Cloning and sequencing of bullfrog growth hormone complementary DNA

Fu Ming Pan, Wen Chang Chang

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Total mRNA was isolated from the pituitary glands of bullfrog (Rana catesbeiana), purified by affinity chromatography with oligo(dT)-cellulose columns. The cDNA was synthesized and cloned in Escherichia coli using EcoRI linkers and pBR322 as vector. The cDNA library was screened by hybridization with 32P-labeled duck growth hormone (GH) cDNA. A positive clone was selected and sequenced. The full-length bullfrog GH cDNA contains 950 nucleotide pairs with an open reading frame coding for the precursor GH of 215 amino-acid residues. The partial amino-acid sequence from the protein confirms that derived from the cDNA, with Phe as the first residue in the mature bullfrog GH preceded by a 25-residue hydrophobic signal peptide. The bullfrog GH shares sequence homology with those of other vertebrate species in the following order: duck (61% protein sequence homology; 67% cDNA homology), rat (56%; 61%), human (47%; 57%) and salmon (42%; 50%).

Original languageEnglish
Pages (from-to)238-242
Number of pages5
JournalBBA - Gene Structure and Expression
Volume950
Issue number2
DOIs
Publication statusPublished - Jul 13 1988
Externally publishedYes

Keywords

  • (Bullfrog)
  • Amino acid sequence
  • cDNA sequence
  • Cloning
  • Growth hormone
  • Sequence homology

ASJC Scopus subject areas

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Genetics

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