Cloning of crystallin from orange-spotted grouper and characterization of its activity as potential protective agent

Young Mao Chen, Cham En Kuo, Chun Mao Lin, Pei Shiuan Shiea, Tzong Yueh Chen

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Oxidative stress associated with nodavirus infection is poorly understood, especially pertaining to infection-mediated brain injury. Indirect evidence indicates that infection increases cellular abundance of reactive oxygen species (ROS) with consequent increase in cellular dityrosine production. The detection of dityrosine in nodavirus-infected grouper was demonstrated using immunohistochemical (IHC) staining. Proteomic analyses with eye tissues of healthy grouper revealed more abundant expression of crystallin protein in the eye than in various tissues, which was confirmed by real-time polymerase chain reaction and IHC analyses. Grouper crystallin belongs to a small heat shock protein family with chaperone-like function that prevents heat-induced and oxidative stress-induced protein aggregation. Recombinant crystallin induced nitric oxide (NO) production in RAW 264.7 cells after treatment. The results provide new insight into the pathogenesis of nodavirus and demonstrate an experimental rationale for antioxidant therapy research.

Original languageEnglish
Pages (from-to)60-69
Number of pages10
JournalResults in Immunology
Volume1
Issue number1
DOIs
Publication statusPublished - 2011

Keywords

  • Crystallin
  • Nodavirus
  • Reactive oxygen species (ROS)

ASJC Scopus subject areas

  • Immunology

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