Coculture of endothelial and smooth muscle cells on a collagen membrane in the development of a small-diameter vascular graft

Hsi Chin Wu, Tzu Wei Wang, Pei Leun Kang, Yang Hwei Tsuang, Jui Sheng Sun, Feng Huei Lin

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

In this study, we have evaluated the feasibility of developing a biodegradable collagenous small diameter vascular graft of 2 mm diameter and 1 cm length. In brief, bi-layer type I collagen membrane was fabricated under vacuum suction and lyophilization methods. The smooth muscle cells were inoculated into the lower side of the porous membrane, while endothelial cells were seeded onto upper smooth side of the membrane. After cultured for 7 days, the vascular substitute was either harvested for in vitro examination or in vivo implanted in the subcutaneous layer for biocompatibility test. The tubular vascular prosthesis was then used as a temporary absorbable guide that served as an in vivo vascular graft to promote the complete regeneration of rat inferior vena cava. After implantation for 12 weeks, a thin continuous layer of endothelial cells and smooth muscle cells were lined with the vascular lumen and tunic media, respectively. Histology results showed that there were no signs of significant thrombogeneity and intima hyperplasia. This tissue engineered vascular substitute not only had enough tensile strength and good biocompatibility, but also advanced vascular regeneration. In the future, we suggest that this biodegradable vascular substitute will provide with the possibility in application on small diameter prosthetic grafts in artificial blood vessels.

Original languageEnglish
Pages (from-to)1385-1392
Number of pages8
JournalBiomaterials
Volume28
Issue number7
DOIs
Publication statusPublished - Mar 2007
Externally publishedYes

Fingerprint

Coculture Techniques
Collagen
Grafts
Smooth Muscle Myocytes
Blood Vessels
Muscle
Endothelial cells
Cells
Membranes
Transplants
Biocompatibility
Blood vessel prostheses
Histology
Collagen Type I
Prosthetics
Rats
Tensile strength
Vacuum
Tissue
Regeneration

Keywords

  • Biomimetic material
  • Co-culture
  • Collagen
  • Vascular grafts

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering

Cite this

Coculture of endothelial and smooth muscle cells on a collagen membrane in the development of a small-diameter vascular graft. / Wu, Hsi Chin; Wang, Tzu Wei; Kang, Pei Leun; Tsuang, Yang Hwei; Sun, Jui Sheng; Lin, Feng Huei.

In: Biomaterials, Vol. 28, No. 7, 03.2007, p. 1385-1392.

Research output: Contribution to journalArticle

Wu, Hsi Chin ; Wang, Tzu Wei ; Kang, Pei Leun ; Tsuang, Yang Hwei ; Sun, Jui Sheng ; Lin, Feng Huei. / Coculture of endothelial and smooth muscle cells on a collagen membrane in the development of a small-diameter vascular graft. In: Biomaterials. 2007 ; Vol. 28, No. 7. pp. 1385-1392.
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