Comparison between the Hybrid Capture II test and an SPF1/GP6+ PCR-based assay for detection of human papillomavirus DNA in cervical swab samples

Shang Lang Huang, Angel Chao, Swei Hsueh, Fang Yu Chao, Chu Chun Huang, Jung Erh Yang, Ching Yu Lin, Chiu Cho Yan, Hung Hsueh Chou, Kuan Gen Huang, Huei Jean Huang, Tzu I. Wu, Mao Jung Tseng, Jian Tai Qiu, Cheng Tao Lin, Ting Chang Chang, Chyong Huey Lai

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Abstract

We compared the efficacy of human papillomavirus (HPV) DNA detection between a PCR-based genechip (Easychip HPV Blot [hereafter referred to as HPV Blot]; King Car, Taiwan) method and Hybrid Capture II (HCII; Digene, Gaithersburg, MD) in women with previous normal (n = 146) or abnormal (≥atypical squamous cells of undetermined significance [ASCUS] [n = 208]) cytology. A total of 354 cervical swab samples were collected for HPV DNA assay by both HCII and SPF1/GP6+ PCR followed by HPV Blot tests. Colposcopy-directed biopsy was performed if clinically indicated. Of the 354 samples, HPV-positive rates by these two methods (HCII and HPV Blot) were 12.6% and 18.2% in 143 normal samples, 36.2% and 45.7% in 105 ASCUS samples, 57.4% and 57.4% in 94 low-grade squamous intraepithelial lesion samples, and 83.3% and 75.0% in 12 high-grade squamous intraepithelial lesion samples, respectively. The concordance of HPV Blot and HCII was 80.8% (286/354), and the agreement between the methods (κ value, 0.68) was substantial. Discrepancies were further investigated by at least one of the following three methods: direct sequencing, type-specific PCR, and HPV Blot genotyping of cervical biopsy tissue. In the 15 HCII-positive samples, HPV Blot detected only non-HCII HPV genotypes; results of further verification methods were consistent with the latter test in the 15 samples. Of the 20 samples with HCII-negative and HPV Blot-positive results, 18 were found to contain the 13 HCII high-risk genotypes by verification methods. In only 16.7% (3/18) of the HCII-positive but HPV Blot-negative samples, further studies detected the 13 HCII genotypes. We conclude that HPV Blot seemed comparable to HCII for detection of HPV DNA in cervical swab samples.

Original languageEnglish
Pages (from-to)1733-1739
Number of pages7
JournalJournal of Clinical Microbiology
Volume44
Issue number5
DOIs
Publication statusPublished - May 2006
Externally publishedYes

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Polymerase Chain Reaction
DNA
Genotype
Biopsy
Colposcopy
Taiwan
Cell Biology

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

Cite this

Comparison between the Hybrid Capture II test and an SPF1/GP6+ PCR-based assay for detection of human papillomavirus DNA in cervical swab samples. / Huang, Shang Lang; Chao, Angel; Hsueh, Swei; Chao, Fang Yu; Huang, Chu Chun; Yang, Jung Erh; Lin, Ching Yu; Yan, Chiu Cho; Chou, Hung Hsueh; Huang, Kuan Gen; Huang, Huei Jean; Wu, Tzu I.; Tseng, Mao Jung; Qiu, Jian Tai; Lin, Cheng Tao; Chang, Ting Chang; Lai, Chyong Huey.

In: Journal of Clinical Microbiology, Vol. 44, No. 5, 05.2006, p. 1733-1739.

Research output: Contribution to journalArticle

Huang, SL, Chao, A, Hsueh, S, Chao, FY, Huang, CC, Yang, JE, Lin, CY, Yan, CC, Chou, HH, Huang, KG, Huang, HJ, Wu, TI, Tseng, MJ, Qiu, JT, Lin, CT, Chang, TC & Lai, CH 2006, 'Comparison between the Hybrid Capture II test and an SPF1/GP6+ PCR-based assay for detection of human papillomavirus DNA in cervical swab samples', Journal of Clinical Microbiology, vol. 44, no. 5, pp. 1733-1739. https://doi.org/10.1128/JCM.44.5.1733-1739.2006
Huang, Shang Lang ; Chao, Angel ; Hsueh, Swei ; Chao, Fang Yu ; Huang, Chu Chun ; Yang, Jung Erh ; Lin, Ching Yu ; Yan, Chiu Cho ; Chou, Hung Hsueh ; Huang, Kuan Gen ; Huang, Huei Jean ; Wu, Tzu I. ; Tseng, Mao Jung ; Qiu, Jian Tai ; Lin, Cheng Tao ; Chang, Ting Chang ; Lai, Chyong Huey. / Comparison between the Hybrid Capture II test and an SPF1/GP6+ PCR-based assay for detection of human papillomavirus DNA in cervical swab samples. In: Journal of Clinical Microbiology. 2006 ; Vol. 44, No. 5. pp. 1733-1739.
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abstract = "We compared the efficacy of human papillomavirus (HPV) DNA detection between a PCR-based genechip (Easychip HPV Blot [hereafter referred to as HPV Blot]; King Car, Taiwan) method and Hybrid Capture II (HCII; Digene, Gaithersburg, MD) in women with previous normal (n = 146) or abnormal (≥atypical squamous cells of undetermined significance [ASCUS] [n = 208]) cytology. A total of 354 cervical swab samples were collected for HPV DNA assay by both HCII and SPF1/GP6+ PCR followed by HPV Blot tests. Colposcopy-directed biopsy was performed if clinically indicated. Of the 354 samples, HPV-positive rates by these two methods (HCII and HPV Blot) were 12.6{\%} and 18.2{\%} in 143 normal samples, 36.2{\%} and 45.7{\%} in 105 ASCUS samples, 57.4{\%} and 57.4{\%} in 94 low-grade squamous intraepithelial lesion samples, and 83.3{\%} and 75.0{\%} in 12 high-grade squamous intraepithelial lesion samples, respectively. The concordance of HPV Blot and HCII was 80.8{\%} (286/354), and the agreement between the methods (κ value, 0.68) was substantial. Discrepancies were further investigated by at least one of the following three methods: direct sequencing, type-specific PCR, and HPV Blot genotyping of cervical biopsy tissue. In the 15 HCII-positive samples, HPV Blot detected only non-HCII HPV genotypes; results of further verification methods were consistent with the latter test in the 15 samples. Of the 20 samples with HCII-negative and HPV Blot-positive results, 18 were found to contain the 13 HCII high-risk genotypes by verification methods. In only 16.7{\%} (3/18) of the HCII-positive but HPV Blot-negative samples, further studies detected the 13 HCII genotypes. We conclude that HPV Blot seemed comparable to HCII for detection of HPV DNA in cervical swab samples.",
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AU - Huang, Shang Lang

AU - Chao, Angel

AU - Hsueh, Swei

AU - Chao, Fang Yu

AU - Huang, Chu Chun

AU - Yang, Jung Erh

AU - Lin, Ching Yu

AU - Yan, Chiu Cho

AU - Chou, Hung Hsueh

AU - Huang, Kuan Gen

AU - Huang, Huei Jean

AU - Wu, Tzu I.

AU - Tseng, Mao Jung

AU - Qiu, Jian Tai

AU - Lin, Cheng Tao

AU - Chang, Ting Chang

AU - Lai, Chyong Huey

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N2 - We compared the efficacy of human papillomavirus (HPV) DNA detection between a PCR-based genechip (Easychip HPV Blot [hereafter referred to as HPV Blot]; King Car, Taiwan) method and Hybrid Capture II (HCII; Digene, Gaithersburg, MD) in women with previous normal (n = 146) or abnormal (≥atypical squamous cells of undetermined significance [ASCUS] [n = 208]) cytology. A total of 354 cervical swab samples were collected for HPV DNA assay by both HCII and SPF1/GP6+ PCR followed by HPV Blot tests. Colposcopy-directed biopsy was performed if clinically indicated. Of the 354 samples, HPV-positive rates by these two methods (HCII and HPV Blot) were 12.6% and 18.2% in 143 normal samples, 36.2% and 45.7% in 105 ASCUS samples, 57.4% and 57.4% in 94 low-grade squamous intraepithelial lesion samples, and 83.3% and 75.0% in 12 high-grade squamous intraepithelial lesion samples, respectively. The concordance of HPV Blot and HCII was 80.8% (286/354), and the agreement between the methods (κ value, 0.68) was substantial. Discrepancies were further investigated by at least one of the following three methods: direct sequencing, type-specific PCR, and HPV Blot genotyping of cervical biopsy tissue. In the 15 HCII-positive samples, HPV Blot detected only non-HCII HPV genotypes; results of further verification methods were consistent with the latter test in the 15 samples. Of the 20 samples with HCII-negative and HPV Blot-positive results, 18 were found to contain the 13 HCII high-risk genotypes by verification methods. In only 16.7% (3/18) of the HCII-positive but HPV Blot-negative samples, further studies detected the 13 HCII genotypes. We conclude that HPV Blot seemed comparable to HCII for detection of HPV DNA in cervical swab samples.

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