Cloning and functional expression of a rat kidney extracellular calcium/polyvalent cation-sensing receptor

Daniela Riccardi, Ji Park, Wen Sen Lee, Gerardo Gamba, Edward M. Brown, Steven C. Hebert

Research output: Contribution to journalArticle

415 Citations (Scopus)

Abstract

The maintenance of a stable extracellular concentration of ionized calcium depends on the integrated function of a number of specialized cells (e.g., parathyroid and certain kidney epithelial cells). We recently identified another G protein-coupled receptor (BoPCaR1) from bovine parathyroid that responds to changes in extracellular Ca2+ within the millimolar range and provides a key mechanism for regulating the secretion of parathyroid hormone. Using an homology-based strategy, we now report the isolation of a cDNA encoding an extracellular Ca2+/polyvalent cation-sensing receptor (RaKCaR) from rat kidney. The predicted RaKCaR protein shares 92% identity with BoPCaR1 receptor and features a seven membrane-spanning domain, characteristic of the G protein-coupled receptors, which is preceded by a large hydrophilic extracellular NH2 terminus believed to be involved in cation binding. RaKCaR cRNA-injected Xenopus oocytes responded to extracellular Ca2+, Mg2+, Gd3+, and neomycin with characteristic activation of inositol phospholipid-dependent, intracellular Ca2+-induced Cl- currents. In rat kidney, Northern analysis revealed RaKCaR transcripts of 4 and 7 kb, and in situ hybridization showed localization primarily in outer medulla and cortical medullary rays. Our results provide important insights into the molecular structure of an extracellular Ca2+/polyvalent cation-sensing receptor in rat kidney and provide another basis on which to understand the role of extracellular divalent cations in regulating kidney function in mineral metabolism.

Original languageEnglish
Pages (from-to)131-135
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume92
Issue number1
DOIs
Publication statusPublished - Jan 3 1995
Externally publishedYes

Fingerprint

Organism Cloning
Kidney
Cations
G-Protein-Coupled Receptors
Complementary RNA
Neomycin
Divalent Cations
Xenopus
Phosphatidylinositols
Molecular Structure
Parathyroid Hormone
Oocytes
In Situ Hybridization
Minerals
Complementary DNA
Cell Count
Epithelial Cells
Maintenance
rat extracellular calcium cation-sensing receptor
Calcium

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Cloning and functional expression of a rat kidney extracellular calcium/polyvalent cation-sensing receptor. / Riccardi, Daniela; Park, Ji; Lee, Wen Sen; Gamba, Gerardo; Brown, Edward M.; Hebert, Steven C.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, No. 1, 03.01.1995, p. 131-135.

Research output: Contribution to journalArticle

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