Comparison of prevalence of virulence factors for Klebsiella pneumoniae liver abscesses between isolates with capsular K1/K2 and non-K1/K2 serotypes

Wen Liang Yu, Wen Chien Ko, Kuo-Chen Cheng, Ching Chien Lee, Chien-Cheng Lai, Yin Ching Chuang

Research output: Contribution to journalArticle

140 Citations (Scopus)

Abstract

Hypermucoviscosity, rmpA (regulator of mucoid phenotype), aerobactin (an iron siderophore), kfu (an iron uptake system), allS (associated with allantoin metabolism), and K1/K2 capsules are important virulence determinants in Klebsiella pneumoniae for liver abscesses. We determined the prevalence of these virulence factors of 50 nonrepeat K. pneumoniae isolates recovered from patients with primary liver abscesses who were treated at 2 medical centers in Taiwan. Virulence genes were surveyed by polymerase chain reaction analysis. The prevalence of hypermucoviscosity phenotype, plasmid-born rmpA, aerobactin, kfu, and allS genes revealed 96%, 100%, 100%, 100%, and 100% in 26 capsular K1 isolates; 90%, 100%, 100%, 0%, and 0% in 10 K2 isolates; and 79%, 86%, 93%, 50%, and 0% in 14 non-K1/K2 isolates; respectively. When injected into mice intraperitoneally, regardless of any capsule K serotype, K. pneumoniae isolates with hypermucoviscosity phenotype as well as presence of rmpA and aerobactin genes exhibited high virulence for mouse lethality (LD50, 2 CFU). Without significant difference in the prevalence of expressing hypermucoviscosity phenotype and carriage of rmpA and aerobactin genes, these virulent non-K1/K2 isolates are as capable as K1/K2 isolates of causing primary liver abscesses.

Original languageEnglish
Pages (from-to)1-6
Number of pages6
JournalDiagnostic Microbiology and Infectious Disease
Volume62
Issue number1
DOIs
Publication statusPublished - Sep 2008

Fingerprint

Liver Abscess
Klebsiella pneumoniae
Virulence Factors
Phenotype
Virulence
Genes
Capsules
Iron
Allantoin
Siderophores
Lethal Dose 50
Taiwan
Plasmids
Polymerase Chain Reaction
Serogroup
aerobactin

Keywords

  • Hypermucoviscosity
  • Klebsiella pneumoniae
  • Liver abscess
  • rmpA gene
  • Virulence

ASJC Scopus subject areas

  • Infectious Diseases
  • Immunology and Allergy
  • Virology
  • Parasitology
  • Microbiology
  • Immunology
  • Applied Microbiology and Biotechnology

Cite this

Comparison of prevalence of virulence factors for Klebsiella pneumoniae liver abscesses between isolates with capsular K1/K2 and non-K1/K2 serotypes. / Yu, Wen Liang; Ko, Wen Chien; Cheng, Kuo-Chen; Lee, Ching Chien; Lai, Chien-Cheng; Chuang, Yin Ching.

In: Diagnostic Microbiology and Infectious Disease, Vol. 62, No. 1, 09.2008, p. 1-6.

Research output: Contribution to journalArticle

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