Abstract
Because periodontal regeneration is vital in the modern treatment of periodontal defects, artificial membranes have become a key component for predictable outcomes. Herein a new ranking system to analyze the expression of different cytokines around regenerative membranes is introduced. Porcine dermal collagen membranes (PDCMs) and GORE-TEX® (e-PTFE) membranes were used for implantation in a Sprague Dawley rat model. Tissue samples were harvested at three time intervals (7, 10, and 14 days); then an immunohistochemical ranking process was conducted to determine the intensity of the selected Th1/Th2 cytokines [Th1: interleukin-2 (IL-2) and interferone-γ (IFN-γ); Th2: IL-4, IL-10, and IL-13]. The results show that the intensities of IL-2 cytokine in PDCM groups were slightly higher than those of e-PTFE groups but without statistical significance. The level of interferon-γ in PDCM groups was lower than that of e-PTFE groups, but also without significant differences. However, expressions of Th2 cytokines (IL-4, IL-10, and IL-13) induced by e-PTFE were generally higher than those of PDCM and control groups at all times (Mann-Whitney U test, p <0.05). In a comparison of the mean ratio of IL-2/IL-4 with the use of the Mann-Whitney U test, data for the PDCM group were generally higher than those of the e-PTFE group, with statistical significance at all time intervals (p <0.05). A descending order of the intensity ratio of IL-2/IL-4 was PDCM groups > control groups > e-PTFE groups. These findings indicate that the cytokine profiles of PDCM, in connection with the GTR technique, demonstrate a higher trend toward Th1-dominated responses and may protect against periodontal tissue destruction, as compared to the Th2-dominated responses of e-PTFE.
| Original language | English |
|---|---|
| Pages (from-to) | 75-80 |
| Number of pages | 6 |
| Journal | Journal of Biomedical Materials Research - Part B Applied Biomaterials |
| Volume | 68 |
| Issue number | 1 |
| Publication status | Published - Jan 15 2004 |
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Keywords
- e-PTFE
- PDCM
- Periodontal regeneration
- Th1/Th2 cytokines
ASJC Scopus subject areas
- Biomedical Engineering
- Biomaterials
Cite this
Comparison of Th1/Th2 Cytokine Profiles of Initial Wound Healing of Rats Induced by PDCM and e-PTFE. / Lu, Hsein Kun; Ko, Meng Tsung; Wu, Ming Fang.
In: Journal of Biomedical Materials Research - Part B Applied Biomaterials, Vol. 68, No. 1, 15.01.2004, p. 75-80.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Comparison of Th1/Th2 Cytokine Profiles of Initial Wound Healing of Rats Induced by PDCM and e-PTFE
AU - Lu, Hsein Kun
AU - Ko, Meng Tsung
AU - Wu, Ming Fang
PY - 2004/1/15
Y1 - 2004/1/15
N2 - Because periodontal regeneration is vital in the modern treatment of periodontal defects, artificial membranes have become a key component for predictable outcomes. Herein a new ranking system to analyze the expression of different cytokines around regenerative membranes is introduced. Porcine dermal collagen membranes (PDCMs) and GORE-TEX® (e-PTFE) membranes were used for implantation in a Sprague Dawley rat model. Tissue samples were harvested at three time intervals (7, 10, and 14 days); then an immunohistochemical ranking process was conducted to determine the intensity of the selected Th1/Th2 cytokines [Th1: interleukin-2 (IL-2) and interferone-γ (IFN-γ); Th2: IL-4, IL-10, and IL-13]. The results show that the intensities of IL-2 cytokine in PDCM groups were slightly higher than those of e-PTFE groups but without statistical significance. The level of interferon-γ in PDCM groups was lower than that of e-PTFE groups, but also without significant differences. However, expressions of Th2 cytokines (IL-4, IL-10, and IL-13) induced by e-PTFE were generally higher than those of PDCM and control groups at all times (Mann-Whitney U test, p <0.05). In a comparison of the mean ratio of IL-2/IL-4 with the use of the Mann-Whitney U test, data for the PDCM group were generally higher than those of the e-PTFE group, with statistical significance at all time intervals (p <0.05). A descending order of the intensity ratio of IL-2/IL-4 was PDCM groups > control groups > e-PTFE groups. These findings indicate that the cytokine profiles of PDCM, in connection with the GTR technique, demonstrate a higher trend toward Th1-dominated responses and may protect against periodontal tissue destruction, as compared to the Th2-dominated responses of e-PTFE.
AB - Because periodontal regeneration is vital in the modern treatment of periodontal defects, artificial membranes have become a key component for predictable outcomes. Herein a new ranking system to analyze the expression of different cytokines around regenerative membranes is introduced. Porcine dermal collagen membranes (PDCMs) and GORE-TEX® (e-PTFE) membranes were used for implantation in a Sprague Dawley rat model. Tissue samples were harvested at three time intervals (7, 10, and 14 days); then an immunohistochemical ranking process was conducted to determine the intensity of the selected Th1/Th2 cytokines [Th1: interleukin-2 (IL-2) and interferone-γ (IFN-γ); Th2: IL-4, IL-10, and IL-13]. The results show that the intensities of IL-2 cytokine in PDCM groups were slightly higher than those of e-PTFE groups but without statistical significance. The level of interferon-γ in PDCM groups was lower than that of e-PTFE groups, but also without significant differences. However, expressions of Th2 cytokines (IL-4, IL-10, and IL-13) induced by e-PTFE were generally higher than those of PDCM and control groups at all times (Mann-Whitney U test, p <0.05). In a comparison of the mean ratio of IL-2/IL-4 with the use of the Mann-Whitney U test, data for the PDCM group were generally higher than those of the e-PTFE group, with statistical significance at all time intervals (p <0.05). A descending order of the intensity ratio of IL-2/IL-4 was PDCM groups > control groups > e-PTFE groups. These findings indicate that the cytokine profiles of PDCM, in connection with the GTR technique, demonstrate a higher trend toward Th1-dominated responses and may protect against periodontal tissue destruction, as compared to the Th2-dominated responses of e-PTFE.
KW - e-PTFE
KW - PDCM
KW - Periodontal regeneration
KW - Th1/Th2 cytokines
UR - http://www.scopus.com/inward/record.url?scp=0347986708&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0347986708&partnerID=8YFLogxK
M3 - Article
C2 - 14689499
AN - SCOPUS:0347986708
VL - 68
SP - 75
EP - 80
JO - Journal of Biomedical Materials Research - Part B Applied Biomaterials
JF - Journal of Biomedical Materials Research - Part B Applied Biomaterials
SN - 1552-4973
IS - 1
ER -