Comparing cytotoxicity of ZnO nanoparticles in exposure to serum/serum-free cell culture medium

I. L. Hsiao, Y. J. Huang

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The aim of this study is to evaluate the influence of serum [fetal bovine serum (FBS)] in cell culture medium on the nano and micro-sized ZnO toxicity to human lung epithelial cells (A549). Particles agglomeration and sedimentation state in medium were respectively measured by Zetasizer and UV-vis spectrometer. Methyl thiazolyl tetrazolium (MTT) and interleukin 8 (IL-8) analysis were selected for cytotoxicity assays. We found ZnO particles with a smaller primary size increased to a relative smaller secondary size in both serum/serum-free Dulbecco's modified Eagle's medium (DMEM), and caused the size-dependent cytotoxicity effect on ZnO nanoparticles. However, negative charges of serum caused the zeta potential of ZnO slightly decreased to -8 ~ -10 mV in DMEM, which resulted in smaller hydrodynamic size and much slower sedimentation than in serum free medium, leading to similarly lower toxicity.

Original languageEnglish
Title of host publicationNanotechnology 2010
Subtitle of host publicationBio Sensors, Instruments, Medical, Environment and Energy - Technical Proceedings of the 2010 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2010
Pages557-560
Number of pages4
Volume3
Publication statusPublished - 2010
Externally publishedYes
EventNanotechnology 2010: Bio Sensors, Instruments, Medical, Environment and Energy - 2010 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2010 - Anaheim, CA, United States
Duration: Jun 21 2010Jun 24 2010

Conference

ConferenceNanotechnology 2010: Bio Sensors, Instruments, Medical, Environment and Energy - 2010 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2010
CountryUnited States
CityAnaheim, CA
Period6/21/106/24/10

Keywords

  • A549
  • Cytotoxicity
  • Nanoparticles
  • Zinc oxide

ASJC Scopus subject areas

  • Bioengineering
  • Biomedical Engineering
  • Electrical and Electronic Engineering

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  • Cite this

    Hsiao, I. L., & Huang, Y. J. (2010). Comparing cytotoxicity of ZnO nanoparticles in exposure to serum/serum-free cell culture medium. In Nanotechnology 2010: Bio Sensors, Instruments, Medical, Environment and Energy - Technical Proceedings of the 2010 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2010 (Vol. 3, pp. 557-560)