Comparison of a nonradiometric liquid-medium method (MB REDOX) with the BACTEC system for growth and identification of mycobacteria in clinical specimens

Yung Ching Liu, Tsi S. Huang, Wen Kuei Huang

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Early identification of tuberculosis in the clinical setting is of great importance in order for specific therapy to be swiftly initiated. MB REDOX (Heipha Diagnostika), a growth-based medium without radioactive materials, was evaluated and was compared to the BACTEC system for detection of mycobacteria, including the Mycobacterium tuberculosis complex and atypical mycobacteria. MB REDOX consists of a Kirchner medium enriched with growth- promoting additives, antibiotic compounds, and a redox indicator which can be monitored to detect growth of mycobacteria with the naked eye. MB REDOX only detects growth and cannot differentiate the M. tuberculosis complex (M. tuberculosis, M. bovis, and M. africanum) from other species of Mycobacterium. Therefore, PCR-restriction fragment length polymorphism analysis (PRA) was used in this investigation to identify to the species level organisms showing positive growth with MB REDOX. Our data demonstrate the usefulness of MB REDOX for the detection of mycobacteria in clinical specimens. The rate of detection of M. tuberculosis complex with MB REDOX (84.3%) was higher than that with the BACTEC system (68.6%). When combined with PRA for species identification, MB REDOX is easy to perform and is suited to most clinical laboratory settings for the detection and identification of mycobacteria.

Original languageEnglish
Pages (from-to)4048-4050
Number of pages3
JournalJournal of Clinical Microbiology
Volume37
Issue number12
Publication statusPublished - Dec 1999
Externally publishedYes

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Mycobacterium
Mycobacterium tuberculosis
Growth
Nontuberculous Mycobacteria
Restriction Fragment Length Polymorphisms
Oxidation-Reduction
Tuberculosis
Anti-Bacterial Agents
Polymerase Chain Reaction
Therapeutics

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Cite this

Comparison of a nonradiometric liquid-medium method (MB REDOX) with the BACTEC system for growth and identification of mycobacteria in clinical specimens. / Liu, Yung Ching; Huang, Tsi S.; Huang, Wen Kuei.

In: Journal of Clinical Microbiology, Vol. 37, No. 12, 12.1999, p. 4048-4050.

Research output: Contribution to journalArticle

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