Comparison of chemical compositions and osteoprotective effects of different sections of velvet antler

Sung Hui Tseng, Chun Hsien Sung, Lih Geeng Chen, Ying Jang Lai, Wei Shun Chang, Hsin Ching Sung, Ching Chiung Wang

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Ethnopharmacological relevance Velvet antlers (VA) have been claimed for centuries to have numerous medical benefits including strengthen bones. To investigate and compare the anti-osteoporotic activities from different sections of VA. Materials and methods Fresh VA prepared from farmed sika deers (Cervus nippon) was divided into upper (VAU), middle (VAM), and basal (VAB) sections. The chemical constituents and anti-osteoporotic effect of different sections from VA were evaluated using ovariectomized rats. Results Levels of water-soluble extracts, diluted alcoholic extract, amino acids, testosterone, insulin-like growth factor (IGF)-1and testosterone plus estradiol significantly differed among the different sections. Levels of these constituents were significantly higher in the upper section than in the basal section. Moreover, levels of testosterone and IGF-1 of the VAM were also significantly higher than those of the VAB. Calcium level increased downward from the tip with statistical significance. The strength of vertebrae increased in all VA-treated groups compared to the control, but only treatment with VAU and VAM increased the strength of the femur and the microarchitecure of the trabecular bone. Alkaline phosphatase levels of VAU-and VAM-treated groups significantly decreased, but osteocalcin did not significantly change. Moreover, VAU and VAM dose-dependently increased proliferation and mineralization of MC3T3-E1 cells. Conclusion Our study provides strong evidence for the regional differences in the effectiveness of velvet antler in treating osteoporosis. However, further studies are needed to elucidate the bioactive chemical constituents associated with the anti-osteoporotic effects of velvet antler.

Original languageEnglish
Pages (from-to)352-360
Number of pages9
JournalJournal of Ethnopharmacology
Volume151
Issue number1
DOIs
Publication statusPublished - Jan 10 2014

Fingerprint

Antlers
Testosterone
Somatomedins
Osteocalcin
Femur
Osteoporosis
Alkaline Phosphatase
Estradiol
Spine
Calcium
Amino Acids
Bone and Bones
Water

Keywords

  • Alkaline phosphatase
  • MC3T3-E1 cells
  • Micro-computerized tomography analysis
  • Osteocalcin
  • Ovariectomy
  • Velvet antler

ASJC Scopus subject areas

  • Pharmacology
  • Drug Discovery

Cite this

Comparison of chemical compositions and osteoprotective effects of different sections of velvet antler. / Tseng, Sung Hui; Sung, Chun Hsien; Chen, Lih Geeng; Lai, Ying Jang; Chang, Wei Shun; Sung, Hsin Ching; Wang, Ching Chiung.

In: Journal of Ethnopharmacology, Vol. 151, No. 1, 10.01.2014, p. 352-360.

Research output: Contribution to journalArticle

Tseng, Sung Hui ; Sung, Chun Hsien ; Chen, Lih Geeng ; Lai, Ying Jang ; Chang, Wei Shun ; Sung, Hsin Ching ; Wang, Ching Chiung. / Comparison of chemical compositions and osteoprotective effects of different sections of velvet antler. In: Journal of Ethnopharmacology. 2014 ; Vol. 151, No. 1. pp. 352-360.
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abstract = "Ethnopharmacological relevance Velvet antlers (VA) have been claimed for centuries to have numerous medical benefits including strengthen bones. To investigate and compare the anti-osteoporotic activities from different sections of VA. Materials and methods Fresh VA prepared from farmed sika deers (Cervus nippon) was divided into upper (VAU), middle (VAM), and basal (VAB) sections. The chemical constituents and anti-osteoporotic effect of different sections from VA were evaluated using ovariectomized rats. Results Levels of water-soluble extracts, diluted alcoholic extract, amino acids, testosterone, insulin-like growth factor (IGF)-1and testosterone plus estradiol significantly differed among the different sections. Levels of these constituents were significantly higher in the upper section than in the basal section. Moreover, levels of testosterone and IGF-1 of the VAM were also significantly higher than those of the VAB. Calcium level increased downward from the tip with statistical significance. The strength of vertebrae increased in all VA-treated groups compared to the control, but only treatment with VAU and VAM increased the strength of the femur and the microarchitecure of the trabecular bone. Alkaline phosphatase levels of VAU-and VAM-treated groups significantly decreased, but osteocalcin did not significantly change. Moreover, VAU and VAM dose-dependently increased proliferation and mineralization of MC3T3-E1 cells. Conclusion Our study provides strong evidence for the regional differences in the effectiveness of velvet antler in treating osteoporosis. However, further studies are needed to elucidate the bioactive chemical constituents associated with the anti-osteoporotic effects of velvet antler.",
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T1 - Comparison of chemical compositions and osteoprotective effects of different sections of velvet antler

AU - Tseng, Sung Hui

AU - Sung, Chun Hsien

AU - Chen, Lih Geeng

AU - Lai, Ying Jang

AU - Chang, Wei Shun

AU - Sung, Hsin Ching

AU - Wang, Ching Chiung

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N2 - Ethnopharmacological relevance Velvet antlers (VA) have been claimed for centuries to have numerous medical benefits including strengthen bones. To investigate and compare the anti-osteoporotic activities from different sections of VA. Materials and methods Fresh VA prepared from farmed sika deers (Cervus nippon) was divided into upper (VAU), middle (VAM), and basal (VAB) sections. The chemical constituents and anti-osteoporotic effect of different sections from VA were evaluated using ovariectomized rats. Results Levels of water-soluble extracts, diluted alcoholic extract, amino acids, testosterone, insulin-like growth factor (IGF)-1and testosterone plus estradiol significantly differed among the different sections. Levels of these constituents were significantly higher in the upper section than in the basal section. Moreover, levels of testosterone and IGF-1 of the VAM were also significantly higher than those of the VAB. Calcium level increased downward from the tip with statistical significance. The strength of vertebrae increased in all VA-treated groups compared to the control, but only treatment with VAU and VAM increased the strength of the femur and the microarchitecure of the trabecular bone. Alkaline phosphatase levels of VAU-and VAM-treated groups significantly decreased, but osteocalcin did not significantly change. Moreover, VAU and VAM dose-dependently increased proliferation and mineralization of MC3T3-E1 cells. Conclusion Our study provides strong evidence for the regional differences in the effectiveness of velvet antler in treating osteoporosis. However, further studies are needed to elucidate the bioactive chemical constituents associated with the anti-osteoporotic effects of velvet antler.

AB - Ethnopharmacological relevance Velvet antlers (VA) have been claimed for centuries to have numerous medical benefits including strengthen bones. To investigate and compare the anti-osteoporotic activities from different sections of VA. Materials and methods Fresh VA prepared from farmed sika deers (Cervus nippon) was divided into upper (VAU), middle (VAM), and basal (VAB) sections. The chemical constituents and anti-osteoporotic effect of different sections from VA were evaluated using ovariectomized rats. Results Levels of water-soluble extracts, diluted alcoholic extract, amino acids, testosterone, insulin-like growth factor (IGF)-1and testosterone plus estradiol significantly differed among the different sections. Levels of these constituents were significantly higher in the upper section than in the basal section. Moreover, levels of testosterone and IGF-1 of the VAM were also significantly higher than those of the VAB. Calcium level increased downward from the tip with statistical significance. The strength of vertebrae increased in all VA-treated groups compared to the control, but only treatment with VAU and VAM increased the strength of the femur and the microarchitecure of the trabecular bone. Alkaline phosphatase levels of VAU-and VAM-treated groups significantly decreased, but osteocalcin did not significantly change. Moreover, VAU and VAM dose-dependently increased proliferation and mineralization of MC3T3-E1 cells. Conclusion Our study provides strong evidence for the regional differences in the effectiveness of velvet antler in treating osteoporosis. However, further studies are needed to elucidate the bioactive chemical constituents associated with the anti-osteoporotic effects of velvet antler.

KW - Alkaline phosphatase

KW - MC3T3-E1 cells

KW - Micro-computerized tomography analysis

KW - Osteocalcin

KW - Ovariectomy

KW - Velvet antler

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