Cloning and sequencing of bullfrog growth hormone complementary DNA

Fu Ming Pan, Wen Chang Chang

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Total mRNA was isolated from the pituitary glands of bullfrog (Rana catesbeiana), purified by affinity chromatography with oligo(dT)-cellulose columns. The cDNA was synthesized and cloned in Escherichia coli using EcoRI linkers and pBR322 as vector. The cDNA library was screened by hybridization with 32P-labeled duck growth hormone (GH) cDNA. A positive clone was selected and sequenced. The full-length bullfrog GH cDNA contains 950 nucleotide pairs with an open reading frame coding for the precursor GH of 215 amino-acid residues. The partial amino-acid sequence from the protein confirms that derived from the cDNA, with Phe as the first residue in the mature bullfrog GH preceded by a 25-residue hydrophobic signal peptide. The bullfrog GH shares sequence homology with those of other vertebrate species in the following order: duck (61% protein sequence homology; 67% cDNA homology), rat (56%; 61%), human (47%; 57%) and salmon (42%; 50%).

Original languageEnglish
Pages (from-to)238-242
Number of pages5
JournalBBA - Gene Structure and Expression
Volume950
Issue number2
DOIs
Publication statusPublished - Jul 13 1988
Externally publishedYes

Fingerprint

Rana catesbeiana
Cloning
Growth Hormone
Organism Cloning
Complementary DNA
Ducks
Affinity chromatography
Amino Acids
Amino Acid Sequence Homology
Salmon
Pituitary Gland
Sequence Homology
Protein Sorting Signals
Gene Library
Affinity Chromatography
Escherichia coli
Open Reading Frames
Vertebrates
Rats
Amino Acid Sequence

Keywords

  • (Bullfrog)
  • Amino acid sequence
  • cDNA sequence
  • Cloning
  • Growth hormone
  • Sequence homology

ASJC Scopus subject areas

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Genetics

Cite this

Cloning and sequencing of bullfrog growth hormone complementary DNA. / Pan, Fu Ming; Chang, Wen Chang.

In: BBA - Gene Structure and Expression, Vol. 950, No. 2, 13.07.1988, p. 238-242.

Research output: Contribution to journalArticle

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