Clinical significance of signal pattern of high-risk human papillomavirus using a novel fluorescence in situ hybridization assay in cervical cytology

C. M. Ho, B. H. Lee, S. F. Chang, T. Y. Chien, S. H. Huang, C. C. Yan, W. F. Cheng

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The present study aimed to evaluate a novel fluorescence in situ hybridization (FISH) assay for detecting the high-risk human papillomavirus (HR-HPV) DNA and signal pattern in cervical cytology specimens and for identifying cervical intraepithelial neoplasia (CIN) lesions. One hundred and ninety-six liquid-based cytology specimens with CIN were recruited. The signal pattern (punctate, mixed punctate and diffuse, and diffuse) detected by FISH was compared with E6 mRNA and correlated with histological classification. FISH and E6-type specific polymerase chain reaction (PCR) had fair to good agreement for detecting HPV DNA across all grades of CIN (kappa coefficient, 0.37-0.73). Among 44 samples of negative FISH and positive E6 type-specific PCR in HPV 16, 18, 31, 33, 52 and 58, 82% (36/44) of E6 mRNA were not detected, in contrast to 41% (48/118) of positive FISH and positive E6 type-specific PCR (p

Original languageEnglish
Pages (from-to)386-394
Number of pages9
JournalClinical Microbiology and Infection
Volume17
Issue number3
DOIs
Publication statusPublished - Mar 2011

Keywords

  • Cervical intraepithelial neoplasm
  • Fluorescence in situ hybridization
  • High-risk human papillomavirus
  • Signal pattern

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

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