Comparison of genome-wide DNA methylation in urothelial carcinomas of patients with and without arsenic exposure

Tse Yen Yang, Ling I. Hsu, Allen W. Chiu, Yeong Shiau Pu, Sheng Hsin Wang, Ya Tang Liao, Meei Maan Wu, Yuan Hung Wang, Chin Hao Chang, Te Chang Lee, Chien Jen Chen

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Abstract

Background: Arsenic is a well-documented carcinogen of human urothelial carcinoma (UC) with incompletely understood mechanisms. Objectives: This study aimed to compare the genome-wide DNA methylation profiles of arsenic-induced UC (AsUC) and non-arsenic-induced UC (Non-AsUC), and to assess associations between site-specific methylation levels and cumulative arsenic exposure. Methods: Genome-wide DNA methylation profiles in 14 AsUC and 14 non-AsUC were analyzed by Illumina Infinium methylation27 BeadChip and validated by bisulfite pyrosequencing. Mean methylation levels (β-) in AsUC and non-AsUC were compared by their ratio (β- ratio) and difference (δβ-). Associations between site-specific methylation levels in UC and cumulative arsenic exposure were examined. Results: Among 27,578 methylation sites analyzed, 231 sites had β- ratio >2 or 0.2 or

Original languageEnglish
Pages (from-to)57-63
Number of pages7
JournalEnvironmental Research
Volume128
DOIs
Publication statusPublished - Jan 1 2014

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Keywords

  • Arsenic
  • Cumulative Arsenic Exposure
  • DNA methylation
  • Urothelial carcinoma
  • β value

ASJC Scopus subject areas

  • Environmental Science(all)
  • Biochemistry

Cite this

Yang, T. Y., Hsu, L. I., Chiu, A. W., Pu, Y. S., Wang, S. H., Liao, Y. T., Wu, M. M., Wang, Y. H., Chang, C. H., Lee, T. C., & Chen, C. J. (2014). Comparison of genome-wide DNA methylation in urothelial carcinomas of patients with and without arsenic exposure. Environmental Research, 128, 57-63. https://doi.org/10.1016/j.envres.2013.10.006