Clonal spread of CC17 vancomycin-resistant Enterococcus faecium with multilocus sequence type 78 (ST78) and a novel ST444 in Taiwan

Y. C. Hsieh, W. S. Lee, Tsong Yih Ou, P. R. Hsueh

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

From May 2007 to January 2008, 30 isolates of vancomycin-resistant enterococci (VRE), including 29 Enterococcus faecium (96.7%) and 1 E. faecalis (3.3%) were obtained from various clinical specimens of 30 patients treated at a university hospital in Taiwan. Among these patients, 27 had VRE infections, including urinary tract infection (n=16), bacteremia (n=5), wound infection (n=5), and central nervous system infection (n=1). Three patients had VRE colonization. All of these isolates belonged to the vanA genotype with vancomycin minimum inhibitory concentrations of 64≥128 μg/ml. The isolate of E. faecalis had VanB phenotype-vanA genotype. All these isolates were susceptible to linezolid and were inhibited by tigecycline at 0.25 μg/ml. Multilocus sequence typing (MLST) analysis of the E. faecium isolates showed that 82.8% were ST78, which belongs to lineage C1. Transposon typing classified the 30 isolates of VRE into three types and most of the Tn1546-like elements contained an IS1251-like insertion sequence. Mating experiments showed that the vanA gene clusters were transferable at a frequency of about 10-6 to 10-7. Our findings indicate that nosocomial spread of VRE resulted from dissemination of lineage C1 E. faecium clones, including a novel E. faecium MLST type (ST444), and the horizontal transfer of Tn1546 elements among enterococci.

Original languageEnglish
Pages (from-to)25-30
Number of pages6
JournalEuropean Journal of Clinical Microbiology and Infectious Diseases
Volume29
Issue number1
DOIs
Publication statusPublished - Jan 2010

Fingerprint

Enterococcus faecium
Taiwan
Multilocus Sequence Typing
Linezolid
Genotype
Central Nervous System Infections
Insertional Mutagenesis
Enterococcus
Microbial Sensitivity Tests
Wound Infection
Vancomycin
Multigene Family
Bacteremia
Urinary Tract Infections
Clone Cells
Vancomycin-Resistant Enterococci
Phenotype
Infection

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

Cite this

@article{94b6d103dac047c18d8da4aac33d99b5,
title = "Clonal spread of CC17 vancomycin-resistant Enterococcus faecium with multilocus sequence type 78 (ST78) and a novel ST444 in Taiwan",
abstract = "From May 2007 to January 2008, 30 isolates of vancomycin-resistant enterococci (VRE), including 29 Enterococcus faecium (96.7{\%}) and 1 E. faecalis (3.3{\%}) were obtained from various clinical specimens of 30 patients treated at a university hospital in Taiwan. Among these patients, 27 had VRE infections, including urinary tract infection (n=16), bacteremia (n=5), wound infection (n=5), and central nervous system infection (n=1). Three patients had VRE colonization. All of these isolates belonged to the vanA genotype with vancomycin minimum inhibitory concentrations of 64≥128 μg/ml. The isolate of E. faecalis had VanB phenotype-vanA genotype. All these isolates were susceptible to linezolid and were inhibited by tigecycline at 0.25 μg/ml. Multilocus sequence typing (MLST) analysis of the E. faecium isolates showed that 82.8{\%} were ST78, which belongs to lineage C1. Transposon typing classified the 30 isolates of VRE into three types and most of the Tn1546-like elements contained an IS1251-like insertion sequence. Mating experiments showed that the vanA gene clusters were transferable at a frequency of about 10-6 to 10-7. Our findings indicate that nosocomial spread of VRE resulted from dissemination of lineage C1 E. faecium clones, including a novel E. faecium MLST type (ST444), and the horizontal transfer of Tn1546 elements among enterococci.",
author = "Hsieh, {Y. C.} and Lee, {W. S.} and Ou, {Tsong Yih} and Hsueh, {P. R.}",
year = "2010",
month = "1",
doi = "10.1007/s10096-009-0810-9",
language = "English",
volume = "29",
pages = "25--30",
journal = "European Journal of Clinical Microbiology and Infectious Diseases",
issn = "0934-9723",
publisher = "Springer Verlag",
number = "1",

}

TY - JOUR

T1 - Clonal spread of CC17 vancomycin-resistant Enterococcus faecium with multilocus sequence type 78 (ST78) and a novel ST444 in Taiwan

AU - Hsieh, Y. C.

AU - Lee, W. S.

AU - Ou, Tsong Yih

AU - Hsueh, P. R.

PY - 2010/1

Y1 - 2010/1

N2 - From May 2007 to January 2008, 30 isolates of vancomycin-resistant enterococci (VRE), including 29 Enterococcus faecium (96.7%) and 1 E. faecalis (3.3%) were obtained from various clinical specimens of 30 patients treated at a university hospital in Taiwan. Among these patients, 27 had VRE infections, including urinary tract infection (n=16), bacteremia (n=5), wound infection (n=5), and central nervous system infection (n=1). Three patients had VRE colonization. All of these isolates belonged to the vanA genotype with vancomycin minimum inhibitory concentrations of 64≥128 μg/ml. The isolate of E. faecalis had VanB phenotype-vanA genotype. All these isolates were susceptible to linezolid and were inhibited by tigecycline at 0.25 μg/ml. Multilocus sequence typing (MLST) analysis of the E. faecium isolates showed that 82.8% were ST78, which belongs to lineage C1. Transposon typing classified the 30 isolates of VRE into three types and most of the Tn1546-like elements contained an IS1251-like insertion sequence. Mating experiments showed that the vanA gene clusters were transferable at a frequency of about 10-6 to 10-7. Our findings indicate that nosocomial spread of VRE resulted from dissemination of lineage C1 E. faecium clones, including a novel E. faecium MLST type (ST444), and the horizontal transfer of Tn1546 elements among enterococci.

AB - From May 2007 to January 2008, 30 isolates of vancomycin-resistant enterococci (VRE), including 29 Enterococcus faecium (96.7%) and 1 E. faecalis (3.3%) were obtained from various clinical specimens of 30 patients treated at a university hospital in Taiwan. Among these patients, 27 had VRE infections, including urinary tract infection (n=16), bacteremia (n=5), wound infection (n=5), and central nervous system infection (n=1). Three patients had VRE colonization. All of these isolates belonged to the vanA genotype with vancomycin minimum inhibitory concentrations of 64≥128 μg/ml. The isolate of E. faecalis had VanB phenotype-vanA genotype. All these isolates were susceptible to linezolid and were inhibited by tigecycline at 0.25 μg/ml. Multilocus sequence typing (MLST) analysis of the E. faecium isolates showed that 82.8% were ST78, which belongs to lineage C1. Transposon typing classified the 30 isolates of VRE into three types and most of the Tn1546-like elements contained an IS1251-like insertion sequence. Mating experiments showed that the vanA gene clusters were transferable at a frequency of about 10-6 to 10-7. Our findings indicate that nosocomial spread of VRE resulted from dissemination of lineage C1 E. faecium clones, including a novel E. faecium MLST type (ST444), and the horizontal transfer of Tn1546 elements among enterococci.

UR - http://www.scopus.com/inward/record.url?scp=76849089705&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=76849089705&partnerID=8YFLogxK

U2 - 10.1007/s10096-009-0810-9

DO - 10.1007/s10096-009-0810-9

M3 - Article

VL - 29

SP - 25

EP - 30

JO - European Journal of Clinical Microbiology and Infectious Diseases

JF - European Journal of Clinical Microbiology and Infectious Diseases

SN - 0934-9723

IS - 1

ER -