Comparison of free radical formation induced by baicalein and pentamethyl-hydroxychromane in human promyelocytic leukemia cells using electron spin resonance

Yung Kai Huang, Ting Chen Chang, Joen Rong Sheu, Kuo Hsuan Wen, Duen Suey Chou

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2 Citations (Scopus)

Abstract

Baicalein and pentamethyl-hydroxychromane (PMC) have been investigated for use as antioxidants. However, antioxidants may stimulate free radical formation under certain conditions. The aim of our study was to determine whether PMC and baicalein exhibit both pro-oxidant and antioxidant activities in human promyelocytic leukemia (HL-60) cells. In this study, electron spin resonance spectrometry was used to investigate the effects of baicalein and PMC on free radical formation. In HL-60 cells, baicalein and PMC produced hydroxyl and phenoxyl radicals, respectively, but each inhibited radical formation by the other. The PMC pro-oxidant activity required H2O2, whereas baicalein produced hydroxyl radicals during the cell resting state only. The antioxidant effect of baicalein on PMC-induced oxidative stress in HL-60 cells may involve myeloperoxidase inhibition, which produces the myeloperoxidase- protein radical. Our investigation of the antioxidant effects of baicalein on arachidonic acid (AA)-induced oxidative stress in HL-60 cells showed that the baicalein-phenoxyl radical was the primary product, and that either carbon-centered or acyl radicals were the secondary products. However, the antioxidant effects of PMC on AA-induced oxidative stress produced only nonradical products. In conclusion, we showed that baicalein displayed both pro-oxidant and antioxidant activities in HL-60 cells. PMC exhibited no pro-oxidant activity during the cells' resting state but produced the PMC-phenoxyl radical in the presence of H2O2.The reaction of baicalein with AA in HL-60 cells produced baicalein-derived phenoxyl radicals that may initiate various pro-oxidative reactions. However, PMC does not produce radicals when it acts as an antioxidant. Thus, PMC is more beneficial as an antioxidant than baicalein.

Original languageEnglish
Pages (from-to)379-390
Number of pages12
JournalJournal of Food and Drug Analysis
Volume22
Issue number3
DOIs
Publication statusPublished - 2014

Fingerprint

electron paramagnetic resonance spectroscopy
Electron Spin Resonance Spectroscopy
leukemia
Free Radicals
Leukemia
Antioxidants
HL-60 Cells
antioxidants
oxidants
cells
arachidonic acid
Reactive Oxygen Species
myeloperoxidase
Arachidonic Acid
oxidative stress
Oxidative Stress
Hydroxyl Radical
antioxidant activity
Peroxidase
baicalein

Keywords

  • Antioxidant
  • Baicalein
  • Free radical
  • MPO
  • PMC
  • Pro-oxidant

ASJC Scopus subject areas

  • Food Science
  • Pharmacology

Cite this

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title = "Comparison of free radical formation induced by baicalein and pentamethyl-hydroxychromane in human promyelocytic leukemia cells using electron spin resonance",
abstract = "Baicalein and pentamethyl-hydroxychromane (PMC) have been investigated for use as antioxidants. However, antioxidants may stimulate free radical formation under certain conditions. The aim of our study was to determine whether PMC and baicalein exhibit both pro-oxidant and antioxidant activities in human promyelocytic leukemia (HL-60) cells. In this study, electron spin resonance spectrometry was used to investigate the effects of baicalein and PMC on free radical formation. In HL-60 cells, baicalein and PMC produced hydroxyl and phenoxyl radicals, respectively, but each inhibited radical formation by the other. The PMC pro-oxidant activity required H2O2, whereas baicalein produced hydroxyl radicals during the cell resting state only. The antioxidant effect of baicalein on PMC-induced oxidative stress in HL-60 cells may involve myeloperoxidase inhibition, which produces the myeloperoxidase- protein radical. Our investigation of the antioxidant effects of baicalein on arachidonic acid (AA)-induced oxidative stress in HL-60 cells showed that the baicalein-phenoxyl radical was the primary product, and that either carbon-centered or acyl radicals were the secondary products. However, the antioxidant effects of PMC on AA-induced oxidative stress produced only nonradical products. In conclusion, we showed that baicalein displayed both pro-oxidant and antioxidant activities in HL-60 cells. PMC exhibited no pro-oxidant activity during the cells' resting state but produced the PMC-phenoxyl radical in the presence of H2O2.The reaction of baicalein with AA in HL-60 cells produced baicalein-derived phenoxyl radicals that may initiate various pro-oxidative reactions. However, PMC does not produce radicals when it acts as an antioxidant. Thus, PMC is more beneficial as an antioxidant than baicalein.",
keywords = "Antioxidant, Baicalein, Free radical, MPO, PMC, Pro-oxidant",
author = "Huang, {Yung Kai} and Chang, {Ting Chen} and Sheu, {Joen Rong} and Wen, {Kuo Hsuan} and Chou, {Duen Suey}",
year = "2014",
doi = "10.1016/j.jfda.2014.01.018",
language = "English",
volume = "22",
pages = "379--390",
journal = "Journal of Food and Drug Analysis",
issn = "1021-9498",
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TY - JOUR

T1 - Comparison of free radical formation induced by baicalein and pentamethyl-hydroxychromane in human promyelocytic leukemia cells using electron spin resonance

AU - Huang, Yung Kai

AU - Chang, Ting Chen

AU - Sheu, Joen Rong

AU - Wen, Kuo Hsuan

AU - Chou, Duen Suey

PY - 2014

Y1 - 2014

N2 - Baicalein and pentamethyl-hydroxychromane (PMC) have been investigated for use as antioxidants. However, antioxidants may stimulate free radical formation under certain conditions. The aim of our study was to determine whether PMC and baicalein exhibit both pro-oxidant and antioxidant activities in human promyelocytic leukemia (HL-60) cells. In this study, electron spin resonance spectrometry was used to investigate the effects of baicalein and PMC on free radical formation. In HL-60 cells, baicalein and PMC produced hydroxyl and phenoxyl radicals, respectively, but each inhibited radical formation by the other. The PMC pro-oxidant activity required H2O2, whereas baicalein produced hydroxyl radicals during the cell resting state only. The antioxidant effect of baicalein on PMC-induced oxidative stress in HL-60 cells may involve myeloperoxidase inhibition, which produces the myeloperoxidase- protein radical. Our investigation of the antioxidant effects of baicalein on arachidonic acid (AA)-induced oxidative stress in HL-60 cells showed that the baicalein-phenoxyl radical was the primary product, and that either carbon-centered or acyl radicals were the secondary products. However, the antioxidant effects of PMC on AA-induced oxidative stress produced only nonradical products. In conclusion, we showed that baicalein displayed both pro-oxidant and antioxidant activities in HL-60 cells. PMC exhibited no pro-oxidant activity during the cells' resting state but produced the PMC-phenoxyl radical in the presence of H2O2.The reaction of baicalein with AA in HL-60 cells produced baicalein-derived phenoxyl radicals that may initiate various pro-oxidative reactions. However, PMC does not produce radicals when it acts as an antioxidant. Thus, PMC is more beneficial as an antioxidant than baicalein.

AB - Baicalein and pentamethyl-hydroxychromane (PMC) have been investigated for use as antioxidants. However, antioxidants may stimulate free radical formation under certain conditions. The aim of our study was to determine whether PMC and baicalein exhibit both pro-oxidant and antioxidant activities in human promyelocytic leukemia (HL-60) cells. In this study, electron spin resonance spectrometry was used to investigate the effects of baicalein and PMC on free radical formation. In HL-60 cells, baicalein and PMC produced hydroxyl and phenoxyl radicals, respectively, but each inhibited radical formation by the other. The PMC pro-oxidant activity required H2O2, whereas baicalein produced hydroxyl radicals during the cell resting state only. The antioxidant effect of baicalein on PMC-induced oxidative stress in HL-60 cells may involve myeloperoxidase inhibition, which produces the myeloperoxidase- protein radical. Our investigation of the antioxidant effects of baicalein on arachidonic acid (AA)-induced oxidative stress in HL-60 cells showed that the baicalein-phenoxyl radical was the primary product, and that either carbon-centered or acyl radicals were the secondary products. However, the antioxidant effects of PMC on AA-induced oxidative stress produced only nonradical products. In conclusion, we showed that baicalein displayed both pro-oxidant and antioxidant activities in HL-60 cells. PMC exhibited no pro-oxidant activity during the cells' resting state but produced the PMC-phenoxyl radical in the presence of H2O2.The reaction of baicalein with AA in HL-60 cells produced baicalein-derived phenoxyl radicals that may initiate various pro-oxidative reactions. However, PMC does not produce radicals when it acts as an antioxidant. Thus, PMC is more beneficial as an antioxidant than baicalein.

KW - Antioxidant

KW - Baicalein

KW - Free radical

KW - MPO

KW - PMC

KW - Pro-oxidant

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DO - 10.1016/j.jfda.2014.01.018

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VL - 22

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JO - Journal of Food and Drug Analysis

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SN - 1021-9498

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