Comparison of Quinn's Advantage fertilization medium and tissue culture medium 199 for invitro maturation of oocytes

Yu Hung Lin, Jiann-Loung Hwang, Lee Wen Huang, Kok Min Seow, Bih Chwen Hsieh, Chii Ruey Tzeng

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Objective: The purpose of the study was to compare the Quinn's Advantage fertilization medium (Q1) and the tissue culture medium 199 (TCM199) for invitro maturation (IVM) of oocytes and ammonium production during IVM. Materials and methods: The immature murine oocytes were randomly added into Q1 and TCM199. Ammonium concentrations were measured at the start and after 18hours of IVM, and the mature oocytes were fertilized and cultured into blastocysts. The blastocysts were then stained for inner cell mass (ICM) and trophectoderm. Results: The maturation rate was higher in Q1 than in TCM199 (85.7% vs. 76.6%, p=0.024). The fertilization and blastocyst rates were slightly higher in Q1, but not significant. Differential staining of the blastocysts showed slightly higher ICM ratio in the blastocysts derived from Q1. Mean ammonium concentrations in Q1 and TCM199 at Time 0 were 184.9 and 339.2μg/dL, respectively (p=0.05), and after 18hours of IVM were 268.7 and 443.6μg/dL, respectively (p=0.045). Addition of ammonium chloride into Q1 adversely affects IVM. Conclusion: Q1 is superior to TCM199 in terms of oocyte maturation, which may be due to lower ammonium concentration.

Original languageEnglish
Pages (from-to)17-20
Number of pages4
JournalTaiwanese Journal of Obstetrics and Gynecology
Volume53
Issue number1
DOIs
Publication statusPublished - 2014

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Blastocyst
Fertilization
Oocytes
Culture Media
Ammonium Compounds
Ammonium Chloride
Staining and Labeling

Keywords

  • Ammonium
  • Blastocyst
  • Invitro maturation

ASJC Scopus subject areas

  • Obstetrics and Gynaecology
  • Medicine(all)

Cite this

Comparison of Quinn's Advantage fertilization medium and tissue culture medium 199 for invitro maturation of oocytes. / Lin, Yu Hung; Hwang, Jiann-Loung; Huang, Lee Wen; Seow, Kok Min; Hsieh, Bih Chwen; Tzeng, Chii Ruey.

In: Taiwanese Journal of Obstetrics and Gynecology, Vol. 53, No. 1, 2014, p. 17-20.

Research output: Contribution to journalArticle

Lin, Yu Hung ; Hwang, Jiann-Loung ; Huang, Lee Wen ; Seow, Kok Min ; Hsieh, Bih Chwen ; Tzeng, Chii Ruey. / Comparison of Quinn's Advantage fertilization medium and tissue culture medium 199 for invitro maturation of oocytes. In: Taiwanese Journal of Obstetrics and Gynecology. 2014 ; Vol. 53, No. 1. pp. 17-20.
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AB - Objective: The purpose of the study was to compare the Quinn's Advantage fertilization medium (Q1) and the tissue culture medium 199 (TCM199) for invitro maturation (IVM) of oocytes and ammonium production during IVM. Materials and methods: The immature murine oocytes were randomly added into Q1 and TCM199. Ammonium concentrations were measured at the start and after 18hours of IVM, and the mature oocytes were fertilized and cultured into blastocysts. The blastocysts were then stained for inner cell mass (ICM) and trophectoderm. Results: The maturation rate was higher in Q1 than in TCM199 (85.7% vs. 76.6%, p=0.024). The fertilization and blastocyst rates were slightly higher in Q1, but not significant. Differential staining of the blastocysts showed slightly higher ICM ratio in the blastocysts derived from Q1. Mean ammonium concentrations in Q1 and TCM199 at Time 0 were 184.9 and 339.2μg/dL, respectively (p=0.05), and after 18hours of IVM were 268.7 and 443.6μg/dL, respectively (p=0.045). Addition of ammonium chloride into Q1 adversely affects IVM. Conclusion: Q1 is superior to TCM199 in terms of oocyte maturation, which may be due to lower ammonium concentration.

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