Comparative anti-inflammatory characterization of wild fruiting body, liquid-state fermentation, and solid-state culture of Taiwanofungus camphoratus in microglia and the mechanism of its action

Der Zen Liu, Hong Jen Liang, Chien Ho Chen, Ching Hua Su, Zong-Huei Li, Chun Ting Huang, Wen Chi Hou, Shyr Yi Lin, Wen-Bin Zhong, Pei Jung Lin, Ling Fang Hung, Yu Chih Liang

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

Taiwanofungus camphoratus (syn. Antrodia camphorata), a medicinal mushroom in Taiwan, is reputed to provide several therapeutic benefits, but the wild fruiting body is very rare. In this study, we used Taiwanofungus camphoratus extracts from wild fruiting bodies and two types of artificial cultivation (solid-state culture and liquid-state fermentation) to examine their anti-inflammatory effects in microglia cells and their possible roles in protection against neurodegenerative diseases. First, EOC13.31 microglia was treated with various kinds of Taiwanofungus camphoratus extracts and lipopolysaccharide (LPS) and interferon-γ (IFN-γ) to evaluate the iNOS expression. Western blot and RT-PCR analysis showed that among the various kinds of extracts from wild fruiting bodies, methanol extracts were the most potent inhibitors of iNOS expression. Secondly, the potency of methanol extracts could be ranked as follows: extracts of wild fruiting body > solid-state culture > liquid-state fermentation. To clarify the mechanisms involved, methanol extracts from fruiting body were found to inhibit the phosphorylation of extracellular signal-regulated protein kinases (ERK), c-Jun NH2-terminal protein kinases (JNK) and signal transducer and activator of transcription-1 (STAT-1) induced by LPS/IFN-γ. Methanol extracts from fruiting body also inhibited NF-κB activation through the prevention of inhibitor κB (IκB) degradation. Moreover, methanol extracts from wild fruiting body inhibited both the iNOS and cyclooxygenase-2 (COX-2) expression induced by β-amyloid in microglia in a dose-dependent manner. In an animal model, we confirmed that methanol extracts from fruiting bodies were able to suppress ear edema, indicating that they have anti-inflammatory activity in vivo. These results suggest that Taiwanofungus camphoratus exhibits an anti-inflammatory activity that might contribute to the prevention of neurodegenerative diseases.

Original languageEnglish
Pages (from-to)45-53
Number of pages9
JournalJournal of Ethnopharmacology
Volume113
Issue number1
DOIs
Publication statusPublished - Aug 15 2007

Fingerprint

Microglia
Fermentation
Methanol
Anti-Inflammatory Agents
Neurodegenerative Diseases
Protein Kinases
Interferons
Lipopolysaccharides
Antrodia
STAT1 Transcription Factor
Somatotypes
JNK Mitogen-Activated Protein Kinases
Agaricales
Extracellular Signal-Regulated MAP Kinases
Cyclooxygenase 2
Taiwan
Amyloid
Ear
Edema
Animal Models

Keywords

  • Inflammation
  • iNOS
  • Microglia
  • Taiwanofungus camphoratus

ASJC Scopus subject areas

  • Pharmacology

Cite this

Comparative anti-inflammatory characterization of wild fruiting body, liquid-state fermentation, and solid-state culture of Taiwanofungus camphoratus in microglia and the mechanism of its action. / Liu, Der Zen; Liang, Hong Jen; Chen, Chien Ho; Su, Ching Hua; Li, Zong-Huei; Huang, Chun Ting; Hou, Wen Chi; Lin, Shyr Yi; Zhong, Wen-Bin; Lin, Pei Jung; Hung, Ling Fang; Liang, Yu Chih.

In: Journal of Ethnopharmacology, Vol. 113, No. 1, 15.08.2007, p. 45-53.

Research output: Contribution to journalArticle

@article{9f2c10933abd4995932cfee07c39a98b,
title = "Comparative anti-inflammatory characterization of wild fruiting body, liquid-state fermentation, and solid-state culture of Taiwanofungus camphoratus in microglia and the mechanism of its action",
abstract = "Taiwanofungus camphoratus (syn. Antrodia camphorata), a medicinal mushroom in Taiwan, is reputed to provide several therapeutic benefits, but the wild fruiting body is very rare. In this study, we used Taiwanofungus camphoratus extracts from wild fruiting bodies and two types of artificial cultivation (solid-state culture and liquid-state fermentation) to examine their anti-inflammatory effects in microglia cells and their possible roles in protection against neurodegenerative diseases. First, EOC13.31 microglia was treated with various kinds of Taiwanofungus camphoratus extracts and lipopolysaccharide (LPS) and interferon-γ (IFN-γ) to evaluate the iNOS expression. Western blot and RT-PCR analysis showed that among the various kinds of extracts from wild fruiting bodies, methanol extracts were the most potent inhibitors of iNOS expression. Secondly, the potency of methanol extracts could be ranked as follows: extracts of wild fruiting body > solid-state culture > liquid-state fermentation. To clarify the mechanisms involved, methanol extracts from fruiting body were found to inhibit the phosphorylation of extracellular signal-regulated protein kinases (ERK), c-Jun NH2-terminal protein kinases (JNK) and signal transducer and activator of transcription-1 (STAT-1) induced by LPS/IFN-γ. Methanol extracts from fruiting body also inhibited NF-κB activation through the prevention of inhibitor κB (IκB) degradation. Moreover, methanol extracts from wild fruiting body inhibited both the iNOS and cyclooxygenase-2 (COX-2) expression induced by β-amyloid in microglia in a dose-dependent manner. In an animal model, we confirmed that methanol extracts from fruiting bodies were able to suppress ear edema, indicating that they have anti-inflammatory activity in vivo. These results suggest that Taiwanofungus camphoratus exhibits an anti-inflammatory activity that might contribute to the prevention of neurodegenerative diseases.",
keywords = "Inflammation, iNOS, Microglia, Taiwanofungus camphoratus",
author = "Liu, {Der Zen} and Liang, {Hong Jen} and Chen, {Chien Ho} and Su, {Ching Hua} and Zong-Huei Li and Huang, {Chun Ting} and Hou, {Wen Chi} and Lin, {Shyr Yi} and Wen-Bin Zhong and Lin, {Pei Jung} and Hung, {Ling Fang} and Liang, {Yu Chih}",
year = "2007",
month = "8",
day = "15",
doi = "10.1016/j.jep.2007.03.037",
language = "English",
volume = "113",
pages = "45--53",
journal = "Journal of Ethnopharmacology",
issn = "0378-8741",
publisher = "Elsevier Ireland Ltd",
number = "1",

}

TY - JOUR

T1 - Comparative anti-inflammatory characterization of wild fruiting body, liquid-state fermentation, and solid-state culture of Taiwanofungus camphoratus in microglia and the mechanism of its action

AU - Liu, Der Zen

AU - Liang, Hong Jen

AU - Chen, Chien Ho

AU - Su, Ching Hua

AU - Li, Zong-Huei

AU - Huang, Chun Ting

AU - Hou, Wen Chi

AU - Lin, Shyr Yi

AU - Zhong, Wen-Bin

AU - Lin, Pei Jung

AU - Hung, Ling Fang

AU - Liang, Yu Chih

PY - 2007/8/15

Y1 - 2007/8/15

N2 - Taiwanofungus camphoratus (syn. Antrodia camphorata), a medicinal mushroom in Taiwan, is reputed to provide several therapeutic benefits, but the wild fruiting body is very rare. In this study, we used Taiwanofungus camphoratus extracts from wild fruiting bodies and two types of artificial cultivation (solid-state culture and liquid-state fermentation) to examine their anti-inflammatory effects in microglia cells and their possible roles in protection against neurodegenerative diseases. First, EOC13.31 microglia was treated with various kinds of Taiwanofungus camphoratus extracts and lipopolysaccharide (LPS) and interferon-γ (IFN-γ) to evaluate the iNOS expression. Western blot and RT-PCR analysis showed that among the various kinds of extracts from wild fruiting bodies, methanol extracts were the most potent inhibitors of iNOS expression. Secondly, the potency of methanol extracts could be ranked as follows: extracts of wild fruiting body > solid-state culture > liquid-state fermentation. To clarify the mechanisms involved, methanol extracts from fruiting body were found to inhibit the phosphorylation of extracellular signal-regulated protein kinases (ERK), c-Jun NH2-terminal protein kinases (JNK) and signal transducer and activator of transcription-1 (STAT-1) induced by LPS/IFN-γ. Methanol extracts from fruiting body also inhibited NF-κB activation through the prevention of inhibitor κB (IκB) degradation. Moreover, methanol extracts from wild fruiting body inhibited both the iNOS and cyclooxygenase-2 (COX-2) expression induced by β-amyloid in microglia in a dose-dependent manner. In an animal model, we confirmed that methanol extracts from fruiting bodies were able to suppress ear edema, indicating that they have anti-inflammatory activity in vivo. These results suggest that Taiwanofungus camphoratus exhibits an anti-inflammatory activity that might contribute to the prevention of neurodegenerative diseases.

AB - Taiwanofungus camphoratus (syn. Antrodia camphorata), a medicinal mushroom in Taiwan, is reputed to provide several therapeutic benefits, but the wild fruiting body is very rare. In this study, we used Taiwanofungus camphoratus extracts from wild fruiting bodies and two types of artificial cultivation (solid-state culture and liquid-state fermentation) to examine their anti-inflammatory effects in microglia cells and their possible roles in protection against neurodegenerative diseases. First, EOC13.31 microglia was treated with various kinds of Taiwanofungus camphoratus extracts and lipopolysaccharide (LPS) and interferon-γ (IFN-γ) to evaluate the iNOS expression. Western blot and RT-PCR analysis showed that among the various kinds of extracts from wild fruiting bodies, methanol extracts were the most potent inhibitors of iNOS expression. Secondly, the potency of methanol extracts could be ranked as follows: extracts of wild fruiting body > solid-state culture > liquid-state fermentation. To clarify the mechanisms involved, methanol extracts from fruiting body were found to inhibit the phosphorylation of extracellular signal-regulated protein kinases (ERK), c-Jun NH2-terminal protein kinases (JNK) and signal transducer and activator of transcription-1 (STAT-1) induced by LPS/IFN-γ. Methanol extracts from fruiting body also inhibited NF-κB activation through the prevention of inhibitor κB (IκB) degradation. Moreover, methanol extracts from wild fruiting body inhibited both the iNOS and cyclooxygenase-2 (COX-2) expression induced by β-amyloid in microglia in a dose-dependent manner. In an animal model, we confirmed that methanol extracts from fruiting bodies were able to suppress ear edema, indicating that they have anti-inflammatory activity in vivo. These results suggest that Taiwanofungus camphoratus exhibits an anti-inflammatory activity that might contribute to the prevention of neurodegenerative diseases.

KW - Inflammation

KW - iNOS

KW - Microglia

KW - Taiwanofungus camphoratus

UR - http://www.scopus.com/inward/record.url?scp=34447545716&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34447545716&partnerID=8YFLogxK

U2 - 10.1016/j.jep.2007.03.037

DO - 10.1016/j.jep.2007.03.037

M3 - Article

C2 - 17590297

AN - SCOPUS:34447545716

VL - 113

SP - 45

EP - 53

JO - Journal of Ethnopharmacology

JF - Journal of Ethnopharmacology

SN - 0378-8741

IS - 1

ER -