Comparison of organic and inorganic germanium compounds in cellular radiosensitivity and preparation of germanium nanoparticles as a radiosensitizer

Ming Hsing Lin, Tzu Sheng Hsu, Pei Ming Yang, Meng Yen Tsai, Tsong Pyng Perng, Lih Yuan Lin

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Purpose: The aim of this work is to compare the radiosensitizing effect between organic and inorganic germanium compounds and to investigate whether nanometer-sized germanium particles can act as radiosensitizers. Materials and methods: Bis (2-carboxyethylgermanium) sesquioxide (Ge-132), germanium oxide (GeO2) and germanium nanoparticles were used in this study. Cell viability was determined by clonogenic survival assay. Cellular DNA damage was evaluated by alkaline comet assay, confocal microscopy and the cellular level of phospho-histone H2AX (-H2AX). Results: Nanometer-sized germanium particles were fabricated. They have a similar radiosensitizing effect as that of GeO2. Conversely, Ge-132 did not enhance the radiosensitivity of cells. Comet assay was employed to evaluate the level of DNA damage and confirmed that inorganic germanium compounds enhanced cellular radiosensitivity. Notably, the comet assay indicated that the nanoparticle itself caused a higher level of DNA damage. The possibility that germanium nanoparticles per se caused DNA damage was ruled out when the cellular level of -H2AX was examined. Conclusions: We demonstrated that inorganic but not organic germanium compounds exerted radiosensitizing effect in cells. Nanometer-sized germanium particles were fabricated and were able to enhance the radiosensitivity of cells. Confounding effect may occur when comet assay is used to estimate the level of DNA damage in the presence of germanium nanoparticles.

Original languageEnglish
Pages (from-to)214-226
Number of pages13
JournalInternational Journal of Radiation Biology
Volume85
Issue number3
DOIs
Publication statusPublished - Mar 2009
Externally publishedYes

Fingerprint

Germanium
Radiation Tolerance
Nanoparticles
Comet Assay
DNA Damage
Radiation-Sensitizing Agents
Confocal Microscopy
Histones
Cell Survival

Keywords

  • Cell growth
  • CHO cell
  • Comet assay
  • Ge-132
  • Germanium oxide
  • Nanoparticle
  • Radiosensitizing effect

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology

Cite this

Comparison of organic and inorganic germanium compounds in cellular radiosensitivity and preparation of germanium nanoparticles as a radiosensitizer. / Lin, Ming Hsing; Hsu, Tzu Sheng; Yang, Pei Ming; Tsai, Meng Yen; Perng, Tsong Pyng; Lin, Lih Yuan.

In: International Journal of Radiation Biology, Vol. 85, No. 3, 03.2009, p. 214-226.

Research output: Contribution to journalArticle

@article{ecce9c8c1a444c88bb171451d3f2098f,
title = "Comparison of organic and inorganic germanium compounds in cellular radiosensitivity and preparation of germanium nanoparticles as a radiosensitizer",
abstract = "Purpose: The aim of this work is to compare the radiosensitizing effect between organic and inorganic germanium compounds and to investigate whether nanometer-sized germanium particles can act as radiosensitizers. Materials and methods: Bis (2-carboxyethylgermanium) sesquioxide (Ge-132), germanium oxide (GeO2) and germanium nanoparticles were used in this study. Cell viability was determined by clonogenic survival assay. Cellular DNA damage was evaluated by alkaline comet assay, confocal microscopy and the cellular level of phospho-histone H2AX (-H2AX). Results: Nanometer-sized germanium particles were fabricated. They have a similar radiosensitizing effect as that of GeO2. Conversely, Ge-132 did not enhance the radiosensitivity of cells. Comet assay was employed to evaluate the level of DNA damage and confirmed that inorganic germanium compounds enhanced cellular radiosensitivity. Notably, the comet assay indicated that the nanoparticle itself caused a higher level of DNA damage. The possibility that germanium nanoparticles per se caused DNA damage was ruled out when the cellular level of -H2AX was examined. Conclusions: We demonstrated that inorganic but not organic germanium compounds exerted radiosensitizing effect in cells. Nanometer-sized germanium particles were fabricated and were able to enhance the radiosensitivity of cells. Confounding effect may occur when comet assay is used to estimate the level of DNA damage in the presence of germanium nanoparticles.",
keywords = "Cell growth, CHO cell, Comet assay, Ge-132, Germanium oxide, Nanoparticle, Radiosensitizing effect",
author = "Lin, {Ming Hsing} and Hsu, {Tzu Sheng} and Yang, {Pei Ming} and Tsai, {Meng Yen} and Perng, {Tsong Pyng} and Lin, {Lih Yuan}",
year = "2009",
month = "3",
doi = "10.1080/09553000902748583",
language = "English",
volume = "85",
pages = "214--226",
journal = "International Journal of Radiation Biology",
issn = "0955-3002",
publisher = "Informa Healthcare",
number = "3",

}

TY - JOUR

T1 - Comparison of organic and inorganic germanium compounds in cellular radiosensitivity and preparation of germanium nanoparticles as a radiosensitizer

AU - Lin, Ming Hsing

AU - Hsu, Tzu Sheng

AU - Yang, Pei Ming

AU - Tsai, Meng Yen

AU - Perng, Tsong Pyng

AU - Lin, Lih Yuan

PY - 2009/3

Y1 - 2009/3

N2 - Purpose: The aim of this work is to compare the radiosensitizing effect between organic and inorganic germanium compounds and to investigate whether nanometer-sized germanium particles can act as radiosensitizers. Materials and methods: Bis (2-carboxyethylgermanium) sesquioxide (Ge-132), germanium oxide (GeO2) and germanium nanoparticles were used in this study. Cell viability was determined by clonogenic survival assay. Cellular DNA damage was evaluated by alkaline comet assay, confocal microscopy and the cellular level of phospho-histone H2AX (-H2AX). Results: Nanometer-sized germanium particles were fabricated. They have a similar radiosensitizing effect as that of GeO2. Conversely, Ge-132 did not enhance the radiosensitivity of cells. Comet assay was employed to evaluate the level of DNA damage and confirmed that inorganic germanium compounds enhanced cellular radiosensitivity. Notably, the comet assay indicated that the nanoparticle itself caused a higher level of DNA damage. The possibility that germanium nanoparticles per se caused DNA damage was ruled out when the cellular level of -H2AX was examined. Conclusions: We demonstrated that inorganic but not organic germanium compounds exerted radiosensitizing effect in cells. Nanometer-sized germanium particles were fabricated and were able to enhance the radiosensitivity of cells. Confounding effect may occur when comet assay is used to estimate the level of DNA damage in the presence of germanium nanoparticles.

AB - Purpose: The aim of this work is to compare the radiosensitizing effect between organic and inorganic germanium compounds and to investigate whether nanometer-sized germanium particles can act as radiosensitizers. Materials and methods: Bis (2-carboxyethylgermanium) sesquioxide (Ge-132), germanium oxide (GeO2) and germanium nanoparticles were used in this study. Cell viability was determined by clonogenic survival assay. Cellular DNA damage was evaluated by alkaline comet assay, confocal microscopy and the cellular level of phospho-histone H2AX (-H2AX). Results: Nanometer-sized germanium particles were fabricated. They have a similar radiosensitizing effect as that of GeO2. Conversely, Ge-132 did not enhance the radiosensitivity of cells. Comet assay was employed to evaluate the level of DNA damage and confirmed that inorganic germanium compounds enhanced cellular radiosensitivity. Notably, the comet assay indicated that the nanoparticle itself caused a higher level of DNA damage. The possibility that germanium nanoparticles per se caused DNA damage was ruled out when the cellular level of -H2AX was examined. Conclusions: We demonstrated that inorganic but not organic germanium compounds exerted radiosensitizing effect in cells. Nanometer-sized germanium particles were fabricated and were able to enhance the radiosensitivity of cells. Confounding effect may occur when comet assay is used to estimate the level of DNA damage in the presence of germanium nanoparticles.

KW - Cell growth

KW - CHO cell

KW - Comet assay

KW - Ge-132

KW - Germanium oxide

KW - Nanoparticle

KW - Radiosensitizing effect

UR - http://www.scopus.com/inward/record.url?scp=67449108219&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=67449108219&partnerID=8YFLogxK

U2 - 10.1080/09553000902748583

DO - 10.1080/09553000902748583

M3 - Article

VL - 85

SP - 214

EP - 226

JO - International Journal of Radiation Biology

JF - International Journal of Radiation Biology

SN - 0955-3002

IS - 3

ER -