Combined determination of circulating miR-196a and miR-196b levels produces high sensitivity and specificity for early detection of oral cancer

Ya Ching Lu, Joseph Tung Chieh Chang, Yu Chen Huang, Chi Che Huang, Wen Ho Chen, Li Yu Lee, Bing Shen Huang, Yin Ju Chen, Hsiao Fang Li, Ann Joy Cheng

Research output: Contribution to journalArticle

37 Citations (Scopus)


Objectives: The aim of this study was to determine whether the oncogenic microRNA family members miR-196a and miR-196b can be circulating biomarkers for the early detection of oral cancer. Design and methods: To determine the stability of circulating miRNA, the blood sample was aliquot and stored at different temperature conditions for analysis. To assess the diagnostic efficacy, we determined the levels of miR-196s in plasma samples, including 53 from healthy individuals, 16 from pre-cancer patients, and 90 from oral cancer patients. Results: In general, circulating miRNA was very stable when storing plasma samples at -20. °C or below. In clinical study, both circulating miR-196a and miR-196b were substantially up-regulated in patients with oral pre-cancer lesions (5.9- and 14.8-fold, respectively; P

Original languageEnglish
Pages (from-to)115-121
Number of pages7
JournalClinical Biochemistry
Issue number3
Publication statusPublished - Feb 1 2015
Externally publishedYes



  • Circulating miRNA
  • Early detection
  • MiR-196a
  • MiR-196b
  • Oral cancer

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Medicine(all)

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