Comparative effect of lipoxygenase products of arachidonic acid on rat aortic smooth muscle cell migration

Junko Nakao, Toshiro Ooyama, Hideki Ito, Wen Chang Chang, Sei itsu Murota

Research output: Contribution to journalArticle

109 Citations (Scopus)

Abstract

We investigated the effects of mono-hydroxyeicosatetraenoic acids (HETEs) and N-formyl-methionyl-leucyl-phenylalanine (F-Met-Leu-Phe) on rat aortic smooth muscle cell migration in modified Boyden chambers. 12-HETE showed the most potent stimulatory effect on smooth muscle cell migration among the mono-HETEs tested. The optimal concentrations for cell migration were 3 × 10-15 and 3 × 10-13 g/ml for 12-HETE and 10-8 g/ml for 15-HETE. 5-HETE and F-Met-Leu-Phe were inactive with these cells. As 12-HETE is biosynthesized from arachidonic acid by the 12-lipoxygenase pathway in platelets and macrophages, and 15-HETE by the 15-lipoxygenase pathway in granulocytes, the present results indicate an important role for such cells in the early phase of atherosclerosis.

Original languageEnglish
Pages (from-to)339-342
Number of pages4
JournalAtherosclerosis
Volume44
Issue number3
DOIs
Publication statusPublished - 1982
Externally publishedYes

Fingerprint

12-Hydroxy-5,8,10,14-eicosatetraenoic Acid
Arachidonate Lipoxygenases
N-Formylmethionine Leucyl-Phenylalanine
Hydroxyeicosatetraenoic Acids
Smooth Muscle Myocytes
Cell Movement
Arachidonate 12-Lipoxygenase
Arachidonate 15-Lipoxygenase
Granulocytes
Atherosclerosis
Blood Platelets
Macrophages
15-hydroxy-5,8,11,13-eicosatetraenoic acid

Keywords

  • Aorta
  • Arachidonic acid
  • Atherosclerosis
  • Lipoxygenase products
  • Smooth muscle cell migration

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Comparative effect of lipoxygenase products of arachidonic acid on rat aortic smooth muscle cell migration. / Nakao, Junko; Ooyama, Toshiro; Ito, Hideki; Chang, Wen Chang; Murota, Sei itsu.

In: Atherosclerosis, Vol. 44, No. 3, 1982, p. 339-342.

Research output: Contribution to journalArticle

Nakao, Junko ; Ooyama, Toshiro ; Ito, Hideki ; Chang, Wen Chang ; Murota, Sei itsu. / Comparative effect of lipoxygenase products of arachidonic acid on rat aortic smooth muscle cell migration. In: Atherosclerosis. 1982 ; Vol. 44, No. 3. pp. 339-342.
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