Cluster of differentiation 14 and toll-like receptor 4 are involved in the anti-inflammatory effects of tyrosol

Chao Yuan Chang, I. Tao Huang, Hung Jen Shih, Ya Ying Chang, Ming Chang Kao, Ping Chen Shih, Chun Jen Huang

Research output: Contribution to journalArticle

3 Citations (Scopus)


We investigated effects of tyrosol on upstream pathway regulating lipopolysaccharide (LPS)-induced inflammatory response, including cluster of differentiation 14 (CD14)-mediated LPS–macrophage binding, toll-like receptor 4 (TLR4)/myeloid differentiation factor 2 (MD2) expression and subsequent myeloid differentiation primary response gene 88 (MyD88) and TIR-domain-containing adapter-inducing interferon-β (TRIF) recruitments. Flow cytometry revealed that, compared with LPS-treated RAW264.7 cells (LPS group), those treated with LPS and tyrosol (1.2 mM) [LPS+T(1.2) group] demonstrated lower LPS–macrophage binding and membrane-bound CD14 (mCD14) and TLR4/MD2 expression (all p < 0.05). Immunoprecipitation/immunoblotting assay revealed lower MyD88 and TRIF concentrations in the LPS+T(1.2) group than in the LPS group (both p < 0.05). Soluble CD14 concentration was higher in the LPS+T(1.2) group than in the LPS group (p = 0.013); protease inhibition counteracted this effect. Thus, tyrosol inhibits LPS–macrophage binding, mCD14 and TLR4/MD2 expression, and MyD88 and TRIF recruitment, all possibly by enhancing protease-mediated mCD14 cleavage.

Original languageEnglish
Pages (from-to)93-104
Number of pages12
JournalJournal of Functional Foods
Publication statusPublished - Feb 1 2019



  • CD14
  • MyD88
  • RAW264.7 cells
  • TLR4
  • TRIF

ASJC Scopus subject areas

  • Food Science
  • Medicine (miscellaneous)
  • Nutrition and Dietetics

Cite this