 with APN. Demographic characteristics and clinical results were similar except for longer fever duration before admission, longer fever continuation following antibiotic treatment and higher C-reactive protein values noted in the ALN group. Diverse genotypes were found among the E. coli isolates in both groups. Among the pathogenetic determinants examined, multivariate logistic regression analysis indicated that a papG II allele was the only significant urovirulence factor associated with ALN (P < 0.005; odds ratio, 17.16). This association was independent of the presence of VUR. CONCLUSIONS: While no specific genetic lineage was identified among the E. coli isolates studied, a papG II gene was found to be strongly associated with the cause of ALN among pediatric patients.

Original languageEnglish
Pages (from-to)228-232
Number of pages5
JournalPediatric Infectious Disease Journal
Volume26
Issue number3
DOIs
Publication statusPublished - Mar 2007
Externally publishedYes

Fingerprint

Pyelonephritis
Genotype
Bacteria
Escherichia coli
Fever
Technetium Tc 99m Dimercaptosuccinic Acid
Urine
Pediatrics
Pulsed Field Gel Electrophoresis
Virulence Factors
Urinary Tract Infections
C-Reactive Protein
Genes
Logistic Models
Alleles
Odds Ratio
Regression Analysis
Demography
Prospective Studies
Anti-Bacterial Agents

Keywords

  • Acute focal bacterial nephritis
  • P-fimbriae adhesin
  • Polymerase chain reaction
  • Pulsed field gel electrophoresis
  • Urovirulence gene

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health
  • Microbiology (medical)

Cite this

Comparison of urovirulence factors and genotypes for bacteria causing acute lobar nephronia and acute pyelonephritis. / Cheng, Chi Hui; Tsau, Yong Kwei; Su, Lin Hui; Lin, Chia Ling; Lin, Tzou Yien.

In: Pediatric Infectious Disease Journal, Vol. 26, No. 3, 03.2007, p. 228-232.

Research output: Contribution to journalArticle

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abstract = "BACKGROUND: Acute lobar nephronia (ALN) is a severe renal parenchymal inflammatory disease that has been diagnosed with increasing frequency as a result of newer noninvasive diagnostic modalities. Escherichia coli is the most common bacterial pathogen isolated from the urine samples of ALN patients. This prospective study was conducted to elucidate and distinguish the bacterial virulence factors associated with ALN and acute pyelonephritis (APN) in pediatric patients. METHODS: Patients included in the present study were those suspected of an upper urinary tract infection who underwent ultrasonographic, computed tomographic and technetium 99m-dimercaptosuccinic acid scintigraphic evaluation to distinguish between ALN and APN. The E. coli isolates from the urine samples of patients were screened with polymerase chain reaction analysis for various urovirulence genes. Pulsed-field gel electrophoresis was used to analyze the genetic association of the isolates. RESULTS: A total of 88 patients were enrolled. Forty-six patients were diagnosed with ALN and 42 with APN. Demographic characteristics and clinical results were similar except for longer fever duration before admission, longer fever continuation following antibiotic treatment and higher C-reactive protein values noted in the ALN group. Diverse genotypes were found among the E. coli isolates in both groups. Among the pathogenetic determinants examined, multivariate logistic regression analysis indicated that a papG II allele was the only significant urovirulence factor associated with ALN (P < 0.005; odds ratio, 17.16). This association was independent of the presence of VUR. CONCLUSIONS: While no specific genetic lineage was identified among the E. coli isolates studied, a papG II gene was found to be strongly associated with the cause of ALN among pediatric patients.",
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N2 - BACKGROUND: Acute lobar nephronia (ALN) is a severe renal parenchymal inflammatory disease that has been diagnosed with increasing frequency as a result of newer noninvasive diagnostic modalities. Escherichia coli is the most common bacterial pathogen isolated from the urine samples of ALN patients. This prospective study was conducted to elucidate and distinguish the bacterial virulence factors associated with ALN and acute pyelonephritis (APN) in pediatric patients. METHODS: Patients included in the present study were those suspected of an upper urinary tract infection who underwent ultrasonographic, computed tomographic and technetium 99m-dimercaptosuccinic acid scintigraphic evaluation to distinguish between ALN and APN. The E. coli isolates from the urine samples of patients were screened with polymerase chain reaction analysis for various urovirulence genes. Pulsed-field gel electrophoresis was used to analyze the genetic association of the isolates. RESULTS: A total of 88 patients were enrolled. Forty-six patients were diagnosed with ALN and 42 with APN. Demographic characteristics and clinical results were similar except for longer fever duration before admission, longer fever continuation following antibiotic treatment and higher C-reactive protein values noted in the ALN group. Diverse genotypes were found among the E. coli isolates in both groups. Among the pathogenetic determinants examined, multivariate logistic regression analysis indicated that a papG II allele was the only significant urovirulence factor associated with ALN (P < 0.005; odds ratio, 17.16). This association was independent of the presence of VUR. CONCLUSIONS: While no specific genetic lineage was identified among the E. coli isolates studied, a papG II gene was found to be strongly associated with the cause of ALN among pediatric patients.

AB - BACKGROUND: Acute lobar nephronia (ALN) is a severe renal parenchymal inflammatory disease that has been diagnosed with increasing frequency as a result of newer noninvasive diagnostic modalities. Escherichia coli is the most common bacterial pathogen isolated from the urine samples of ALN patients. This prospective study was conducted to elucidate and distinguish the bacterial virulence factors associated with ALN and acute pyelonephritis (APN) in pediatric patients. METHODS: Patients included in the present study were those suspected of an upper urinary tract infection who underwent ultrasonographic, computed tomographic and technetium 99m-dimercaptosuccinic acid scintigraphic evaluation to distinguish between ALN and APN. The E. coli isolates from the urine samples of patients were screened with polymerase chain reaction analysis for various urovirulence genes. Pulsed-field gel electrophoresis was used to analyze the genetic association of the isolates. RESULTS: A total of 88 patients were enrolled. Forty-six patients were diagnosed with ALN and 42 with APN. Demographic characteristics and clinical results were similar except for longer fever duration before admission, longer fever continuation following antibiotic treatment and higher C-reactive protein values noted in the ALN group. Diverse genotypes were found among the E. coli isolates in both groups. Among the pathogenetic determinants examined, multivariate logistic regression analysis indicated that a papG II allele was the only significant urovirulence factor associated with ALN (P < 0.005; odds ratio, 17.16). This association was independent of the presence of VUR. CONCLUSIONS: While no specific genetic lineage was identified among the E. coli isolates studied, a papG II gene was found to be strongly associated with the cause of ALN among pediatric patients.

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