Comparison of glutathione peroxidase-3 protein expression and enzyme bioactivity in normal subjects and patients with sepsis

Wei Ju Lee, Yen Ling Chen, Yi Wen Chu, Du Shieng Chien

Research output: Contribution to journalArticle

Abstract

Background: Serum glutathione peroxidase-3 (GPx-3) is known as a key selenoprotein with antioxidant properties. GPx-3 deficiency has been associated with sepsis. The objectives of this study are (1) to compare the GPx-3 protein concentrations and GPx-3 bioactivity in normal healthy subjects and septic patients, and (2) to evaluate the relationship between GPx-3 bioactivity and its protein concentration. Methods: Serum samples were collected from 50 normal healthy subjects and 70 septic patients. The reliable bioanalytical methods for GPx-3 protein concentration and bioactivity in human serum were developed and validated. Analyses of GPx-3 bioactivity and GPx-3 protein concentration were then performed. Results: Geometric mean GPx-3 bioactivity was 78.13. U/l for patients with sepsis, significantly lower than normal subjects with 108.21. U/l (p <. 0.0001). Similarly, the GPx-3 protein concentration was significantly lower in patients with sepsis than in normal subjects, with the mean GPx-3 value of 0.78 vs 3.10. μg/ml, respectively (p <. 0.0001). A positive correlation was observed between the GPx-3 bioactivity and its corresponding protein concentration in septic serum samples (R = 0.74, p <. 0.0001), regardless of gender or age difference. Conclusion: These findings suggest that the decrease in GPx-3 bioactivity observed in the septic patients was resulted from the significant sepsis-related decline of GPx-3 protein concentrations.

Original languageEnglish
JournalClinica Chimica Acta
DOIs
Publication statusPublished - 2019
Externally publishedYes

Fingerprint

Glutathione Peroxidase
Bioactivity
Sepsis
Enzymes
Proteins
Serum
Healthy Volunteers
Selenoproteins
Antioxidants

Keywords

  • Biomarker
  • GPx-3 bioactivity
  • GPx-3 protein concentration
  • Selenium
  • Sepsis

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Biochemistry, medical

Cite this

Comparison of glutathione peroxidase-3 protein expression and enzyme bioactivity in normal subjects and patients with sepsis. / Lee, Wei Ju; Chen, Yen Ling; Chu, Yi Wen; Chien, Du Shieng.

In: Clinica Chimica Acta, 2019.

Research output: Contribution to journalArticle

@article{77fecf07eb97467abb1ece843725fda6,
title = "Comparison of glutathione peroxidase-3 protein expression and enzyme bioactivity in normal subjects and patients with sepsis",
abstract = "Background: Serum glutathione peroxidase-3 (GPx-3) is known as a key selenoprotein with antioxidant properties. GPx-3 deficiency has been associated with sepsis. The objectives of this study are (1) to compare the GPx-3 protein concentrations and GPx-3 bioactivity in normal healthy subjects and septic patients, and (2) to evaluate the relationship between GPx-3 bioactivity and its protein concentration. Methods: Serum samples were collected from 50 normal healthy subjects and 70 septic patients. The reliable bioanalytical methods for GPx-3 protein concentration and bioactivity in human serum were developed and validated. Analyses of GPx-3 bioactivity and GPx-3 protein concentration were then performed. Results: Geometric mean GPx-3 bioactivity was 78.13. U/l for patients with sepsis, significantly lower than normal subjects with 108.21. U/l (p <. 0.0001). Similarly, the GPx-3 protein concentration was significantly lower in patients with sepsis than in normal subjects, with the mean GPx-3 value of 0.78 vs 3.10. μg/ml, respectively (p <. 0.0001). A positive correlation was observed between the GPx-3 bioactivity and its corresponding protein concentration in septic serum samples (R = 0.74, p <. 0.0001), regardless of gender or age difference. Conclusion: These findings suggest that the decrease in GPx-3 bioactivity observed in the septic patients was resulted from the significant sepsis-related decline of GPx-3 protein concentrations.",
keywords = "Biomarker, GPx-3 bioactivity, GPx-3 protein concentration, Selenium, Sepsis",
author = "Lee, {Wei Ju} and Chen, {Yen Ling} and Chu, {Yi Wen} and Chien, {Du Shieng}",
year = "2019",
doi = "10.1016/j.cca.2017.10.031",
language = "English",
journal = "Clinica Chimica Acta",
issn = "0009-8981",
publisher = "Elsevier",

}

TY - JOUR

T1 - Comparison of glutathione peroxidase-3 protein expression and enzyme bioactivity in normal subjects and patients with sepsis

AU - Lee, Wei Ju

AU - Chen, Yen Ling

AU - Chu, Yi Wen

AU - Chien, Du Shieng

PY - 2019

Y1 - 2019

N2 - Background: Serum glutathione peroxidase-3 (GPx-3) is known as a key selenoprotein with antioxidant properties. GPx-3 deficiency has been associated with sepsis. The objectives of this study are (1) to compare the GPx-3 protein concentrations and GPx-3 bioactivity in normal healthy subjects and septic patients, and (2) to evaluate the relationship between GPx-3 bioactivity and its protein concentration. Methods: Serum samples were collected from 50 normal healthy subjects and 70 septic patients. The reliable bioanalytical methods for GPx-3 protein concentration and bioactivity in human serum were developed and validated. Analyses of GPx-3 bioactivity and GPx-3 protein concentration were then performed. Results: Geometric mean GPx-3 bioactivity was 78.13. U/l for patients with sepsis, significantly lower than normal subjects with 108.21. U/l (p <. 0.0001). Similarly, the GPx-3 protein concentration was significantly lower in patients with sepsis than in normal subjects, with the mean GPx-3 value of 0.78 vs 3.10. μg/ml, respectively (p <. 0.0001). A positive correlation was observed between the GPx-3 bioactivity and its corresponding protein concentration in septic serum samples (R = 0.74, p <. 0.0001), regardless of gender or age difference. Conclusion: These findings suggest that the decrease in GPx-3 bioactivity observed in the septic patients was resulted from the significant sepsis-related decline of GPx-3 protein concentrations.

AB - Background: Serum glutathione peroxidase-3 (GPx-3) is known as a key selenoprotein with antioxidant properties. GPx-3 deficiency has been associated with sepsis. The objectives of this study are (1) to compare the GPx-3 protein concentrations and GPx-3 bioactivity in normal healthy subjects and septic patients, and (2) to evaluate the relationship between GPx-3 bioactivity and its protein concentration. Methods: Serum samples were collected from 50 normal healthy subjects and 70 septic patients. The reliable bioanalytical methods for GPx-3 protein concentration and bioactivity in human serum were developed and validated. Analyses of GPx-3 bioactivity and GPx-3 protein concentration were then performed. Results: Geometric mean GPx-3 bioactivity was 78.13. U/l for patients with sepsis, significantly lower than normal subjects with 108.21. U/l (p <. 0.0001). Similarly, the GPx-3 protein concentration was significantly lower in patients with sepsis than in normal subjects, with the mean GPx-3 value of 0.78 vs 3.10. μg/ml, respectively (p <. 0.0001). A positive correlation was observed between the GPx-3 bioactivity and its corresponding protein concentration in septic serum samples (R = 0.74, p <. 0.0001), regardless of gender or age difference. Conclusion: These findings suggest that the decrease in GPx-3 bioactivity observed in the septic patients was resulted from the significant sepsis-related decline of GPx-3 protein concentrations.

KW - Biomarker

KW - GPx-3 bioactivity

KW - GPx-3 protein concentration

KW - Selenium

KW - Sepsis

UR - http://www.scopus.com/inward/record.url?scp=85032985818&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85032985818&partnerID=8YFLogxK

U2 - 10.1016/j.cca.2017.10.031

DO - 10.1016/j.cca.2017.10.031

M3 - Article

AN - SCOPUS:85032985818

JO - Clinica Chimica Acta

JF - Clinica Chimica Acta

SN - 0009-8981

ER -