Comparing Molecular Methods for Early Detection and Serotyping of Enteroviruses in Throat Swabs of Pediatric Patients

Pai Shan Chiang, Mei Liang Huang, Shu Ting Luo, Tzou Yien Lin, Kuo Chien Tsao, Min Shi Lee

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Background: Enteroviruses include over 100 serotypes and usually cause self-limited infections with non-specific symptoms in children, with the exceptions of polioviruses and enterovirus 71 which frequently cause neurologic complications. Therefore, early detection and serotyping of enteroviruses are critical in clinical management and disease surveillance. Traditional methods for detection and serotyping of enteroviruses are virus isolation and immunofluorescence assay, which are time-consuming. In this study, we compare virus isolation and two molecular tests for detection and serotyping of enteroviruses in clinical samples. Methods: One hundred and ten throat swabs were collected from pediatric outpatients with enterovirus-like illnesses (hand-foot-mouth disease, herpangina, and non-specific febrile illness). Virus isolation was conducted using multiple cell lines and isolated viruses were serotyped using immunofluorescent assay. In the molecular tests, a semi-nested RT-PCR and a novel CODEHOP platform were used to detect the 5′UTR and VP1 genes of enteroviruses, respectively. Amplified nucleotides were sequenced and genotyped. Results: Among the 110 cases, 39(35%), 52(47%), and 46(42%) were tested positive with these three tests, respectively. Using the consensus results of these three tests as the gold standard, agreement of the VP1 CODEHOP test was 96%, which is higher than those of the virus isolation (89%) and the 5′-UTR test (88%). The VP1 CODEHOP test also has the best performance on serotyping confirmed with serum neutralization tests. Conclusions: The VP1 CODEHOP test performed well for detection and serotyping of enteroviruses in clinical specimens and could reduce unnecessary hospitalization cares during enterovirus seasons.

Original languageEnglish
Article numbere48269
JournalPLoS One
Volume7
Issue number10
DOIs
Publication statusPublished - Oct 25 2012
Externally publishedYes

Fingerprint

Serotyping
Enterovirus
Pediatrics
throat
Pharynx
Viruses
viruses
Assays
testing
methodology
5' Untranslated Regions
fluorescent antibody technique
Herpangina
hand, foot and mouth disease
Hand, Foot and Mouth Disease
Nucleotides
Genes
Cells
Enterovirus C
Neutralization Tests

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Comparing Molecular Methods for Early Detection and Serotyping of Enteroviruses in Throat Swabs of Pediatric Patients. / Chiang, Pai Shan; Huang, Mei Liang; Luo, Shu Ting; Lin, Tzou Yien; Tsao, Kuo Chien; Lee, Min Shi.

In: PLoS One, Vol. 7, No. 10, e48269, 25.10.2012.

Research output: Contribution to journalArticle

Chiang, Pai Shan ; Huang, Mei Liang ; Luo, Shu Ting ; Lin, Tzou Yien ; Tsao, Kuo Chien ; Lee, Min Shi. / Comparing Molecular Methods for Early Detection and Serotyping of Enteroviruses in Throat Swabs of Pediatric Patients. In: PLoS One. 2012 ; Vol. 7, No. 10.
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