Comparison of protein phosphatase inhibition activities and mouse toxicities of microcystins

Yi Min Chen, Zong-Huei Li, Shyh Jye Lee, Hsien Bin Huang, Rang Huang, Hong Nong Chou

Research output: Contribution to journalArticle

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Abstract

Eight naturally purified microcystins (MCs), including MC-LR, -FR, -WR, -RR, [d-Asp3]MC-FR, -WR, -RR, and [Dha7]MC-RR were utilized to determine the effects of amino acid substitutions and modifications on MC-induced protein phosphatase inhibition activity and mouse toxicity. Catalytic subunits of protein phosphatase 1 (PP-1) and 2A (PP-2A) were purified and subjected to the inhibition assays, and intraperitoneal injection was used to administer MCs into mice for the toxicity assay. It is found that the replacement of the non-polar amino acid l-leucine at the second position of these heptacyclic peptide toxins by a polar l-arginine reduces their mouse toxicities and inhibitory activities against PP-1 and PP-2A to different extends. Demethylation of methyldehydroalanine (Mdha) at the seventh amino acid of MC-RR exhibits the least mouse toxicity and phosphatase inhibition. The loss of a methyl group on the common methylaspartic acid (MeAsp) at the third position of MC-FR, -WR, and -RR does not alter their toxicity levels, but dominantly reduces their activities in PP-1 inhibition compared to other substitutions or modifications. This suggests that the methyl group on MeAsp is also important for MCs inhibition. However, such a tendency is not observed for PP-2A. By comparing the LD50 values of the mouse toxicity assay and IC50 values of the PP-1 and PP-2A inhibition assay of eight MCs using linear regression, it is evident that the MC-induced toxicity is much more related to the inhibition of PP-2A than PP-1, which suggests that PP-2A inhibition may play a major role in the MC-induced mouse toxicity.

Original languageEnglish
Pages (from-to)742-746
Number of pages5
JournalToxicon
Volume47
Issue number7
DOIs
Publication statusPublished - Jun 1 2006

Fingerprint

Microcystins
Phosphoprotein Phosphatases
Toxicity
Protein Phosphatase 1
Assays
Amino Acids
Substitution reactions
Protein Phosphatase 2
Acids
Lethal Dose 50
Amino Acid Substitution
Intraperitoneal Injections
Phosphoric Monoester Hydrolases
Linear regression
Leucine
Inhibitory Concentration 50
Arginine
Linear Models

Keywords

  • Microcystin (MC)
  • Mouse assay
  • Protein phosphatase 1 (PP-1)
  • Protein phosphatase 2A (PP-2A)

ASJC Scopus subject areas

  • Toxicology

Cite this

Chen, Y. M., Li, Z-H., Lee, S. J., Huang, H. B., Huang, R., & Chou, H. N. (2006). Comparison of protein phosphatase inhibition activities and mouse toxicities of microcystins. Toxicon, 47(7), 742-746. https://doi.org/10.1016/j.toxicon.2006.01.026

Comparison of protein phosphatase inhibition activities and mouse toxicities of microcystins. / Chen, Yi Min; Li, Zong-Huei; Lee, Shyh Jye; Huang, Hsien Bin; Huang, Rang; Chou, Hong Nong.

In: Toxicon, Vol. 47, No. 7, 01.06.2006, p. 742-746.

Research output: Contribution to journalArticle

Chen, YM, Li, Z-H, Lee, SJ, Huang, HB, Huang, R & Chou, HN 2006, 'Comparison of protein phosphatase inhibition activities and mouse toxicities of microcystins', Toxicon, vol. 47, no. 7, pp. 742-746. https://doi.org/10.1016/j.toxicon.2006.01.026
Chen, Yi Min ; Li, Zong-Huei ; Lee, Shyh Jye ; Huang, Hsien Bin ; Huang, Rang ; Chou, Hong Nong. / Comparison of protein phosphatase inhibition activities and mouse toxicities of microcystins. In: Toxicon. 2006 ; Vol. 47, No. 7. pp. 742-746.
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