Comparative Study of Alcohol Metabolism in Stroke-Prone Spontaneously Hypertensive Rats and Wistar-Kyoto Rats Fed Normal or Low Levels of Dietary Protein

Suh Ching Yang, Michiko Ito, Yuji Furukawa, Shuichi Kimura

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6 Citations (Scopus)

Abstract

Our previous experiments have shown that the appetite or preference for alcohol is affected by the rat strain and nutritional status, such as dietary protein levels. To determine the affected factors in alcohol preference, the alcohol metabolism in SHRSP (stroke-prone spontaneously hypertensive rats) and WKY (Wistar-Kyoto) rats fed with the standard level (15%) or low level (5%) purified egg protein diet (PEP) was investigated. The animals were kept on the experimental diets for 4 weeks. After 12h fasting, a 15% ethanol solution was given in a dose of 100mg ethanol per 100g body weight with a gastric probe to all animals and the blood ethanol and acetaldehyde levels were determined. Compared with 15% PEP diet-fed SHRSP, WKY showed higher levels of blood ethanol and acetaldehyde. Furthermore, the same results were also observed in SHRSP and WKY fed with 5% PEP diet. On the other hand, regardless of the rat strain, rats fed a low level protein diet showed higher blood ethanol and acetaldehyde levels. We also found that there was no significant change in alcohol dehydrogenase (ADH) activity and acetaldehyde dehydrogenase (ALDH) activity between SHRSP and WKY. However, both SHRSP and WKY fed a 15% PEP diet showed higher ADH and ALDH activity compared with rats fed the 5% PEP diet. These results suggested that the affected factors of preference for alcohol may be correlated with blood ethanol and acetaldehyde levels after alcohol intake.

Original languageEnglish
Pages (from-to)547-555
Number of pages9
JournalJournal of Nutritional Science and Vitaminology
Volume40
Issue number6
DOIs
Publication statusPublished - 1994
Externally publishedYes

Fingerprint

Inbred WKY Rats
Dietary Proteins
Inbred SHR Rats
Stroke
Alcohols
Diet
Egg Proteins
Ethanol
Acetaldehyde
Alcohol Dehydrogenase
Protein-Restricted Diet
Appetite
Nutritional Status
Fasting
Stomach
Body Weight

Keywords

  • acetaldehyde dehydrogenase (ALDH)
  • alcohol dehydrogenase (ADH)
  • appetite or preference for alcohol
  • blood ethanol and acetaldehyde levels
  • dietary protein levels
  • stroke-prone spontaneously hypertensive rats (SHRSP)
  • Wistar-Kyoto rats (WKY)

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

Cite this

@article{b1b7e4a3673d4cee8c9458d089fdaef8,
title = "Comparative Study of Alcohol Metabolism in Stroke-Prone Spontaneously Hypertensive Rats and Wistar-Kyoto Rats Fed Normal or Low Levels of Dietary Protein",
abstract = "Our previous experiments have shown that the appetite or preference for alcohol is affected by the rat strain and nutritional status, such as dietary protein levels. To determine the affected factors in alcohol preference, the alcohol metabolism in SHRSP (stroke-prone spontaneously hypertensive rats) and WKY (Wistar-Kyoto) rats fed with the standard level (15{\%}) or low level (5{\%}) purified egg protein diet (PEP) was investigated. The animals were kept on the experimental diets for 4 weeks. After 12h fasting, a 15{\%} ethanol solution was given in a dose of 100mg ethanol per 100g body weight with a gastric probe to all animals and the blood ethanol and acetaldehyde levels were determined. Compared with 15{\%} PEP diet-fed SHRSP, WKY showed higher levels of blood ethanol and acetaldehyde. Furthermore, the same results were also observed in SHRSP and WKY fed with 5{\%} PEP diet. On the other hand, regardless of the rat strain, rats fed a low level protein diet showed higher blood ethanol and acetaldehyde levels. We also found that there was no significant change in alcohol dehydrogenase (ADH) activity and acetaldehyde dehydrogenase (ALDH) activity between SHRSP and WKY. However, both SHRSP and WKY fed a 15{\%} PEP diet showed higher ADH and ALDH activity compared with rats fed the 5{\%} PEP diet. These results suggested that the affected factors of preference for alcohol may be correlated with blood ethanol and acetaldehyde levels after alcohol intake.",
keywords = "acetaldehyde dehydrogenase (ALDH), alcohol dehydrogenase (ADH), appetite or preference for alcohol, blood ethanol and acetaldehyde levels, dietary protein levels, stroke-prone spontaneously hypertensive rats (SHRSP), Wistar-Kyoto rats (WKY)",
author = "Yang, {Suh Ching} and Michiko Ito and Yuji Furukawa and Shuichi Kimura",
year = "1994",
doi = "10.3177/jnsv.40.547",
language = "English",
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journal = "Journal of Nutritional Science and Vitaminology",
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T1 - Comparative Study of Alcohol Metabolism in Stroke-Prone Spontaneously Hypertensive Rats and Wistar-Kyoto Rats Fed Normal or Low Levels of Dietary Protein

AU - Yang, Suh Ching

AU - Ito, Michiko

AU - Furukawa, Yuji

AU - Kimura, Shuichi

PY - 1994

Y1 - 1994

N2 - Our previous experiments have shown that the appetite or preference for alcohol is affected by the rat strain and nutritional status, such as dietary protein levels. To determine the affected factors in alcohol preference, the alcohol metabolism in SHRSP (stroke-prone spontaneously hypertensive rats) and WKY (Wistar-Kyoto) rats fed with the standard level (15%) or low level (5%) purified egg protein diet (PEP) was investigated. The animals were kept on the experimental diets for 4 weeks. After 12h fasting, a 15% ethanol solution was given in a dose of 100mg ethanol per 100g body weight with a gastric probe to all animals and the blood ethanol and acetaldehyde levels were determined. Compared with 15% PEP diet-fed SHRSP, WKY showed higher levels of blood ethanol and acetaldehyde. Furthermore, the same results were also observed in SHRSP and WKY fed with 5% PEP diet. On the other hand, regardless of the rat strain, rats fed a low level protein diet showed higher blood ethanol and acetaldehyde levels. We also found that there was no significant change in alcohol dehydrogenase (ADH) activity and acetaldehyde dehydrogenase (ALDH) activity between SHRSP and WKY. However, both SHRSP and WKY fed a 15% PEP diet showed higher ADH and ALDH activity compared with rats fed the 5% PEP diet. These results suggested that the affected factors of preference for alcohol may be correlated with blood ethanol and acetaldehyde levels after alcohol intake.

AB - Our previous experiments have shown that the appetite or preference for alcohol is affected by the rat strain and nutritional status, such as dietary protein levels. To determine the affected factors in alcohol preference, the alcohol metabolism in SHRSP (stroke-prone spontaneously hypertensive rats) and WKY (Wistar-Kyoto) rats fed with the standard level (15%) or low level (5%) purified egg protein diet (PEP) was investigated. The animals were kept on the experimental diets for 4 weeks. After 12h fasting, a 15% ethanol solution was given in a dose of 100mg ethanol per 100g body weight with a gastric probe to all animals and the blood ethanol and acetaldehyde levels were determined. Compared with 15% PEP diet-fed SHRSP, WKY showed higher levels of blood ethanol and acetaldehyde. Furthermore, the same results were also observed in SHRSP and WKY fed with 5% PEP diet. On the other hand, regardless of the rat strain, rats fed a low level protein diet showed higher blood ethanol and acetaldehyde levels. We also found that there was no significant change in alcohol dehydrogenase (ADH) activity and acetaldehyde dehydrogenase (ALDH) activity between SHRSP and WKY. However, both SHRSP and WKY fed a 15% PEP diet showed higher ADH and ALDH activity compared with rats fed the 5% PEP diet. These results suggested that the affected factors of preference for alcohol may be correlated with blood ethanol and acetaldehyde levels after alcohol intake.

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