Collagen VIII is expressed by vascular smooth muscle cells in response to vascular injury

Nicholas E S Sibinga, Lauren C. Foster, Chung Ming Hsieh, Mark A. Perrella, Wen Sen Lee, Wilson O. Endege, E. Helene Sage, Mu En Lee, Edgar Haber

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

To identify genes involved in vascular remodeling, we applied differential mRNA display analysis to the rat carotid artery balloon injury model. One polymerase chain reaction product showing increased expression at days 2 to 14 after vascular injury was nearly identical to the mouse α1 chain of type VIII collagen, a heterotrimeric short-chain collagen of uncertain function expressed by a limited number of cell types. By Northern analysis, expression of both chains of the type VIII collagen heterotrimer increased: collagen α1 (VIII) mRNA expression was almost 4-fold higher than control by 7 days after vascular injury, and collagen α2 (VIII) mRNA expression reached a maximum of almost 6-fold above baseline by 3 days after injury. By immunohistochemical analysis, type VIII collagen expression increased in the media and neointima in a localized pattern consistent with the distribution of activated dedifferentiated vascular smooth muscle cells (VSMCs). Cultured VSMCs expressed higher levels of type VIII collagen in response to serum and growth factors, notably platelet-derived growth factor (PDGF)-BB. VSMCs adhered significantly less to type VIII collagen than to type I coIlagen substrata and showed greater PDGF-BB-stimulated migration (by 2.2-fold) on type VIII collagen than on type I collagen. We hypothesize that increased expression of type VIII collagen by VSMCs after arterial injury may contribute to vascular remodeling through the promotion of VSMC migration.

Original languageEnglish
Pages (from-to)532-541
Number of pages10
JournalCirculation Research
Volume80
Issue number4
Publication statusPublished - 1997
Externally publishedYes

Fingerprint

Collagen Type VIII
Vascular System Injuries
Vascular Smooth Muscle
Smooth Muscle Myocytes
Collagen
Collagen Type I
Non-Fibrillar Collagens
Carotid Artery Injuries
Neointima
Messenger RNA
Wounds and Injuries
Gene Expression Profiling
Cell Movement
Intercellular Signaling Peptides and Proteins
Cell Count
Polymerase Chain Reaction
Serum
Genes

Keywords

  • artery
  • cardiovascular disease
  • cell movement
  • extracellular matrix
  • gene expression

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

Sibinga, N. E. S., Foster, L. C., Hsieh, C. M., Perrella, M. A., Lee, W. S., Endege, W. O., ... Haber, E. (1997). Collagen VIII is expressed by vascular smooth muscle cells in response to vascular injury. Circulation Research, 80(4), 532-541.

Collagen VIII is expressed by vascular smooth muscle cells in response to vascular injury. / Sibinga, Nicholas E S; Foster, Lauren C.; Hsieh, Chung Ming; Perrella, Mark A.; Lee, Wen Sen; Endege, Wilson O.; Sage, E. Helene; Lee, Mu En; Haber, Edgar.

In: Circulation Research, Vol. 80, No. 4, 1997, p. 532-541.

Research output: Contribution to journalArticle

Sibinga, NES, Foster, LC, Hsieh, CM, Perrella, MA, Lee, WS, Endege, WO, Sage, EH, Lee, ME & Haber, E 1997, 'Collagen VIII is expressed by vascular smooth muscle cells in response to vascular injury', Circulation Research, vol. 80, no. 4, pp. 532-541.
Sibinga NES, Foster LC, Hsieh CM, Perrella MA, Lee WS, Endege WO et al. Collagen VIII is expressed by vascular smooth muscle cells in response to vascular injury. Circulation Research. 1997;80(4):532-541.
Sibinga, Nicholas E S ; Foster, Lauren C. ; Hsieh, Chung Ming ; Perrella, Mark A. ; Lee, Wen Sen ; Endege, Wilson O. ; Sage, E. Helene ; Lee, Mu En ; Haber, Edgar. / Collagen VIII is expressed by vascular smooth muscle cells in response to vascular injury. In: Circulation Research. 1997 ; Vol. 80, No. 4. pp. 532-541.
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