Comparison of Steroid and Botulinum Toxin Type A Monotherapy with Combination Therapy for Treating Human Hypertrophic Scars in an Animal Model

Hung Chang Chen, Cheng I. Yen, Shih Yi Yang, Cheng Jen Chang, Jui Yung Yang, Shu Yin Chang, Shiow Shuh Chuang, Yen Chang Hsiao

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Background: The authors evaluated the efficacy of a combined regimen of botulinum toxin type A (Botox) and a steroid (triamcinolone acetonide) for treating hypertrophic scars in comparison with the treatment with each drug alone. Methods: Twenty excised human hypertrophic scar fragments obtained from surgically treated burn patients were divided into four groups: Negative control (group A), triamcinolone alone (group B), Botox alone (group C), and a combination of triamcinolone and Botox (group D). These specimens were implanted into the backs of nude mice after intralesional injection from each group and were observed for 4 weeks. In total, 12 mice and 48 scars were studied. After 4 weeks, the hypertrophic scars were removed from the backs. The authors compared the scar weights, decorin staining, and the Cell Counting Kit-8 assay to evaluate treatment efficacy. Results: Significant differences in scar weight reduction were observed among the four groups (group A, 10 percent; group B, 17 percent; group C, 23 percent; and group D, 30 percent; p < 0.05). Treatment groups (groups B, C, and D) showed strong decorin staining. Significant differences in reduction of fibroblast proliferation were observed among the four groups (group A, 0.58; group B, 0.44; group C, 0.21; and group D, 0.08; p < 0.05). Botox or triamcinolone intralesional monotherapy showed significant therapeutic efficacy compared with the control group. The combined therapy further exhibited a significant therapeutic effect compared with monotherapy. Conclusion: This study indicates the potential of Botox and triamcinolone when combined for intralesional therapy in treating hypertrophic scars.

Original languageEnglish
Pages (from-to)43e-49e
JournalPlastic and Reconstructive Surgery
Volume140
Issue number1
DOIs
Publication statusPublished - Jul 1 2017
Externally publishedYes

Fingerprint

Hypertrophic Cicatrix
Type A Botulinum Toxins
Triamcinolone
Animal Models
Steroids
Decorin
Cicatrix
Intralesional Injections
Staining and Labeling
Therapeutics
Triamcinolone Acetonide
Control Groups
Therapeutic Uses
Nude Mice
Weight Loss
Fibroblasts
onabotulinumtoxinA
Weights and Measures
Pharmaceutical Preparations

ASJC Scopus subject areas

  • Surgery

Cite this

Comparison of Steroid and Botulinum Toxin Type A Monotherapy with Combination Therapy for Treating Human Hypertrophic Scars in an Animal Model. / Chen, Hung Chang; Yen, Cheng I.; Yang, Shih Yi; Chang, Cheng Jen; Yang, Jui Yung; Chang, Shu Yin; Chuang, Shiow Shuh; Hsiao, Yen Chang.

In: Plastic and Reconstructive Surgery, Vol. 140, No. 1, 01.07.2017, p. 43e-49e.

Research output: Contribution to journalArticle

Chen, Hung Chang ; Yen, Cheng I. ; Yang, Shih Yi ; Chang, Cheng Jen ; Yang, Jui Yung ; Chang, Shu Yin ; Chuang, Shiow Shuh ; Hsiao, Yen Chang. / Comparison of Steroid and Botulinum Toxin Type A Monotherapy with Combination Therapy for Treating Human Hypertrophic Scars in an Animal Model. In: Plastic and Reconstructive Surgery. 2017 ; Vol. 140, No. 1. pp. 43e-49e.
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T1 - Comparison of Steroid and Botulinum Toxin Type A Monotherapy with Combination Therapy for Treating Human Hypertrophic Scars in an Animal Model

AU - Chen, Hung Chang

AU - Yen, Cheng I.

AU - Yang, Shih Yi

AU - Chang, Cheng Jen

AU - Yang, Jui Yung

AU - Chang, Shu Yin

AU - Chuang, Shiow Shuh

AU - Hsiao, Yen Chang

PY - 2017/7/1

Y1 - 2017/7/1

N2 - Background: The authors evaluated the efficacy of a combined regimen of botulinum toxin type A (Botox) and a steroid (triamcinolone acetonide) for treating hypertrophic scars in comparison with the treatment with each drug alone. Methods: Twenty excised human hypertrophic scar fragments obtained from surgically treated burn patients were divided into four groups: Negative control (group A), triamcinolone alone (group B), Botox alone (group C), and a combination of triamcinolone and Botox (group D). These specimens were implanted into the backs of nude mice after intralesional injection from each group and were observed for 4 weeks. In total, 12 mice and 48 scars were studied. After 4 weeks, the hypertrophic scars were removed from the backs. The authors compared the scar weights, decorin staining, and the Cell Counting Kit-8 assay to evaluate treatment efficacy. Results: Significant differences in scar weight reduction were observed among the four groups (group A, 10 percent; group B, 17 percent; group C, 23 percent; and group D, 30 percent; p < 0.05). Treatment groups (groups B, C, and D) showed strong decorin staining. Significant differences in reduction of fibroblast proliferation were observed among the four groups (group A, 0.58; group B, 0.44; group C, 0.21; and group D, 0.08; p < 0.05). Botox or triamcinolone intralesional monotherapy showed significant therapeutic efficacy compared with the control group. The combined therapy further exhibited a significant therapeutic effect compared with monotherapy. Conclusion: This study indicates the potential of Botox and triamcinolone when combined for intralesional therapy in treating hypertrophic scars.

AB - Background: The authors evaluated the efficacy of a combined regimen of botulinum toxin type A (Botox) and a steroid (triamcinolone acetonide) for treating hypertrophic scars in comparison with the treatment with each drug alone. Methods: Twenty excised human hypertrophic scar fragments obtained from surgically treated burn patients were divided into four groups: Negative control (group A), triamcinolone alone (group B), Botox alone (group C), and a combination of triamcinolone and Botox (group D). These specimens were implanted into the backs of nude mice after intralesional injection from each group and were observed for 4 weeks. In total, 12 mice and 48 scars were studied. After 4 weeks, the hypertrophic scars were removed from the backs. The authors compared the scar weights, decorin staining, and the Cell Counting Kit-8 assay to evaluate treatment efficacy. Results: Significant differences in scar weight reduction were observed among the four groups (group A, 10 percent; group B, 17 percent; group C, 23 percent; and group D, 30 percent; p < 0.05). Treatment groups (groups B, C, and D) showed strong decorin staining. Significant differences in reduction of fibroblast proliferation were observed among the four groups (group A, 0.58; group B, 0.44; group C, 0.21; and group D, 0.08; p < 0.05). Botox or triamcinolone intralesional monotherapy showed significant therapeutic efficacy compared with the control group. The combined therapy further exhibited a significant therapeutic effect compared with monotherapy. Conclusion: This study indicates the potential of Botox and triamcinolone when combined for intralesional therapy in treating hypertrophic scars.

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