Combining the observation of cell morphology with the evaluation of key inflammatory mediators to assess the anti-inflammatory effects of geranyl flavonoid derivatives in breadfruit

Jer An Lin, Chi Hao Wu, Song Chwan Fang, Gow Chin Yen

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Ligation of the receptor for advanced glycation end products (RAGE) is critical for monocyte activation involved in diabetic inflammation. In this study, the effects of the geranyl flavonoid derivatives (6-geranyl-7,4′- dihydroxyflavanone, AC-1; 4,2′,4′-trihydroxy-3′-[6-hydroxy-3, 7-dimethyl-2(E),7-octadienyl]chalcone, AC-2; 3,4,2′,4′-tetrahydroxy- 3′-geranyldihydrochalcone, AC-3; 4,2′,4′-trihydroxy-5′- geranyldihydrochalcone, AC-4) isolated from the fruit of Artocarpus communis (breadfruit) on the human THP-1 monocyte (THP-1) activation stimulated by S100B, a ligand of RAGE, were evaluated. The morphology of S100B-induced THP-1, which may be essential for the elucidation of the functional role of S100B in monocyte activation was investigated. We also directly demonstrated that S100B-induced THP-1 exhibited the morphological characteristics of inflammation, which were inhibited by the addition of AC-2, AC-3 or AC-4. Moreover, AC-2, AC-3 or AC-4 inhibited S100B-induced ROS generation, mRNA expression of inflammatory mediators (COX-2, TNF-α, IL-6 and RAGE) and IL-6 secretion. Thus, geranyl flavonoid derivatives of breadfruit may have potent implications to prevent diabetic inflammation.

Original languageEnglish
Pages (from-to)2118-2125
Number of pages8
JournalFood Chemistry
Volume132
Issue number4
DOIs
Publication statusPublished - Jun 15 2012
Externally publishedYes

Keywords

  • Artocarpus communis
  • Geranyl flavonoid derivatives
  • Inflammation
  • S100B
  • THP-1

ASJC Scopus subject areas

  • Food Science
  • Analytical Chemistry

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