Coenzyme Q10 Attenuates High Glucose-Induced Endothelial Progenitor Cell Dysfunction through AMP-Activated Protein Kinase Pathways

Hsiao Ya Tsai, Chih Pei Lin, Po Hsun Huang, Szu Yuan Li, Jia Shiong Chen, Feng Yen Lin, Jaw Wen Chen, Shing Jong Lin

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18 Citations (Scopus)

Abstract

Coenzyme Q10 (CoQ10), an antiapoptosis enzyme, is stored in the mitochondria of cells. We investigated whether CoQ10 can attenuate high glucose-induced endothelial progenitor cell (EPC) apoptosis and clarified its mechanism. EPCs were incubated with normal glucose (5 mM) or high glucose (25 mM) enviroment for 3 days, followed by treatment with CoQ10 (10 M) for 24 hr. Cell proliferation, nitric oxide (NO) production, and JC-1 assay were examined. The specific signal pathways of AMP-activated protein kinase (AMPK), eNOS/Akt, and heme oxygenase-1 (HO-1) were also assessed. High glucose reduced EPC functional activities, including proliferation and migration. Additionally, Akt/eNOS activity and NO production were downregulated in high glucose-stimulated EPCs. Administration of CoQ10 ameliorated high glucose-induced EPC apoptosis, including downregulation of caspase 3, upregulation of Bcl-2, and increase in mitochondrial membrane potential. Furthermore, treatment with CoQ10 reduced reactive oxygen species, enhanced eNOS/Akt activity, and increased HO-1 expression in high glucose-treated EPCs. These effects were negated by administration of AMPK inhibitor. Transplantation of CoQ10-treated EPCs under high glucose conditions into ischemic hindlimbs improved blood flow recovery. CoQ10 reduced high glucose-induced EPC apoptosis and dysfunction through upregulation of eNOS, HO-1 through the AMPK pathway. Our findings provide a potential treatment strategy targeting dysfunctional EPC in diabetic patients.

Original languageEnglish
Article number6384759
JournalJournal of Diabetes Research
Volume2016
DOIs
Publication statusPublished - 2016

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coenzyme Q10
AMP-Activated Protein Kinases
Glucose
Heme Oxygenase-1
Apoptosis
Nitric Oxide
Up-Regulation
Down-Regulation
Endothelial Progenitor Cells
Mitochondrial Membrane Potential

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology

Cite this

Coenzyme Q10 Attenuates High Glucose-Induced Endothelial Progenitor Cell Dysfunction through AMP-Activated Protein Kinase Pathways. / Tsai, Hsiao Ya; Lin, Chih Pei; Huang, Po Hsun; Li, Szu Yuan; Chen, Jia Shiong; Lin, Feng Yen; Chen, Jaw Wen; Lin, Shing Jong.

In: Journal of Diabetes Research, Vol. 2016, 6384759, 2016.

Research output: Contribution to journalArticle

Tsai, Hsiao Ya ; Lin, Chih Pei ; Huang, Po Hsun ; Li, Szu Yuan ; Chen, Jia Shiong ; Lin, Feng Yen ; Chen, Jaw Wen ; Lin, Shing Jong. / Coenzyme Q10 Attenuates High Glucose-Induced Endothelial Progenitor Cell Dysfunction through AMP-Activated Protein Kinase Pathways. In: Journal of Diabetes Research. 2016 ; Vol. 2016.
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