Clinical spectrum and molecular characteristics of Klebsiella pneumoniae causing community-acquired extrahepatic abscess

Yee Huang Ku, Yin Ching Chuang, Wen Liang Yu

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Background and Purpose: Klebsiella pneumoniae causes a wide spectrum of infections, including abscess and non-abscess formation. This study investigated the clinical spectrum and molecular characteristics of community-acquired Klebsiella infection with primary extrahepatic abscess. Methods: From April 2004 through March 2007, a total of 18 strains of K. pneumoniae, 11 from blood and 7 from focal purulent specimens, were recovered from a medical center in southern Taiwan. The clinical data were collected from medical records. Hypermucoviscosity phenotype was defined as positive string test. The virulence genes, including rmpA (regulator of mucoid phenotype), magA (specific to K1 capsule serotype), K 2A (specific to K2 capsule serotype), and kfu (an iron uptake system) were amplified by polymerase chain reaction using specific primers. Results: Twelve men and 6 women with ages ranging from 37 to 74 years were enrolled. Fifteen patients had underlying diabetes mellitus. The duration of hospitalization ranged from 1 to 96 days. Three patients died by the end of treatment. All of the K. pneumoniae strains carried rmpA and 16 strains showed the hypermucoviscosity phenotype. Of the 18 strains, 7 strains were positive for k 2A and 4 strains carried magA. kfu was identified in 4 magA-positive strains and 2 magA-negative/k 2A-negative strains. Conclusion: Diabetes mellitus was the most frequent underlying disease among our patients. The rmpA and/ or hypermucoviscosity phenotype were the most common virulence factors in K. pneumoniae isolates causing extrahepatic abscesses, among which K2 capsule serotype (k 2A +) was more prevalent than K1 capsule serotype (magA +).

Original languageEnglish
Pages (from-to)311-317
Number of pages7
JournalJournal of Microbiology, Immunology and Infection
Volume41
Issue number4
Publication statusPublished - Aug 2008

Fingerprint

Klebsiella pneumoniae
Abscess
Capsules
Phenotype
Diabetes Mellitus
Klebsiella Infections
Community-Acquired Infections
Virulence Factors
Taiwan
Medical Records
Virulence
Hospitalization
Iron
Polymerase Chain Reaction
Serogroup
Infection
Genes
Therapeutics

Keywords

  • Bacterial capsules
  • Bacterial proteins
  • Comorbidity
  • Klebsiella pneumoniae
  • Liver abscess
  • Virulence factors

ASJC Scopus subject areas

  • Microbiology (medical)
  • Immunology and Allergy
  • Immunology and Microbiology(all)
  • Infectious Diseases

Cite this

Clinical spectrum and molecular characteristics of Klebsiella pneumoniae causing community-acquired extrahepatic abscess. / Ku, Yee Huang; Chuang, Yin Ching; Yu, Wen Liang.

In: Journal of Microbiology, Immunology and Infection, Vol. 41, No. 4, 08.2008, p. 311-317.

Research output: Contribution to journalArticle

@article{6970942605054e80a56d572e4601eedb,
title = "Clinical spectrum and molecular characteristics of Klebsiella pneumoniae causing community-acquired extrahepatic abscess",
abstract = "Background and Purpose: Klebsiella pneumoniae causes a wide spectrum of infections, including abscess and non-abscess formation. This study investigated the clinical spectrum and molecular characteristics of community-acquired Klebsiella infection with primary extrahepatic abscess. Methods: From April 2004 through March 2007, a total of 18 strains of K. pneumoniae, 11 from blood and 7 from focal purulent specimens, were recovered from a medical center in southern Taiwan. The clinical data were collected from medical records. Hypermucoviscosity phenotype was defined as positive string test. The virulence genes, including rmpA (regulator of mucoid phenotype), magA (specific to K1 capsule serotype), K 2A (specific to K2 capsule serotype), and kfu (an iron uptake system) were amplified by polymerase chain reaction using specific primers. Results: Twelve men and 6 women with ages ranging from 37 to 74 years were enrolled. Fifteen patients had underlying diabetes mellitus. The duration of hospitalization ranged from 1 to 96 days. Three patients died by the end of treatment. All of the K. pneumoniae strains carried rmpA and 16 strains showed the hypermucoviscosity phenotype. Of the 18 strains, 7 strains were positive for k 2A and 4 strains carried magA. kfu was identified in 4 magA-positive strains and 2 magA-negative/k 2A-negative strains. Conclusion: Diabetes mellitus was the most frequent underlying disease among our patients. The rmpA and/ or hypermucoviscosity phenotype were the most common virulence factors in K. pneumoniae isolates causing extrahepatic abscesses, among which K2 capsule serotype (k 2A +) was more prevalent than K1 capsule serotype (magA +).",
keywords = "Bacterial capsules, Bacterial proteins, Comorbidity, Klebsiella pneumoniae, Liver abscess, Virulence factors",
author = "Ku, {Yee Huang} and Chuang, {Yin Ching} and Yu, {Wen Liang}",
year = "2008",
month = "8",
language = "English",
volume = "41",
pages = "311--317",
journal = "Journal of Microbiology, Immunology and Infection",
issn = "0253-2662",
publisher = "Elsevier Taiwan LLC",
number = "4",

}

TY - JOUR

T1 - Clinical spectrum and molecular characteristics of Klebsiella pneumoniae causing community-acquired extrahepatic abscess

AU - Ku, Yee Huang

AU - Chuang, Yin Ching

AU - Yu, Wen Liang

PY - 2008/8

Y1 - 2008/8

N2 - Background and Purpose: Klebsiella pneumoniae causes a wide spectrum of infections, including abscess and non-abscess formation. This study investigated the clinical spectrum and molecular characteristics of community-acquired Klebsiella infection with primary extrahepatic abscess. Methods: From April 2004 through March 2007, a total of 18 strains of K. pneumoniae, 11 from blood and 7 from focal purulent specimens, were recovered from a medical center in southern Taiwan. The clinical data were collected from medical records. Hypermucoviscosity phenotype was defined as positive string test. The virulence genes, including rmpA (regulator of mucoid phenotype), magA (specific to K1 capsule serotype), K 2A (specific to K2 capsule serotype), and kfu (an iron uptake system) were amplified by polymerase chain reaction using specific primers. Results: Twelve men and 6 women with ages ranging from 37 to 74 years were enrolled. Fifteen patients had underlying diabetes mellitus. The duration of hospitalization ranged from 1 to 96 days. Three patients died by the end of treatment. All of the K. pneumoniae strains carried rmpA and 16 strains showed the hypermucoviscosity phenotype. Of the 18 strains, 7 strains were positive for k 2A and 4 strains carried magA. kfu was identified in 4 magA-positive strains and 2 magA-negative/k 2A-negative strains. Conclusion: Diabetes mellitus was the most frequent underlying disease among our patients. The rmpA and/ or hypermucoviscosity phenotype were the most common virulence factors in K. pneumoniae isolates causing extrahepatic abscesses, among which K2 capsule serotype (k 2A +) was more prevalent than K1 capsule serotype (magA +).

AB - Background and Purpose: Klebsiella pneumoniae causes a wide spectrum of infections, including abscess and non-abscess formation. This study investigated the clinical spectrum and molecular characteristics of community-acquired Klebsiella infection with primary extrahepatic abscess. Methods: From April 2004 through March 2007, a total of 18 strains of K. pneumoniae, 11 from blood and 7 from focal purulent specimens, were recovered from a medical center in southern Taiwan. The clinical data were collected from medical records. Hypermucoviscosity phenotype was defined as positive string test. The virulence genes, including rmpA (regulator of mucoid phenotype), magA (specific to K1 capsule serotype), K 2A (specific to K2 capsule serotype), and kfu (an iron uptake system) were amplified by polymerase chain reaction using specific primers. Results: Twelve men and 6 women with ages ranging from 37 to 74 years were enrolled. Fifteen patients had underlying diabetes mellitus. The duration of hospitalization ranged from 1 to 96 days. Three patients died by the end of treatment. All of the K. pneumoniae strains carried rmpA and 16 strains showed the hypermucoviscosity phenotype. Of the 18 strains, 7 strains were positive for k 2A and 4 strains carried magA. kfu was identified in 4 magA-positive strains and 2 magA-negative/k 2A-negative strains. Conclusion: Diabetes mellitus was the most frequent underlying disease among our patients. The rmpA and/ or hypermucoviscosity phenotype were the most common virulence factors in K. pneumoniae isolates causing extrahepatic abscesses, among which K2 capsule serotype (k 2A +) was more prevalent than K1 capsule serotype (magA +).

KW - Bacterial capsules

KW - Bacterial proteins

KW - Comorbidity

KW - Klebsiella pneumoniae

KW - Liver abscess

KW - Virulence factors

UR - http://www.scopus.com/inward/record.url?scp=57649128318&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=57649128318&partnerID=8YFLogxK

M3 - Article

VL - 41

SP - 311

EP - 317

JO - Journal of Microbiology, Immunology and Infection

JF - Journal of Microbiology, Immunology and Infection

SN - 0253-2662

IS - 4

ER -