Colon mustard oil instillation induced cross-organ reflex sensitization on the pelvic-urethra reflex activity in rats

Hsien Yu Peng, Gin Den Chen, Kwong Chung Tung, Cheng Yuan Lai, Ming Chun Hsien, Chun Hsien Chiu, Hsiao Ting Lu, Jiuan Miaw Liao, Shin Da Lee, Tzer Bin Lin

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

We investigated the participation of cyclin-dependent kinase-5 (Cdk5)-mediated N-methyl-d-aspartate receptor (NMDAR) NR2B subunit phosphorylation in cross-organ reflex sensitization caused by colon irritation. The external urethral sphincter electromyogram (EUSE) reflex activity evoked by the pelvic afferent nerve test stimulation (TS, 1 stimulation/30 s) and protein expression in the spinal cord and dorsal root ganglion tissue (T13-L2 and L6-S2 ipsilateral to the stimulation) in response to colon mustard oil (MO) instillation were tested in anesthetized rats. When compared with a baseline reflex activity with a single action potential evoked by the TS before the administration of test agents, MO instillation into the descending colon sensitized the evoked activity characterized by elongated firing in the reflex activity in association with increased protein levels of Cdk5, PSD95, and phosphorylated NR2B (pNR2B) but not of total NR2B (tNR2B) in the spinal cord tissue. Both cross-organ reflex sensitization and increments in protein expression were reversed by intra-colonic pretreatments with ruthenium red (a non-selective transient receptor potential vanilloid, TRPV, antagonist), capsaizepine (a TRPV1-selective antagonist), lidocaine (a nerve conduction blocker) as well as by the intra-thecal pretreatment with APV (a NRMDR antagonist) Co-101244 (a NR2B-selective antagonist) and roscovitine (a Cdk5 antagonist). Moreover, compared with the control group, both the increase in pNR2B and the cross-organ reflex sensitization were attenuated in the si-RNA of NR2B rats. All these results suggested that Cdk-dependent NMDAR NR2B subunit phosphorylation mediates the development of cross-organ pelvic-urethra reflex sensitization caused by acute colon irritation which could possibly underlie the high concurrence of pelvic pain syndrome with irritable bowel syndrome.

Original languageEnglish
Pages (from-to)75-88
Number of pages14
JournalPain
Volume142
Issue number1-2
DOIs
Publication statusPublished - Mar 2009
Externally publishedYes

Fingerprint

Urethra
Reflex
Colon
Cyclin-Dependent Kinase 5
Spinal Cord
Phosphorylation
Descending Colon
TRPV Cation Channels
Ruthenium Red
Proteins
Pelvic Pain
Spinal Nerve Roots
Irritable Bowel Syndrome
Neural Conduction
Spinal Ganglia
Electromyography
Lidocaine
mustard oil
Action Potentials
RNA

Keywords

  • Convergent pathway
  • Glutamate
  • Irritable bowel syndrome
  • Pelvic pain
  • Serine kinase

ASJC Scopus subject areas

  • Clinical Neurology
  • Anesthesiology and Pain Medicine
  • Neurology
  • Pharmacology

Cite this

Colon mustard oil instillation induced cross-organ reflex sensitization on the pelvic-urethra reflex activity in rats. / Peng, Hsien Yu; Chen, Gin Den; Tung, Kwong Chung; Lai, Cheng Yuan; Hsien, Ming Chun; Chiu, Chun Hsien; Lu, Hsiao Ting; Liao, Jiuan Miaw; Lee, Shin Da; Lin, Tzer Bin.

In: Pain, Vol. 142, No. 1-2, 03.2009, p. 75-88.

Research output: Contribution to journalArticle

Peng, HY, Chen, GD, Tung, KC, Lai, CY, Hsien, MC, Chiu, CH, Lu, HT, Liao, JM, Lee, SD & Lin, TB 2009, 'Colon mustard oil instillation induced cross-organ reflex sensitization on the pelvic-urethra reflex activity in rats', Pain, vol. 142, no. 1-2, pp. 75-88. https://doi.org/10.1016/j.pain.2008.11.017
Peng, Hsien Yu ; Chen, Gin Den ; Tung, Kwong Chung ; Lai, Cheng Yuan ; Hsien, Ming Chun ; Chiu, Chun Hsien ; Lu, Hsiao Ting ; Liao, Jiuan Miaw ; Lee, Shin Da ; Lin, Tzer Bin. / Colon mustard oil instillation induced cross-organ reflex sensitization on the pelvic-urethra reflex activity in rats. In: Pain. 2009 ; Vol. 142, No. 1-2. pp. 75-88.
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