Comparison of high purity factor IX concentrates and a prothrombin complex concentrate in a canine model of thrombogenicity

I. R. MacGregor, J. M. Ferguson, L. F. McLaughlin, T. Burnouf, C. V. Prowse

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

A non-stasis canine model of thrombogenicity has been used to evaluate batches of high purity factor IX concentrates from 4 manufacturers and a conventional prothrombin complex concentrate (PCC). Platelets, activated partial thromboplastin time (APTT), fibrinogen, fibrin(ogen) degradation products and fibrinopeptide A (FPA) were monitored before and after infusion of concentrate. Changes in FPA were found to be the most sensitive and reproducible indicator of thrombogenicity after infusion of batches of the PCC at doses of between 60 and 180 IU/kg, with a dose related delayed increase in FPA occurring. Total FPA generated after 100-120 IU/kg of 3 batches of PCC over the 3 h time course was 9-12 times that generated after albumin infusion. In contrast the amounts of FPA generated after 200 IU/kg of the 4 high purity factor IX products were in all cases similar to albumin infusion. It was noted that some batches of high purity concentrates had short NAPTTs indicating that current in vitro tests for potential thrombogenicity may be misleading in predicting the effects of these concentrates in vivo.

Original languageEnglish
Pages (from-to)609-613
Number of pages5
JournalThrombosis and Haemostasis
Volume66
Issue number5
Publication statusPublished - 1991
Externally publishedYes

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Fibrinopeptide A
Canidae
Albumins
Fibrin Fibrinogen Degradation Products
Factor IX
Partial Thromboplastin Time
Blood Platelets
prothrombin complex concentrates

ASJC Scopus subject areas

  • Hematology

Cite this

Comparison of high purity factor IX concentrates and a prothrombin complex concentrate in a canine model of thrombogenicity. / MacGregor, I. R.; Ferguson, J. M.; McLaughlin, L. F.; Burnouf, T.; Prowse, C. V.

In: Thrombosis and Haemostasis, Vol. 66, No. 5, 1991, p. 609-613.

Research output: Contribution to journalArticle

MacGregor, I. R. ; Ferguson, J. M. ; McLaughlin, L. F. ; Burnouf, T. ; Prowse, C. V. / Comparison of high purity factor IX concentrates and a prothrombin complex concentrate in a canine model of thrombogenicity. In: Thrombosis and Haemostasis. 1991 ; Vol. 66, No. 5. pp. 609-613.
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