Cloning and characterization of a cDNA encoding an antimicrobial protein from mung bean seeds

Kuan Chung Chen, Ching Yu Lin, Mei Chu Chung, Cheng Chun Kuan, Hsien Yi Sung, Samson C S Tsou, C. George Kuo, Ching San Chen

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15 Citations (Scopus)

Abstract

A cDNA clone, VrCRP (GenBank accession no. AF326687), encoding an antimicrobial protein was isolated from the differentially expressed cDNAs between a pair of near isogenic lines of mid-matured mung bean seeds. Northern blot analysis showed that VrCRP gene is preferentially expressed in bruchid-resistant developing mung bean seeds. The mature VrCRP is a basic cysteine-rich protein with a predicted molecular mass of 5.9 kDa and a calculated isoelectric point of 8.23. Eight cysteine amino acids present in the primary structure of VrCRP compose four disulfide bridges. Biological analysis showed that VrCRP is toxic to E. coli and completely arrested growth of insect cell (Spodoptera frugiperda, Sf21) at a low concentration of 3.42 μM. VrCRP also inhibited the growth of Rhizoctonia solani. Immunolocalization and in situ hybridization indicated that VrCRP was predominantly present in the parenchyma cells of the seed coat. Based on the biological activities and the protein localization, it is suggested that VrCRP may act as an evolutionary barrier in mung bean seeds that is important in coping with invasions of pathogens and herbivores.

Original languageEnglish
Pages (from-to)251-259
Number of pages9
JournalBotanical Bulletin of Academia Sinica
Volume43
Issue number4
Publication statusPublished - Oct 2002
Externally publishedYes

Keywords

  • Antimicrobial protein
  • Immunolocalization
  • In situ hybridization
  • Mung bean

ASJC Scopus subject areas

  • Plant Science

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  • Cite this

    Chen, K. C., Lin, C. Y., Chung, M. C., Kuan, C. C., Sung, H. Y., Tsou, S. C. S., Kuo, C. G., & Chen, C. S. (2002). Cloning and characterization of a cDNA encoding an antimicrobial protein from mung bean seeds. Botanical Bulletin of Academia Sinica, 43(4), 251-259.