Cobalt Chloride Induces Macrophage Foam Cell Formation: A Chemical Hypoxia Model for Anti-Atherosclerotic Drug Screening

Research output: Contribution to journalArticlepeer-review

Abstract

Macrophages would engulf circulating oxidized (ox)-low-density lipoprotein and form lipid droplet-laden foam cells. Macrophage foam cells are considered an important therapeutic target of atherosclerosis. The aim of the study was to investigate a hypoxic foam cell model for anti-Atherosclerotic drug screening using the chemical hypoxia-mimicking agent cobalt chloride (CoCl2). The oil red O stating results showed that treatment with CoCl2 could induce lipid accumulation and lead to cell transformation to spindle-shaped and lipid-rich foam cells in RAW 264.7 macrophages. Incubation with 150 μM CoCl2 for 24 h significantly increased the area of intracellular lipid droplets in macrophages, compared with the control group. Our findings indicate that CoCl2-Triggered macrophage foam cells should be a potential in vitro hypoxia model for atherosclerosis drug discovery.

Original languageEnglish
Pages (from-to)38-45
Number of pages8
JournalAssay and Drug Development Technologies
Volume19
Issue number1
DOIs
Publication statusPublished - Jan 1 2021

Keywords

  • cobalt chloride
  • foam cells
  • lipid droplets
  • macrophages
  • oil red O

ASJC Scopus subject areas

  • Molecular Medicine
  • Drug Discovery

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