Comparative effect of lipoxygenase products of arachidonic acid on rat aortic smooth muscle cell migration

Junko Nakao, Toshiro Ooyama, Hideki Ito, Wen Chang Chang, Sei itsu Murota

Research output: Contribution to journalArticle

109 Citations (Scopus)

Abstract

We investigated the effects of mono-hydroxyeicosatetraenoic acids (HETEs) and N-formyl-methionyl-leucyl-phenylalanine (F-Met-Leu-Phe) on rat aortic smooth muscle cell migration in modified Boyden chambers. 12-HETE showed the most potent stimulatory effect on smooth muscle cell migration among the mono-HETEs tested. The optimal concentrations for cell migration were 3 × 10-15 and 3 × 10-13 g/ml for 12-HETE and 10-8 g/ml for 15-HETE. 5-HETE and F-Met-Leu-Phe were inactive with these cells. As 12-HETE is biosynthesized from arachidonic acid by the 12-lipoxygenase pathway in platelets and macrophages, and 15-HETE by the 15-lipoxygenase pathway in granulocytes, the present results indicate an important role for such cells in the early phase of atherosclerosis.

Original languageEnglish
Pages (from-to)339-342
Number of pages4
JournalAtherosclerosis
Volume44
Issue number3
DOIs
Publication statusPublished - 1982
Externally publishedYes

Keywords

  • Aorta
  • Arachidonic acid
  • Atherosclerosis
  • Lipoxygenase products
  • Smooth muscle cell migration

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

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