Comparative Genomic Analysis of Coxsackievirus A6 Strains of Different Clinical Disease Entities

Yi Jen Chen, Shih Cheng Chang, Kuo Chien Tsao, Shin Ru Shih, Shu Li Yang, Tzou Yien Lin, Yhu Chering Huang

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Background: Studies regarding coxsackievirus A6 (CVA6) infection were limited. In Taiwan, outbreaks of CVA6 occurred in 2009 and 2010, respectively, but the clinical manifestations were markedly different. We conducted a study to compare the clinical features and genomic sequence between the two years. Methodology/Principal Findings: In 2009 and 2010, 205 patients with coxsackievirus A6 (CVA6) infection were treated at Chang Gung Memorial Hospital. Detailed clinical features were obtained from 126 inpatients, 62 in 2009 and 64 in 2010. Between the inpatients in 2009 and 2010, no statistically significant difference was noted in terms of demographics, length of hospital stay and laboratory data. Significantly more patients in 2009 presented with herpangina (82%) while more patients in 2010 presented with hand-foot-mouth disease (HFMD; 67%) and skin rash beyond the typical sites for HFMD. Complete genomic sequences were determined and compared for three isolates from patients with herpangina in 2009 and three isolates from patients with HFMD in 2010. The complete sequences showed that 2009 and 2010 CVA6 isolates were indistinguishable by partial VP1 genes, but there were 5 unique nucleotide changes in 3′ UTR, and 23 out of 2201 (1%) amino acids were different. 2010 viruses underwent the largest number of amino acid changes in 3CD protein, which is the precursor of both 3C protease and 3D polymerase. Conclusions: Since 2008 in Finland, outbreaks of HFMD due to CVA6 were noted internationally. CVA6 of different genetic background may cause different clinical manifestations such as herpangina and HFMD.

Original languageEnglish
Article numbere52432
JournalPLoS One
Volume7
Issue number12
DOIs
Publication statusPublished - Dec 29 2012
Externally publishedYes

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Enterovirus
Herpangina
genomics
Amino Acids
Coxsackievirus Infections
3' Untranslated Regions
Viruses
Skin
Nucleotides
Genes
Disease Outbreaks
Inpatients
Length of Stay
Hand, Foot and Mouth Disease
Hospital Laboratories
hand, foot and mouth disease
Finland
Exanthema
Proteins
Taiwan

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Chen, Y. J., Chang, S. C., Tsao, K. C., Shih, S. R., Yang, S. L., Lin, T. Y., & Huang, Y. C. (2012). Comparative Genomic Analysis of Coxsackievirus A6 Strains of Different Clinical Disease Entities. PLoS One, 7(12), [e52432]. https://doi.org/10.1371/journal.pone.0052432

Comparative Genomic Analysis of Coxsackievirus A6 Strains of Different Clinical Disease Entities. / Chen, Yi Jen; Chang, Shih Cheng; Tsao, Kuo Chien; Shih, Shin Ru; Yang, Shu Li; Lin, Tzou Yien; Huang, Yhu Chering.

In: PLoS One, Vol. 7, No. 12, e52432, 29.12.2012.

Research output: Contribution to journalArticle

Chen, Yi Jen ; Chang, Shih Cheng ; Tsao, Kuo Chien ; Shih, Shin Ru ; Yang, Shu Li ; Lin, Tzou Yien ; Huang, Yhu Chering. / Comparative Genomic Analysis of Coxsackievirus A6 Strains of Different Clinical Disease Entities. In: PLoS One. 2012 ; Vol. 7, No. 12.
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