Abstract
Background: Serum glutathione peroxidase-3 (GPx-3) is known as a key selenoprotein with antioxidant properties. GPx-3 deficiency has been associated with sepsis. The objectives of this study are (1) to compare the GPx-3 protein concentrations and GPx-3 bioactivity in normal healthy subjects and septic patients, and (2) to evaluate the relationship between GPx-3 bioactivity and its protein concentration. Methods: Serum samples were collected from 50 normal healthy subjects and 70 septic patients. The reliable bioanalytical methods for GPx-3 protein concentration and bioactivity in human serum were developed and validated. Analyses of GPx-3 bioactivity and GPx-3 protein concentration were then performed. Results: Geometric mean GPx-3 bioactivity was 78.13. U/l for patients with sepsis, significantly lower than normal subjects with 108.21. U/l (p <. 0.0001). Similarly, the GPx-3 protein concentration was significantly lower in patients with sepsis than in normal subjects, with the mean GPx-3 value of 0.78 vs 3.10. μg/ml, respectively (p <. 0.0001). A positive correlation was observed between the GPx-3 bioactivity and its corresponding protein concentration in septic serum samples (R = 0.74, p <. 0.0001), regardless of gender or age difference. Conclusion: These findings suggest that the decrease in GPx-3 bioactivity observed in the septic patients was resulted from the significant sepsis-related decline of GPx-3 protein concentrations.
| Original language | English |
|---|---|
| Journal | Clinica Chimica Acta |
| DOIs | |
| Publication status | Published - 2019 |
| Externally published | Yes |
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Keywords
- Biomarker
- GPx-3 bioactivity
- GPx-3 protein concentration
- Selenium
- Sepsis
ASJC Scopus subject areas
- Biochemistry
- Clinical Biochemistry
- Biochemistry, medical
Cite this
Comparison of glutathione peroxidase-3 protein expression and enzyme bioactivity in normal subjects and patients with sepsis. / Lee, Wei Ju; Chen, Yen Ling; Chu, Yi Wen; Chien, Du Shieng.
In: Clinica Chimica Acta, 2019.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Comparison of glutathione peroxidase-3 protein expression and enzyme bioactivity in normal subjects and patients with sepsis
AU - Lee, Wei Ju
AU - Chen, Yen Ling
AU - Chu, Yi Wen
AU - Chien, Du Shieng
PY - 2019
Y1 - 2019
N2 - Background: Serum glutathione peroxidase-3 (GPx-3) is known as a key selenoprotein with antioxidant properties. GPx-3 deficiency has been associated with sepsis. The objectives of this study are (1) to compare the GPx-3 protein concentrations and GPx-3 bioactivity in normal healthy subjects and septic patients, and (2) to evaluate the relationship between GPx-3 bioactivity and its protein concentration. Methods: Serum samples were collected from 50 normal healthy subjects and 70 septic patients. The reliable bioanalytical methods for GPx-3 protein concentration and bioactivity in human serum were developed and validated. Analyses of GPx-3 bioactivity and GPx-3 protein concentration were then performed. Results: Geometric mean GPx-3 bioactivity was 78.13. U/l for patients with sepsis, significantly lower than normal subjects with 108.21. U/l (p <. 0.0001). Similarly, the GPx-3 protein concentration was significantly lower in patients with sepsis than in normal subjects, with the mean GPx-3 value of 0.78 vs 3.10. μg/ml, respectively (p <. 0.0001). A positive correlation was observed between the GPx-3 bioactivity and its corresponding protein concentration in septic serum samples (R = 0.74, p <. 0.0001), regardless of gender or age difference. Conclusion: These findings suggest that the decrease in GPx-3 bioactivity observed in the septic patients was resulted from the significant sepsis-related decline of GPx-3 protein concentrations.
AB - Background: Serum glutathione peroxidase-3 (GPx-3) is known as a key selenoprotein with antioxidant properties. GPx-3 deficiency has been associated with sepsis. The objectives of this study are (1) to compare the GPx-3 protein concentrations and GPx-3 bioactivity in normal healthy subjects and septic patients, and (2) to evaluate the relationship between GPx-3 bioactivity and its protein concentration. Methods: Serum samples were collected from 50 normal healthy subjects and 70 septic patients. The reliable bioanalytical methods for GPx-3 protein concentration and bioactivity in human serum were developed and validated. Analyses of GPx-3 bioactivity and GPx-3 protein concentration were then performed. Results: Geometric mean GPx-3 bioactivity was 78.13. U/l for patients with sepsis, significantly lower than normal subjects with 108.21. U/l (p <. 0.0001). Similarly, the GPx-3 protein concentration was significantly lower in patients with sepsis than in normal subjects, with the mean GPx-3 value of 0.78 vs 3.10. μg/ml, respectively (p <. 0.0001). A positive correlation was observed between the GPx-3 bioactivity and its corresponding protein concentration in septic serum samples (R = 0.74, p <. 0.0001), regardless of gender or age difference. Conclusion: These findings suggest that the decrease in GPx-3 bioactivity observed in the septic patients was resulted from the significant sepsis-related decline of GPx-3 protein concentrations.
KW - Biomarker
KW - GPx-3 bioactivity
KW - GPx-3 protein concentration
KW - Selenium
KW - Sepsis
UR - http://www.scopus.com/inward/record.url?scp=85032985818&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85032985818&partnerID=8YFLogxK
U2 - 10.1016/j.cca.2017.10.031
DO - 10.1016/j.cca.2017.10.031
M3 - Article
AN - SCOPUS:85032985818
JO - Clinica Chimica Acta
JF - Clinica Chimica Acta
SN - 0009-8981
ER -