Cloning and sequencing of cDNA encoding human DNA topoisomerase II and localization of the gene to chromosome region 17q21-22

M. Tsai-Pflugfelder, L. F. Liu, A. A. Liu, K. M. Tewey, J. Whang-Peng, T. Knutsen, K. Huebner, C. M. Croce, J. C. Wang

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Abstract

Two overlapping cDNA clones encoding human DNA topoisomerase II were identified by two independent methods. In one, a human cDNA library in phage λ was screened by hybridization with a mixed oligonucleotide probe encoding a stretch of seven amino acids found in yeast and Drosophila DNA topoisomerase II; in the other, a different human cDNA library in a λgt11 expression vector was screened for the expression of antigenic determinants that are recognized by rabbit antibodies specific to human DNA topoisomerase II. The entire coding sequences of the human DNA topoisomerase II gene were determined from these and several additional clones, identified through the use of the cloned human TOP2 gene sequences as probes. Hybridization between the cloned sequences and mRNA and genomic DNA indicates that the human enzyme is encoded by a single-copy gene. The location of the gene was mapped to chromosome 17q21-22 by in situ hybridization of a cloned fragment to metaphase chromosomes and by hybridization analysis with a panel of mouse-human hybrid cell lines, each retaining a subset of human chromosomes.

Original languageEnglish
Pages (from-to)7177-7181
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume85
Issue number19
Publication statusPublished - 1988
Externally publishedYes

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Type II DNA Topoisomerase
Organism Cloning
Complementary DNA
Chromosomes
Genes
Gene Library
Clone Cells
Chromosomes, Human, Pair 22
Oligonucleotide Probes
Hybrid Cells
Human Chromosomes
Metaphase
Bacteriophages
Drosophila
In Situ Hybridization
Epitopes
Yeasts
Rabbits
Amino Acids
Cell Line

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Cloning and sequencing of cDNA encoding human DNA topoisomerase II and localization of the gene to chromosome region 17q21-22. / Tsai-Pflugfelder, M.; Liu, L. F.; Liu, A. A.; Tewey, K. M.; Whang-Peng, J.; Knutsen, T.; Huebner, K.; Croce, C. M.; Wang, J. C.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 85, No. 19, 1988, p. 7177-7181.

Research output: Contribution to journalArticle

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AU - Tewey, K. M.

AU - Whang-Peng, J.

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