Cloning and characterization of the vasopressin-regulated urea transporter

Guofeng You, Craig P. Smith, Yoshikatsu Kanal, Wen Sen Lee, Matthias Stelzner, Matthias A. Hediger

Research output: Contribution to journalArticle

271 Citations (Scopus)

Abstract

UREA is the principal end product of nitrogen metabolism in mammals'. Movement of urea across cell membranes was originally thought to occur by lipid-phase permeation, but recent studies have revealed the existence of specialized transporters with a low affinity for urea (km, > 200 mM)2. Here we report the isolation of a complementary DNA from rabbit renal medulla that encodes a 397-amino-acid membrane glycoprotein, UT2, with the functional characteristics of the vasopressin-sensitive urea transporter previously described in in vitro-perfused inner medullary collecting ducts3,4. UT2 is not homologous to any known protein and displays a unique pattern of hydrophobicity. Because of the central role of this transporter in fluid balance1,3-7 and nitrogen metabolism8, the study of this protein will provide important insights into the urinary concentrating mechanism and nitrogen balance.

Original languageEnglish
Pages (from-to)844-847
Number of pages4
JournalNature
Volume365
Issue number6449
Publication statusPublished - Oct 28 1993
Externally publishedYes

    Fingerprint

ASJC Scopus subject areas

  • General

Cite this

You, G., Smith, C. P., Kanal, Y., Lee, W. S., Stelzner, M., & Hediger, M. A. (1993). Cloning and characterization of the vasopressin-regulated urea transporter. Nature, 365(6449), 844-847.