Comparison of arsenic methylation capacity and polymorphisms of arsenic methylation genes between bladder cancer and upper tract urothelial carcinoma

Chao Yuan Huang, Ying Chin Lin, Horng Sheng Shiue, Wei Jen Chen, Chien Tien Su, Yeong Shiau Pu, Pui Lam Ao, Yu Mei Hsueh

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Arsenic exposure is an environmental risk factor for urothelial carcinoma (UC). The natural history of upper tract urothelial carcinoma (UTUC) differs from that of bladder cancer (BC). However, the risk factors of BC and UTUC are not exactly the same and should be discussed separately. The aims of this study were to evaluate 1) the association between arsenic methylation capacity and UTUC and/or BC, separately, and 2) the association between polymorphisms of the arsenic metabolism-related genes AS3MT, GSTOs, and PNP against BC and/or UTUC, separately. We conducted a hospital-based study and collected 216 BC and 212 UTUC cases, and 813 healthy controls, from September 2007 to October 2011. Urinary arsenic profiles were measured using high-performance liquid chromatography-hydride generator-atomic absorption spectrometry. The polymorphisms of AS3MT, GSTO, and PNP were identified using the Sequenom MassARRAY platform with iPLEX Gold chemistry. We found that inefficient arsenic methylation capacity was associated with BC in a significant dose-response relationship, but only found that high urinary total arsenic concentration was related to the risk of UTUC, also in a significant dose-response manner. Those with a total urinary arsenic level of > 30.28 μg/L compared to ≤ 9.78 μg/L, had a odds ratio (OR), and 95% confidence interval (CI) of UTUC, of 4.80 (2.22–10.39). The polymorphisms of AS3MT rs11191438, AS3MT rs10748835, and AS3MT rs1046778 were related to the risk of BC and UTUC, while the polymorphisms of AS3MT rs3740393, AS3MT rs11191453, and AS3MT rs11191454 were associated with arsenic methylation capacity. The AS3MT gene polymorphisms and arsenic methylation capacity appear to independently affect the risk of BC and UTUC.

Original languageEnglish
Pages (from-to)64-73
Number of pages10
JournalToxicology Letters
Volume295
DOIs
Publication statusPublished - Oct 1 2018

Fingerprint

Methylation
Arsenic
Polymorphism
Urinary Bladder Neoplasms
Genes
Carcinoma
Association reactions
Atomic absorption spectrometry
High performance liquid chromatography
Environmental Exposure
Metabolism
Hydrides
Gold
Natural History
Spectrum Analysis
Odds Ratio
High Pressure Liquid Chromatography
Confidence Intervals

Keywords

  • Arsenic
  • Arsenic methylationAS3MTPNPGSTO
  • Bladder cancer
  • Polymorphism
  • Upper tract urothelial carcinoma

ASJC Scopus subject areas

  • Toxicology

Cite this

Comparison of arsenic methylation capacity and polymorphisms of arsenic methylation genes between bladder cancer and upper tract urothelial carcinoma. / Huang, Chao Yuan; Lin, Ying Chin; Shiue, Horng Sheng; Chen, Wei Jen; Su, Chien Tien; Pu, Yeong Shiau; Ao, Pui Lam; Hsueh, Yu Mei.

In: Toxicology Letters, Vol. 295, 01.10.2018, p. 64-73.

Research output: Contribution to journalArticle

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AU - Su, Chien Tien

AU - Pu, Yeong Shiau

AU - Ao, Pui Lam

AU - Hsueh, Yu Mei

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