Clonal diversity of IL-4 and IL-13 expression in human allergen-specific T lymphocytes

D. M. Essayan, W. F. Han, X. M. Li, H. Q. Xiao, J. Kleine-Tebbe, S. K. Huang

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48 Citations (Scopus)

Abstract

The expression of IL-4 and IL-13 was analyzed in a panel of short ragweed allergen (Amb a 1)-specific T-cell clones from an allergic subject and a nonallergic individual. The T cells from the allergic subject showed a predominantly T(n0) phenotype. The T cells from the nonallergic individual produced undetectable levels of IL-4 and high levels of interferon-γ, suggesting a T(n1) cytokine profile. However, all T-cell clones showed significantly higher levels of IL-13 secretion than IL-4 secretion, and no quantitative correlation could be found between the levels of IL-4 and IL-13 in the clones tested. Furthermore, both cytokines showed similar kinetics of expression in antigen-induced steady-state messenger RNA. Finally, both cytokines were induced by stimulation of the cells with either ionomycin alone or with a combination of ionomycin and phorbol myristate acetate. These results demonstrate that there is a significant clonal diversity and quantitative difference in the levels of IL-4 and IL-13 expression in allergen-specific human T cells.

Original languageEnglish
Pages (from-to)1035-1044
Number of pages10
JournalJournal of Allergy and Clinical Immunology
Volume98
Issue number6 I
DOIs
Publication statusPublished - Jan 1 1996
Externally publishedYes

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Keywords

  • Allergen-specific T cell
  • IFN-γ
  • IL-13
  • IL-4

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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