The expression of IL-4 and IL-13 was analyzed in a panel of short ragweed allergen (Amb a 1)-specific T-cell clones from an allergic subject and a nonallergic individual. The T cells from the allergic subject showed a predominantly T(n0) phenotype. The T cells from the nonallergic individual produced undetectable levels of IL-4 and high levels of interferon-γ, suggesting a T(n1) cytokine profile. However, all T-cell clones showed significantly higher levels of IL-13 secretion than IL-4 secretion, and no quantitative correlation could be found between the levels of IL-4 and IL-13 in the clones tested. Furthermore, both cytokines showed similar kinetics of expression in antigen-induced steady-state messenger RNA. Finally, both cytokines were induced by stimulation of the cells with either ionomycin alone or with a combination of ionomycin and phorbol myristate acetate. These results demonstrate that there is a significant clonal diversity and quantitative difference in the levels of IL-4 and IL-13 expression in allergen-specific human T cells.
|Number of pages||10|
|Journal||Journal of Allergy and Clinical Immunology|
|Issue number||6 I|
|Publication status||Published - 1996|
- Allergen-specific T cell
ASJC Scopus subject areas
- Immunology and Allergy