Comparison of the proliferation and differentiation ability between adult rat retinal stem cells and cerebral cortex-derived neural stem cells

I. Hsien Liu, Shih Jen Chen, Hung Hai Ku, Chung Lan Kao, Fu Ting Tsai, Wen-Ming Hsu, Chih Wen Lo, Yao Haur Kuo, Cheng Deng Kuo, Chen Hsen Lee, Shih Hwa Chiou

Research output: Contribution to journalArticle

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Abstract

Recent studies have demonstrated that retinal stem cells (RSCs) and stem cells of the central nervous system both exhibited the abilities of self-renewal, proliferation and differentiation into multilineage. In the present study, we compared the proliferation and differentiation abilities between RSCs and cerebral corticex-derived neural stem cells (CNSCs) of adult rats. Stem cells isolated from pigmented ciliary margins of eyes and cerebral cortical tissues of adult rats were cultured in 96-well plates that contained serum-free medium with epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF). In contrast to RSCs, which stopped proliferating after the 8th week, the total cell count of neurospheres in CNSCs increased twofold at the 5th week and more than fourfold at the 10th week after in vitro culture. In contrast, RSCs stopped proliferating after 8 weeks of culture. After adding 2% fetal calf serum and withdrawing EGF and bFGF from the culture medium, the percentages of nestin-positive cells (20.6 ± 2.7%), microtubule- associated-protein-2-positive neurons (33.2 ± 3.9%) and glial-fibrillary-acidic-protein-positive glial cells (51.3 ± 6.2%) in the differentiated CNSCs were significantly higher than those in the differentiated RSCs (10.2 ± 1.9, 22.3 ± 1.3 and 44.6 ± 5.1%, respectively; p < 0.05). We also found that the combination of transforming growth factor β type III with retinoic acid played an important role in the induction of CNSCs to differentiate into opsin-positive cells. Our data demonstrated that CNSCs displayed a higher ability of proliferation and retinal lineage. This report also offers an alternative protocol of cell reproduction for producing retinal cells.

Original languageEnglish
Pages (from-to)171-176
Number of pages6
JournalOphthalmologica
Volume219
Issue number3
DOIs
Publication statusPublished - May 1 2005
Externally publishedYes

Fingerprint

Neural Stem Cells
Cerebral Cortex
Stem Cells
Fibroblast Growth Factor 2
Epidermal Growth Factor
Opsins
Nestin
Microtubule-Associated Proteins
Glial Fibrillary Acidic Protein
Serum-Free Culture Media
Transforming Growth Factors
Tretinoin
Neuroglia
Reproduction
Culture Media
Central Nervous System
Cell Count
Neurons
Serum

Keywords

  • Cerebral-cortex-derived neural stem cells
  • Photoreceptors
  • Retinal stem cells
  • Transforming growth factor β type III

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems

Cite this

Comparison of the proliferation and differentiation ability between adult rat retinal stem cells and cerebral cortex-derived neural stem cells. / Liu, I. Hsien; Chen, Shih Jen; Ku, Hung Hai; Kao, Chung Lan; Tsai, Fu Ting; Hsu, Wen-Ming; Lo, Chih Wen; Kuo, Yao Haur; Kuo, Cheng Deng; Lee, Chen Hsen; Chiou, Shih Hwa.

In: Ophthalmologica, Vol. 219, No. 3, 01.05.2005, p. 171-176.

Research output: Contribution to journalArticle

Liu, IH, Chen, SJ, Ku, HH, Kao, CL, Tsai, FT, Hsu, W-M, Lo, CW, Kuo, YH, Kuo, CD, Lee, CH & Chiou, SH 2005, 'Comparison of the proliferation and differentiation ability between adult rat retinal stem cells and cerebral cortex-derived neural stem cells', Ophthalmologica, vol. 219, no. 3, pp. 171-176. https://doi.org/10.1159/000085250
Liu, I. Hsien ; Chen, Shih Jen ; Ku, Hung Hai ; Kao, Chung Lan ; Tsai, Fu Ting ; Hsu, Wen-Ming ; Lo, Chih Wen ; Kuo, Yao Haur ; Kuo, Cheng Deng ; Lee, Chen Hsen ; Chiou, Shih Hwa. / Comparison of the proliferation and differentiation ability between adult rat retinal stem cells and cerebral cortex-derived neural stem cells. In: Ophthalmologica. 2005 ; Vol. 219, No. 3. pp. 171-176.
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AB - Recent studies have demonstrated that retinal stem cells (RSCs) and stem cells of the central nervous system both exhibited the abilities of self-renewal, proliferation and differentiation into multilineage. In the present study, we compared the proliferation and differentiation abilities between RSCs and cerebral corticex-derived neural stem cells (CNSCs) of adult rats. Stem cells isolated from pigmented ciliary margins of eyes and cerebral cortical tissues of adult rats were cultured in 96-well plates that contained serum-free medium with epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF). In contrast to RSCs, which stopped proliferating after the 8th week, the total cell count of neurospheres in CNSCs increased twofold at the 5th week and more than fourfold at the 10th week after in vitro culture. In contrast, RSCs stopped proliferating after 8 weeks of culture. After adding 2% fetal calf serum and withdrawing EGF and bFGF from the culture medium, the percentages of nestin-positive cells (20.6 ± 2.7%), microtubule- associated-protein-2-positive neurons (33.2 ± 3.9%) and glial-fibrillary-acidic-protein-positive glial cells (51.3 ± 6.2%) in the differentiated CNSCs were significantly higher than those in the differentiated RSCs (10.2 ± 1.9, 22.3 ± 1.3 and 44.6 ± 5.1%, respectively; p < 0.05). We also found that the combination of transforming growth factor β type III with retinoic acid played an important role in the induction of CNSCs to differentiate into opsin-positive cells. Our data demonstrated that CNSCs displayed a higher ability of proliferation and retinal lineage. This report also offers an alternative protocol of cell reproduction for producing retinal cells.

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