Cloning and characterization of the vasopressin-regulated urea transporter

Guofeng You, Craig P. Smith, Yoshikatsu Kanal, Wen Sen Lee, Matthias Stelzner, Matthias A. Hediger

Research output: Contribution to journalArticlepeer-review

281 Citations (Scopus)

Abstract

UREA is the principal end product of nitrogen metabolism in mammals'. Movement of urea across cell membranes was originally thought to occur by lipid-phase permeation, but recent studies have revealed the existence of specialized transporters with a low affinity for urea (km, > 200 mM)2. Here we report the isolation of a complementary DNA from rabbit renal medulla that encodes a 397-amino-acid membrane glycoprotein, UT2, with the functional characteristics of the vasopressin-sensitive urea transporter previously described in in vitro-perfused inner medullary collecting ducts3,4. UT2 is not homologous to any known protein and displays a unique pattern of hydrophobicity. Because of the central role of this transporter in fluid balance1,3-7 and nitrogen metabolism8, the study of this protein will provide important insights into the urinary concentrating mechanism and nitrogen balance.

Original languageEnglish
Pages (from-to)844-847
Number of pages4
JournalNature
Volume365
Issue number6449
Publication statusPublished - Oct 28 1993
Externally publishedYes

ASJC Scopus subject areas

  • General

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