Comparative molecular analysis of community-associated and healthcare-associated methicillin-resistant Staphylococcus aureus isolates from children in northern Taiwan

Y. C. Huang, C. F. Ho, C. J. Chen, L. H. Su, Tzou-Yien Lin

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

From August 2004 to July 2005, 210 clinical methicillin-resistant Staphylococcus aureus (MRSA) isolates were collected prospectively from 173 children admitted to Chang Gung Children's Hospital in Taiwan. A comparative molecular analysis of the 111 community-associated (CA) isolates from 102 children and the 99 healthcare-associated (HA) isolates from 71 children was conducted. In comparison to the HA isolates (31%), the CA isolates (90%) were more likely to have been isolated from pus (p <5 × 10-8). For each patient with MRSA infection, only the first isolate was selected for molecular analysis. The molecular characteristics differed significantly between the CA and the HA isolates (p < 5 × 10-8). The clone characterized as sequence type (ST)59/pulsotype D (similar to USA1000)/staphylococcal chromosomal cassette (SCC) mec VT/ Panton-Valentine leukocidin (PVL)-positive accounted for 69% of the CA isolates, and another clone, characterized as ST239/pulsotype. A (Hungary clone)/SCCmec III/PVL-negative, accounted for 45% of the 71 HA isolates. The CA clone of ST59 also accounted for 20% of the HA isolates, including 47% of the 17 community-onset isolates. It was concluded that the molecular characteristics of clinical MRSA isolates from children differed significantly between the CA and the HA isolates in northern Taiwan. However, the CA clone of ST59 was also identified in a substantial proportion of HA isolates.

Original languageEnglish
Pages (from-to)1167-1172
Number of pages6
JournalClinical Microbiology and Infection
Volume14
Issue number12
DOIs
Publication statusPublished - Jan 1 2008
Externally publishedYes

Fingerprint

Community Health Services
Methicillin-Resistant Staphylococcus aureus
Taiwan
Clone Cells
Delivery of Health Care
Suppuration
Hungary
Infection

Keywords

  • Community-associated
  • Healthcare-associated
  • Methicillin-resistant Staphylococcus aureus
  • Molecular characteristics
  • Taiwan

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

Cite this

Comparative molecular analysis of community-associated and healthcare-associated methicillin-resistant Staphylococcus aureus isolates from children in northern Taiwan. / Huang, Y. C.; Ho, C. F.; Chen, C. J.; Su, L. H.; Lin, Tzou-Yien.

In: Clinical Microbiology and Infection, Vol. 14, No. 12, 01.01.2008, p. 1167-1172.

Research output: Contribution to journalArticle

@article{0c459b4496bc49e99ce75ae53dbcaf71,
title = "Comparative molecular analysis of community-associated and healthcare-associated methicillin-resistant Staphylococcus aureus isolates from children in northern Taiwan",
abstract = "From August 2004 to July 2005, 210 clinical methicillin-resistant Staphylococcus aureus (MRSA) isolates were collected prospectively from 173 children admitted to Chang Gung Children's Hospital in Taiwan. A comparative molecular analysis of the 111 community-associated (CA) isolates from 102 children and the 99 healthcare-associated (HA) isolates from 71 children was conducted. In comparison to the HA isolates (31{\%}), the CA isolates (90{\%}) were more likely to have been isolated from pus (p <5 × 10-8). For each patient with MRSA infection, only the first isolate was selected for molecular analysis. The molecular characteristics differed significantly between the CA and the HA isolates (p < 5 × 10-8). The clone characterized as sequence type (ST)59/pulsotype D (similar to USA1000)/staphylococcal chromosomal cassette (SCC) mec VT/ Panton-Valentine leukocidin (PVL)-positive accounted for 69{\%} of the CA isolates, and another clone, characterized as ST239/pulsotype. A (Hungary clone)/SCCmec III/PVL-negative, accounted for 45{\%} of the 71 HA isolates. The CA clone of ST59 also accounted for 20{\%} of the HA isolates, including 47{\%} of the 17 community-onset isolates. It was concluded that the molecular characteristics of clinical MRSA isolates from children differed significantly between the CA and the HA isolates in northern Taiwan. However, the CA clone of ST59 was also identified in a substantial proportion of HA isolates.",
keywords = "Community-associated, Healthcare-associated, Methicillin-resistant Staphylococcus aureus, Molecular characteristics, Taiwan",
author = "Huang, {Y. C.} and Ho, {C. F.} and Chen, {C. J.} and Su, {L. H.} and Tzou-Yien Lin",
year = "2008",
month = "1",
day = "1",
doi = "10.1111/j.1469-0691.2008.02115.x",
language = "English",
volume = "14",
pages = "1167--1172",
journal = "Clinical Microbiology and Infection",
issn = "1198-743X",
publisher = "Elsevier Limited",
number = "12",

}

TY - JOUR

T1 - Comparative molecular analysis of community-associated and healthcare-associated methicillin-resistant Staphylococcus aureus isolates from children in northern Taiwan

AU - Huang, Y. C.

AU - Ho, C. F.

AU - Chen, C. J.

AU - Su, L. H.

AU - Lin, Tzou-Yien

PY - 2008/1/1

Y1 - 2008/1/1

N2 - From August 2004 to July 2005, 210 clinical methicillin-resistant Staphylococcus aureus (MRSA) isolates were collected prospectively from 173 children admitted to Chang Gung Children's Hospital in Taiwan. A comparative molecular analysis of the 111 community-associated (CA) isolates from 102 children and the 99 healthcare-associated (HA) isolates from 71 children was conducted. In comparison to the HA isolates (31%), the CA isolates (90%) were more likely to have been isolated from pus (p <5 × 10-8). For each patient with MRSA infection, only the first isolate was selected for molecular analysis. The molecular characteristics differed significantly between the CA and the HA isolates (p < 5 × 10-8). The clone characterized as sequence type (ST)59/pulsotype D (similar to USA1000)/staphylococcal chromosomal cassette (SCC) mec VT/ Panton-Valentine leukocidin (PVL)-positive accounted for 69% of the CA isolates, and another clone, characterized as ST239/pulsotype. A (Hungary clone)/SCCmec III/PVL-negative, accounted for 45% of the 71 HA isolates. The CA clone of ST59 also accounted for 20% of the HA isolates, including 47% of the 17 community-onset isolates. It was concluded that the molecular characteristics of clinical MRSA isolates from children differed significantly between the CA and the HA isolates in northern Taiwan. However, the CA clone of ST59 was also identified in a substantial proportion of HA isolates.

AB - From August 2004 to July 2005, 210 clinical methicillin-resistant Staphylococcus aureus (MRSA) isolates were collected prospectively from 173 children admitted to Chang Gung Children's Hospital in Taiwan. A comparative molecular analysis of the 111 community-associated (CA) isolates from 102 children and the 99 healthcare-associated (HA) isolates from 71 children was conducted. In comparison to the HA isolates (31%), the CA isolates (90%) were more likely to have been isolated from pus (p <5 × 10-8). For each patient with MRSA infection, only the first isolate was selected for molecular analysis. The molecular characteristics differed significantly between the CA and the HA isolates (p < 5 × 10-8). The clone characterized as sequence type (ST)59/pulsotype D (similar to USA1000)/staphylococcal chromosomal cassette (SCC) mec VT/ Panton-Valentine leukocidin (PVL)-positive accounted for 69% of the CA isolates, and another clone, characterized as ST239/pulsotype. A (Hungary clone)/SCCmec III/PVL-negative, accounted for 45% of the 71 HA isolates. The CA clone of ST59 also accounted for 20% of the HA isolates, including 47% of the 17 community-onset isolates. It was concluded that the molecular characteristics of clinical MRSA isolates from children differed significantly between the CA and the HA isolates in northern Taiwan. However, the CA clone of ST59 was also identified in a substantial proportion of HA isolates.

KW - Community-associated

KW - Healthcare-associated

KW - Methicillin-resistant Staphylococcus aureus

KW - Molecular characteristics

KW - Taiwan

UR - http://www.scopus.com/inward/record.url?scp=57649205402&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=57649205402&partnerID=8YFLogxK

U2 - 10.1111/j.1469-0691.2008.02115.x

DO - 10.1111/j.1469-0691.2008.02115.x

M3 - Article

VL - 14

SP - 1167

EP - 1172

JO - Clinical Microbiology and Infection

JF - Clinical Microbiology and Infection

SN - 1198-743X

IS - 12

ER -