Clonal diversity of IL-4 and IL-13 expression in human allergen-specific T lymphocytes

D. M. Essayan, W. F. Han, X. M. Li, H. Q. Xiao, J. Kleine-Tebbe, S. K. Huang

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

The expression of IL-4 and IL-13 was analyzed in a panel of short ragweed allergen (Amb a 1)-specific T-cell clones from an allergic subject and a nonallergic individual. The T cells from the allergic subject showed a predominantly T(n0) phenotype. The T cells from the nonallergic individual produced undetectable levels of IL-4 and high levels of interferon-γ, suggesting a T(n1) cytokine profile. However, all T-cell clones showed significantly higher levels of IL-13 secretion than IL-4 secretion, and no quantitative correlation could be found between the levels of IL-4 and IL-13 in the clones tested. Furthermore, both cytokines showed similar kinetics of expression in antigen-induced steady-state messenger RNA. Finally, both cytokines were induced by stimulation of the cells with either ionomycin alone or with a combination of ionomycin and phorbol myristate acetate. These results demonstrate that there is a significant clonal diversity and quantitative difference in the levels of IL-4 and IL-13 expression in allergen-specific human T cells.

Original languageEnglish
Pages (from-to)1035-1044
Number of pages10
JournalJournal of Allergy and Clinical Immunology
Volume98
Issue number6 I
DOIs
Publication statusPublished - Jan 1 1996
Externally publishedYes

Fingerprint

Interleukin-13
Interleukin-4
Allergens
T-Lymphocytes
Ionomycin
Clone Cells
Cytokines
Ambrosia
Tetradecanoylphorbol Acetate
Interferons
Phenotype
Antigens
Messenger RNA

Keywords

  • Allergen-specific T cell
  • IFN-γ
  • IL-13
  • IL-4

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Clonal diversity of IL-4 and IL-13 expression in human allergen-specific T lymphocytes. / Essayan, D. M.; Han, W. F.; Li, X. M.; Xiao, H. Q.; Kleine-Tebbe, J.; Huang, S. K.

In: Journal of Allergy and Clinical Immunology, Vol. 98, No. 6 I, 01.01.1996, p. 1035-1044.

Research output: Contribution to journalArticle

Essayan, D. M. ; Han, W. F. ; Li, X. M. ; Xiao, H. Q. ; Kleine-Tebbe, J. ; Huang, S. K. / Clonal diversity of IL-4 and IL-13 expression in human allergen-specific T lymphocytes. In: Journal of Allergy and Clinical Immunology. 1996 ; Vol. 98, No. 6 I. pp. 1035-1044.
@article{970a26eb371d4050aca7ddfe4ab0c99a,
title = "Clonal diversity of IL-4 and IL-13 expression in human allergen-specific T lymphocytes",
abstract = "The expression of IL-4 and IL-13 was analyzed in a panel of short ragweed allergen (Amb a 1)-specific T-cell clones from an allergic subject and a nonallergic individual. The T cells from the allergic subject showed a predominantly T(n0) phenotype. The T cells from the nonallergic individual produced undetectable levels of IL-4 and high levels of interferon-γ, suggesting a T(n1) cytokine profile. However, all T-cell clones showed significantly higher levels of IL-13 secretion than IL-4 secretion, and no quantitative correlation could be found between the levels of IL-4 and IL-13 in the clones tested. Furthermore, both cytokines showed similar kinetics of expression in antigen-induced steady-state messenger RNA. Finally, both cytokines were induced by stimulation of the cells with either ionomycin alone or with a combination of ionomycin and phorbol myristate acetate. These results demonstrate that there is a significant clonal diversity and quantitative difference in the levels of IL-4 and IL-13 expression in allergen-specific human T cells.",
keywords = "Allergen-specific T cell, IFN-γ, IL-13, IL-4",
author = "Essayan, {D. M.} and Han, {W. F.} and Li, {X. M.} and Xiao, {H. Q.} and J. Kleine-Tebbe and Huang, {S. K.}",
year = "1996",
month = "1",
day = "1",
doi = "10.1016/S0091-6749(96)80188-6",
language = "English",
volume = "98",
pages = "1035--1044",
journal = "Journal of Allergy and Clinical Immunology",
issn = "0091-6749",
publisher = "Mosby Inc.",
number = "6 I",

}

TY - JOUR

T1 - Clonal diversity of IL-4 and IL-13 expression in human allergen-specific T lymphocytes

AU - Essayan, D. M.

AU - Han, W. F.

AU - Li, X. M.

AU - Xiao, H. Q.

AU - Kleine-Tebbe, J.

AU - Huang, S. K.

PY - 1996/1/1

Y1 - 1996/1/1

N2 - The expression of IL-4 and IL-13 was analyzed in a panel of short ragweed allergen (Amb a 1)-specific T-cell clones from an allergic subject and a nonallergic individual. The T cells from the allergic subject showed a predominantly T(n0) phenotype. The T cells from the nonallergic individual produced undetectable levels of IL-4 and high levels of interferon-γ, suggesting a T(n1) cytokine profile. However, all T-cell clones showed significantly higher levels of IL-13 secretion than IL-4 secretion, and no quantitative correlation could be found between the levels of IL-4 and IL-13 in the clones tested. Furthermore, both cytokines showed similar kinetics of expression in antigen-induced steady-state messenger RNA. Finally, both cytokines were induced by stimulation of the cells with either ionomycin alone or with a combination of ionomycin and phorbol myristate acetate. These results demonstrate that there is a significant clonal diversity and quantitative difference in the levels of IL-4 and IL-13 expression in allergen-specific human T cells.

AB - The expression of IL-4 and IL-13 was analyzed in a panel of short ragweed allergen (Amb a 1)-specific T-cell clones from an allergic subject and a nonallergic individual. The T cells from the allergic subject showed a predominantly T(n0) phenotype. The T cells from the nonallergic individual produced undetectable levels of IL-4 and high levels of interferon-γ, suggesting a T(n1) cytokine profile. However, all T-cell clones showed significantly higher levels of IL-13 secretion than IL-4 secretion, and no quantitative correlation could be found between the levels of IL-4 and IL-13 in the clones tested. Furthermore, both cytokines showed similar kinetics of expression in antigen-induced steady-state messenger RNA. Finally, both cytokines were induced by stimulation of the cells with either ionomycin alone or with a combination of ionomycin and phorbol myristate acetate. These results demonstrate that there is a significant clonal diversity and quantitative difference in the levels of IL-4 and IL-13 expression in allergen-specific human T cells.

KW - Allergen-specific T cell

KW - IFN-γ

KW - IL-13

KW - IL-4

UR - http://www.scopus.com/inward/record.url?scp=0030444720&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030444720&partnerID=8YFLogxK

U2 - 10.1016/S0091-6749(96)80188-6

DO - 10.1016/S0091-6749(96)80188-6

M3 - Article

VL - 98

SP - 1035

EP - 1044

JO - Journal of Allergy and Clinical Immunology

JF - Journal of Allergy and Clinical Immunology

SN - 0091-6749

IS - 6 I

ER -