Cloning and sequencing of a carp βs-crystallin cDNA

Tschining Chang, Wen Chang Chang

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

The mRNAs were extracted from common carp (Cyprinus carpio) lenses, purified, reverse transcribed, dC tailed and cloned into Escherichia coli with pBR322 as vector. The cloning efficiency was around 1·107 colonies per μg of mRNA. A clone (pC20) was found by hybrid-arrested translation to contain the cDNA related to carp crystallins. However, comparison of the derived amino-acid sequence with bovine γ-II and βs-crystallins indicates that this carp crystallin sequence resembles closely the bovine βs-crystallin and should be better classified as such except that this fish sequence does not contain the N-terminal 'arm' of four amino-acid residues present in bovine βs-crystallin.

Original languageEnglish
Pages (from-to)89-92
Number of pages4
JournalBBA - Gene Structure and Expression
Volume910
Issue number1
DOIs
Publication statusPublished - Oct 9 1987
Externally publishedYes

Keywords

  • (Carp lens)
  • cDNA cloning
  • Nucleotide sequence
  • β-Crystallin

ASJC Scopus subject areas

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Genetics

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