Comparative tyrosine-kinase profiles in colorectal cancers

Enhanced arg expression in carcinoma as compared with adenoma and normal mucosa

Wei Shone Chen, Hsing Jien Kung, Wen Kuang Yang, Wen Chang Lin

Research output: Contribution to journalArticle

65 Citations (Scopus)

Abstract

There is strong evidence that tyrosine kinases are involved in the regulation of cellular growth and tumor progression. Over-expressions of tyrosine kinases have been documented in a number of neoplasms. To study the roles of tyrosine kinases in colon cancer, we developed a tyrosine-kinase- expression profile for each of the four different stages of colon carcinogenesis, using normal colon mucosa, adenomatous polyps, primary carcinoma and hepatic metastases collected from the same patient. We identified 30 tyrosine kinases expressed in these tissues: they include 10 non-receptor tyrosine kinases (yes, fyn, lyn, brk, abl, arg, jak1, jak3, tyk2 and itk), 17 receptor tyrosine kinases (erbB2, PDGF-Rα, PDGF-Rβ, kit, c- fins, met, ron, FGF-R1, FGF-R2, FGF-R3, FGF-R4, cek5, tie-1, tkt, axl, sky and Ins-R), 2 dual kinases (mek and sek) and one possible novel kinase. Among these kinases, arg kinase appears to be expressed at a higher level in primary carcinoma and metastatic tumor than in adjacent normal mucosa or adenomatous polyp. This result was confirmed by extensive analysis of 50 additional matched sets of normal colon and colon-tumor specimens, using arg- specific primers and RT-PCR reactions. This study identifies a possible role for arg tyrosine kinase in colon carcinogenesis, especially in the transition from adenoma to carcinoma.

Original languageEnglish
Pages (from-to)579-584
Number of pages6
JournalInternational Journal of Cancer
Volume83
Issue number5
DOIs
Publication statusPublished - Nov 10 1999
Externally publishedYes

Fingerprint

Adenoma
Protein-Tyrosine Kinases
Colorectal Neoplasms
Mucous Membrane
Colon
Carcinoma
Phosphotransferases
Adenomatous Polyps
Neoplasms
Carcinogenesis
Proto-Oncogene Proteins c-fyn
Colonic Neoplasms
Neoplasm Metastasis
Polymerase Chain Reaction
Liver
Growth

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Comparative tyrosine-kinase profiles in colorectal cancers : Enhanced arg expression in carcinoma as compared with adenoma and normal mucosa. / Chen, Wei Shone; Kung, Hsing Jien; Yang, Wen Kuang; Lin, Wen Chang.

In: International Journal of Cancer, Vol. 83, No. 5, 10.11.1999, p. 579-584.

Research output: Contribution to journalArticle

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