Combined effects of maternal inflammation and neonatal hyperoxia on lung fibrosis and RAGE expression in newborn rats

Chien-Ling Su, Hsiu Chu Chou, Liang Ti Huang, Tsu Fu Yeh, Chung Ming Chen

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Background:Receptors for advanced glycation end products (RAGE) have been implicated in fibrotic processes. We hypothesized that lung fibrosis induced by maternal lipopolysaccharide (LPS)-mediated inflammation and neonatal hyperoxia involves RAGE in newborn rats.Methods:Pregnant Sprague-Dawley rats received intraperitoneal injections of LPS or normal saline (NS) on 20 and 21 d of gestation. The pups were reared in room air (RA) or an O 2-enrich atmosphere (O 2), creating the four study groups, NS + RA, NS + O 2, LPS + RA, and LPS + O 2. The O 2 treatment was >95% O 2 for 7 d, followed by 60% O 2 for 14 d.Results:Rat pups born to LPS-injected dams exhibited significantly higher lung interferon-γ and interleukin-1β (IL-1β) on postnatal day 7 than the pups born to NS-injected dams. Rat pups reared in hyperoxia expressed higher lung IL-10 on postnatal day 7, compared with the RA-reared pups. The LPS + O 2 group had significantly higher total collagen and transforming growth factor-β1 on postnatal days 7 and 21 than the NS+RA group. RAGE mRNA and sRAGE protein expression were significantly lower in the LPS + O 2 group on postnatal day 7 than the NS+RA group.Conclusion:RAGE may be involved in the pathogenesis of lung fibrosis induced by maternal systemic inflammation and postnatal hyperoxia in rat neonates.

Original languageEnglish
Pages (from-to)273-280
Number of pages8
JournalPediatric Research
Volume75
Issue number2
DOIs
Publication statusPublished - Feb 2014

Fingerprint

Hyperoxia
Lipopolysaccharides
Fibrosis
Mothers
Inflammation
Air
Lung
Transforming Growth Factors
Advanced Glycosylation End Product-Specific Receptor
Intraperitoneal Injections
Atmosphere
Interleukin-1
Interleukin-10
Interferons
Sprague Dawley Rats
Collagen
Pregnancy
Messenger RNA

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health

Cite this

Combined effects of maternal inflammation and neonatal hyperoxia on lung fibrosis and RAGE expression in newborn rats. / Su, Chien-Ling; Chou, Hsiu Chu; Huang, Liang Ti; Yeh, Tsu Fu; Chen, Chung Ming.

In: Pediatric Research, Vol. 75, No. 2, 02.2014, p. 273-280.

Research output: Contribution to journalArticle

@article{e2101e547b49419494b592dd434faed6,
title = "Combined effects of maternal inflammation and neonatal hyperoxia on lung fibrosis and RAGE expression in newborn rats",
abstract = "Background:Receptors for advanced glycation end products (RAGE) have been implicated in fibrotic processes. We hypothesized that lung fibrosis induced by maternal lipopolysaccharide (LPS)-mediated inflammation and neonatal hyperoxia involves RAGE in newborn rats.Methods:Pregnant Sprague-Dawley rats received intraperitoneal injections of LPS or normal saline (NS) on 20 and 21 d of gestation. The pups were reared in room air (RA) or an O 2-enrich atmosphere (O 2), creating the four study groups, NS + RA, NS + O 2, LPS + RA, and LPS + O 2. The O 2 treatment was >95{\%} O 2 for 7 d, followed by 60{\%} O 2 for 14 d.Results:Rat pups born to LPS-injected dams exhibited significantly higher lung interferon-γ and interleukin-1β (IL-1β) on postnatal day 7 than the pups born to NS-injected dams. Rat pups reared in hyperoxia expressed higher lung IL-10 on postnatal day 7, compared with the RA-reared pups. The LPS + O 2 group had significantly higher total collagen and transforming growth factor-β1 on postnatal days 7 and 21 than the NS+RA group. RAGE mRNA and sRAGE protein expression were significantly lower in the LPS + O 2 group on postnatal day 7 than the NS+RA group.Conclusion:RAGE may be involved in the pathogenesis of lung fibrosis induced by maternal systemic inflammation and postnatal hyperoxia in rat neonates.",
author = "Chien-Ling Su and Chou, {Hsiu Chu} and Huang, {Liang Ti} and Yeh, {Tsu Fu} and Chen, {Chung Ming}",
year = "2014",
month = "2",
doi = "10.1038/pr.2013.222",
language = "English",
volume = "75",
pages = "273--280",
journal = "Pediatric Research",
issn = "0031-3998",
publisher = "Lippincott Williams and Wilkins",
number = "2",

}

TY - JOUR

T1 - Combined effects of maternal inflammation and neonatal hyperoxia on lung fibrosis and RAGE expression in newborn rats

AU - Su, Chien-Ling

AU - Chou, Hsiu Chu

AU - Huang, Liang Ti

AU - Yeh, Tsu Fu

AU - Chen, Chung Ming

PY - 2014/2

Y1 - 2014/2

N2 - Background:Receptors for advanced glycation end products (RAGE) have been implicated in fibrotic processes. We hypothesized that lung fibrosis induced by maternal lipopolysaccharide (LPS)-mediated inflammation and neonatal hyperoxia involves RAGE in newborn rats.Methods:Pregnant Sprague-Dawley rats received intraperitoneal injections of LPS or normal saline (NS) on 20 and 21 d of gestation. The pups were reared in room air (RA) or an O 2-enrich atmosphere (O 2), creating the four study groups, NS + RA, NS + O 2, LPS + RA, and LPS + O 2. The O 2 treatment was >95% O 2 for 7 d, followed by 60% O 2 for 14 d.Results:Rat pups born to LPS-injected dams exhibited significantly higher lung interferon-γ and interleukin-1β (IL-1β) on postnatal day 7 than the pups born to NS-injected dams. Rat pups reared in hyperoxia expressed higher lung IL-10 on postnatal day 7, compared with the RA-reared pups. The LPS + O 2 group had significantly higher total collagen and transforming growth factor-β1 on postnatal days 7 and 21 than the NS+RA group. RAGE mRNA and sRAGE protein expression were significantly lower in the LPS + O 2 group on postnatal day 7 than the NS+RA group.Conclusion:RAGE may be involved in the pathogenesis of lung fibrosis induced by maternal systemic inflammation and postnatal hyperoxia in rat neonates.

AB - Background:Receptors for advanced glycation end products (RAGE) have been implicated in fibrotic processes. We hypothesized that lung fibrosis induced by maternal lipopolysaccharide (LPS)-mediated inflammation and neonatal hyperoxia involves RAGE in newborn rats.Methods:Pregnant Sprague-Dawley rats received intraperitoneal injections of LPS or normal saline (NS) on 20 and 21 d of gestation. The pups were reared in room air (RA) or an O 2-enrich atmosphere (O 2), creating the four study groups, NS + RA, NS + O 2, LPS + RA, and LPS + O 2. The O 2 treatment was >95% O 2 for 7 d, followed by 60% O 2 for 14 d.Results:Rat pups born to LPS-injected dams exhibited significantly higher lung interferon-γ and interleukin-1β (IL-1β) on postnatal day 7 than the pups born to NS-injected dams. Rat pups reared in hyperoxia expressed higher lung IL-10 on postnatal day 7, compared with the RA-reared pups. The LPS + O 2 group had significantly higher total collagen and transforming growth factor-β1 on postnatal days 7 and 21 than the NS+RA group. RAGE mRNA and sRAGE protein expression were significantly lower in the LPS + O 2 group on postnatal day 7 than the NS+RA group.Conclusion:RAGE may be involved in the pathogenesis of lung fibrosis induced by maternal systemic inflammation and postnatal hyperoxia in rat neonates.

UR - http://www.scopus.com/inward/record.url?scp=84894213012&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84894213012&partnerID=8YFLogxK

U2 - 10.1038/pr.2013.222

DO - 10.1038/pr.2013.222

M3 - Article

VL - 75

SP - 273

EP - 280

JO - Pediatric Research

JF - Pediatric Research

SN - 0031-3998

IS - 2

ER -