Abstract
Human bone stromal cells, after three-dimensional coculture with human prostate cancer (PCa) cells in vitro, underwent permanent cytogenetic and gene expression changes with reactive oxygen species serving as mediators. The evolved stromal cells are highly inductive of human PCa growth in mice, and expressed increased levels of extracellular matrix (versican and tenascin) and chemokine (BDFN, CCL5, CXCL5, and CXCL16) genes. These genes were validated in clinical tissue and/or serum specimens and could be the predictors for invasive and bone metastatic PCa. These results, combined with our previous observations, support the concept of permanent genetic and behavioral changes of PCa epithelial cells after being either cocultured with prostate or bone stromal cells as three-dimensional prostate organoids or grown as tumor xenografts in mice. These observations collectively suggest coevolution of cancer and stromal cells occurred under three-dimensional growth condition, which ultimately accelerates cancer growth andmetastasis.
| Original language | English |
|---|---|
| Pages (from-to) | 9996-10003 |
| Number of pages | 8 |
| Journal | Cancer Research |
| Volume | 68 |
| Issue number | 23 |
| DOIs | |
| Publication status | Published - Dec 1 2008 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Cancer Research
- Oncology
Cite this
Coevolution of prostate cancer and bone stroma in three-dimensional coculture : Implications for cancer growth and metastasis. / Sung, Shian Ying; Hsieh, Chia Ling; Law, Andrew; Zhau, Haiyen E.; Pathak, Sen; Multani, Asha S.; Lim, Sharon; Coleman, Ilsa M.; Wu, Li Chin; Figg, William D.; Dahut, William L.; Nelson, Peter; Lee, Jae K.; Amin, Mahul B.; Lyles, Robert; Johnstone, Peter A J; Marshall, Fray F.; Chung, Leland W K.
In: Cancer Research, Vol. 68, No. 23, 01.12.2008, p. 9996-10003.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Coevolution of prostate cancer and bone stroma in three-dimensional coculture
T2 - Implications for cancer growth and metastasis
AU - Sung, Shian Ying
AU - Hsieh, Chia Ling
AU - Law, Andrew
AU - Zhau, Haiyen E.
AU - Pathak, Sen
AU - Multani, Asha S.
AU - Lim, Sharon
AU - Coleman, Ilsa M.
AU - Wu, Li Chin
AU - Figg, William D.
AU - Dahut, William L.
AU - Nelson, Peter
AU - Lee, Jae K.
AU - Amin, Mahul B.
AU - Lyles, Robert
AU - Johnstone, Peter A J
AU - Marshall, Fray F.
AU - Chung, Leland W K
PY - 2008/12/1
Y1 - 2008/12/1
N2 - Human bone stromal cells, after three-dimensional coculture with human prostate cancer (PCa) cells in vitro, underwent permanent cytogenetic and gene expression changes with reactive oxygen species serving as mediators. The evolved stromal cells are highly inductive of human PCa growth in mice, and expressed increased levels of extracellular matrix (versican and tenascin) and chemokine (BDFN, CCL5, CXCL5, and CXCL16) genes. These genes were validated in clinical tissue and/or serum specimens and could be the predictors for invasive and bone metastatic PCa. These results, combined with our previous observations, support the concept of permanent genetic and behavioral changes of PCa epithelial cells after being either cocultured with prostate or bone stromal cells as three-dimensional prostate organoids or grown as tumor xenografts in mice. These observations collectively suggest coevolution of cancer and stromal cells occurred under three-dimensional growth condition, which ultimately accelerates cancer growth andmetastasis.
AB - Human bone stromal cells, after three-dimensional coculture with human prostate cancer (PCa) cells in vitro, underwent permanent cytogenetic and gene expression changes with reactive oxygen species serving as mediators. The evolved stromal cells are highly inductive of human PCa growth in mice, and expressed increased levels of extracellular matrix (versican and tenascin) and chemokine (BDFN, CCL5, CXCL5, and CXCL16) genes. These genes were validated in clinical tissue and/or serum specimens and could be the predictors for invasive and bone metastatic PCa. These results, combined with our previous observations, support the concept of permanent genetic and behavioral changes of PCa epithelial cells after being either cocultured with prostate or bone stromal cells as three-dimensional prostate organoids or grown as tumor xenografts in mice. These observations collectively suggest coevolution of cancer and stromal cells occurred under three-dimensional growth condition, which ultimately accelerates cancer growth andmetastasis.
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UR - http://www.scopus.com/inward/citedby.url?scp=57149112529&partnerID=8YFLogxK
U2 - 10.1158/0008-5472.CAN-08-2492
DO - 10.1158/0008-5472.CAN-08-2492
M3 - Article
VL - 68
SP - 9996
EP - 10003
JO - Cancer Research
JF - Cancer Research
SN - 0008-5472
IS - 23
ER -