Comparison of cell response and surface characteristics on titanium implant with sla and slaffinity functionalization

Chiung Fang Huang, Hsi Jen Chiang, Hao Jan Lin, Hossein Hosseinkhani, Keng Liang Ou, Pei Wen Pengaz

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

To evaluate the cell responses of titanium oxide layer with the nanoporous micro-structure, an electrochemical anodization approach was proposed to create a nanoporous structure that superimposed onto the moderately rough sand-blasted, large-grit, acid-etched (SLA) Ti surface (denoted as SLAffinity-Ti). The osteoblast-like cells were cultured on SLAffinity-Ti and un-treated surfaces. Cell adhesion, viability, mRNA expressions as well as the productions of alkaline phosphatase (ALP) and osteocalcin (OC) were evaluated at predetermined intervals. The present results showed that cells had a high viability, attachment, and improved cell proliferation at day 7 and 14 when cultured on SLAffinity-Ti surface. The mRNA expressions of ALP and OC were upregulated while the type I collagen was not influenced. Additionally, the cells cultured on the SLAffinity-Ti surface produced a higher level of ALP and OC relative to cells on untreated surfaces. These findings confirmed that surface topography (nanoporous roughness) and TiO2 layers influenced the cell responses and the study revealed that cells grown on SLAffinity-Ti may improve osseointegration for Ti implants.

Original languageEnglish
JournalJournal of the Electrochemical Society
Volume161
Issue number3
DOIs
Publication statusPublished - 2014

Fingerprint

Titanium
osteocalcin
titanium
Osteocalcin
Phosphatases
phosphatases
Alkaline Phosphatase
cells
cultured cells
viability
Messenger RNA
Titanium oxides
grit
Osteoblasts
Cell adhesion
Cell proliferation
Surface topography
osteoblasts
Collagen Type I
Collagen

ASJC Scopus subject areas

  • Electrochemistry
  • Electronic, Optical and Magnetic Materials
  • Materials Chemistry
  • Surfaces, Coatings and Films
  • Renewable Energy, Sustainability and the Environment
  • Condensed Matter Physics

Cite this

Comparison of cell response and surface characteristics on titanium implant with sla and slaffinity functionalization. / Huang, Chiung Fang; Chiang, Hsi Jen; Lin, Hao Jan; Hosseinkhani, Hossein; Ou, Keng Liang; Pengaz, Pei Wen.

In: Journal of the Electrochemical Society, Vol. 161, No. 3, 2014.

Research output: Contribution to journalArticle

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AU - Ou, Keng Liang

AU - Pengaz, Pei Wen

PY - 2014

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